The results of domestic tomato varieties testing with application of growth biostimulant on plant productivity in open ground conditions of the Tersko-Sulak subprovince of Dagestan are presented. The purpose of the research was to study new domestic tomato varieties of the Federal Research Center for Vegetable Growing (FRC VG) selection and to assess the economic efficiency of the application of biopreparation Relict R (liquid). New domestic tomato varieties, bred by scientists-breeders of the Federal Research Center for Vegetable Growing for risky farming zones, in the conditions of southern Russia have shown quite high results on the main economically valuable indicators. Relict R, a preparation based on salts of humic and fulvic acids with growth-stimulating, adaptogenic and protective properties for a wide range of crops, was applied at the plantings. The preparation was developed at the Siberian Branch of the Russian Academy of Sciences. The manufacturing company was OOO RPE “Genesis” (Russia). Tomatoes were grown according to generally accepted technology under open ground conditions. All varieties were early maturing with a growing season duration of 93–110 days. Voskhod VNIISSOK, Blagodatny and Sodruzhestvo varieties showed the best yields (85.3; 83.6 and 78.2 t/ha). All varieties were also distinguished by high early yields (24.2–42.5 t/ha). Dry matter content ranged from 4.1 to 6.9%. The best varieties were Viking, Sodruzhestvo, and Blagodatny, with indexes of 15.2; 13.1; and 8.2, respectively. The ascorbic acid content ranged from 24.85 to 45.20mg%. The Viking variety was distinguished by high content of vitamin C (45.20mg%), as well as vitamins B1, B2, B6, B5 (0.074; 0.058; 0.083; 0.258 mg%). The Magnat variety stood out for the highest content of vitamin B3 (0.511 mg%). Application of the biopreparation significantly increased the yield compared to the control (by 4.1 t/ha), it was higher than the planned yield (80 t/ha). Tomato yield when using Relict R was 85.3 t/ha. The use of biopreparation provided a high positive economic effect – net income and profitability level were higher when using Relict R and amounted to 3928.5 thousand rubles/ha and 12%, respectively. According to the results of the research it is recommended to use humic preparation Relict R (liquid) on tomatoes to increase yield and quality indicators in the conditions of the south of the country.