Biological and Immunogenicity Property of IgY Anti S. mutans ComD

Bachtiar, Endang W.; Soejoedono, Retno Damajanti; Wibawan, I Wayan Teguh; Afdhal, Anggraeni

doi:10.2174/1874210601610010308

Cited by 12 publications

(14 citation statements)

References 24 publications

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“…In addition, specific IgY action against the ComD protein, a quorumsensing signals receptor of S. mutans, was also explored. It inhibited, in vitro, the development of biofilm of S. mutans from the oral cavity of people with and without caries, and changes in the protein expression pattern were found in the bacteria treated with anti-ComD IgY [73].…”

Section: Antibacterial Activitymentioning

confidence: 99%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

133

135

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A B S T R A C TEgg yolk constitutes a relevant alternative source of antibodies. It presents some advantages over mammalian serum immunoglobulins regarding productivity, animal welfare and specificity. The main immunoglobulin present in avian blood (IgY) is transmitted to their offspring and accumulates in egg yolks, which enables the non-invasive harvesting of high amounts of antibodies. Moreover, due to structural differences and phylogenetic distance, IgY is more suitable for diagnostic purposes than mammalian antibodies, since it does not react with certain components of the human immune system and displays greater avidity for mammalian conserved proteins. IgY has been extensively used in health researches, as both therapeutic and diagnostic tool. This article aims to review its applications in both human and veterinary health.

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Section: Antibacterial Activitymentioning

confidence: 99%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

133

135

View full text Add to dashboard Cite

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“…Biofilms comprise communities of various bacterial species that communicate with each other. Biofilm disruption could potentially be achieved by the enhancement of IgY as a bacterial colonization inhibitor utilizing a recombinant DNA technology approach to engineer a vaccine coding for the target protein region of IgY [8]. DNA recombinant could be created by producing specific IgYs for peptide signals as well as intra-and inter-species quorum signal receptors, which are encoded by ComD in S. mutans.…”

Section: Discussionmentioning

confidence: 99%

“…A vaccine containing the ComD coding region of S. mutans has been successfully created by Endang Winiati Bachtiar (patent announcement number 0492779.7). Immunization of chickens with this vaccine produces IgY that disrupts the interaction between dental plaque-forming bacteria in S. mutans colonization [8].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Mouthwash Combination of Immunoglobulin-Y Anti-Comd Streptococcus Mutans and Chitosan on the Formation of Streptococcus Mutans Biofilm

Bachtiar¹,

Apritantia

Sarwono³

et al. 2019

Int J App Pharm

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Objective: The objective of this study was to investigate the effectiveness of immunoglobulin Y (IgY) antibodies as a dental caries vaccine by utilizingIgY specific to the quorum-sensing signaling receptor ComD of Streptococcus mutans combined with chitosan in the form of mouthwash.Methods: The effects of a mouthwash containing IgY anti-ComD S. mutans with and without chitosan on biofilm-forming isolates of S. mutans wereinvestigated. Subjects were assigned to rinsing twice daily for 6 days with 15–20 ml mouthwash solution for 30 s. Biofilm formation of S mutansisolated from the patiens was measured using a crystal violet method to determine the optical density at 490 nm.Results: The results indicated that mouthwash containing IgY anti-ComD S. mutans and chitosan significantly enhanced the biofilm formation ofS. mutans. In mouthwash containing IgY anti-ComD S. mutans without chitosan, a reduction in biofilm formation was observed; however, this was notstatistically significant.Conclusions: The mouthwash combination of IgY anti-ComD S. mutans and chitosan enhanced the biofilm formation ability of S. mutans isolated fromcaries and caries-free subjects. Further, research is needed to determine the appropriate concentrations of IgY anti-ComD S. mutans and chitosanrequired to effectively inhibit dental caries.

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“…Chickens are a suitable model to produce IgY by immunization with nucleic acid [6], protein [7], lipid, and carbohydrates [8]. In addition, an immune humoral response must be elicited by immunization with recombinant proteins [9] or peptides [10].…”

Section: Igy Productionmentioning

confidence: 99%

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

Júnior¹,

Santos²,

Bassi³

et al. 2017

Toxoplasmosis

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In this chapter, we describe relevant aspects of immunoglobulin Y (IgY) technology for Toxoplasma gondii applications, including comparison of avian IgY antibody with mammalian IgG antibody, egg yolk IgY production and isolation procedures, important applications for IgY antibody, and state of the art and perspectives for IgY-technology in T. gondii studies. T. gondii is a worldwide public health problem. IgY-technology provides an alternative antibody (IgY) to mammalian Immunoglobulin G (IgG) antibody. IgY-technology involves the chicken immunization, yolk IgY isolation, antibody characterization, and purified IgY application to several kinds of methods. Immunized chicken transfers a specific IgY from blood to egg yolk. Phylogenetic distance between chickens and mammals influences the generation of antibody repertoires recognizing an antigen profile. IgY is not bound to rheumatoid factor or mammalian complement protein and thus avoids the false-positive results. Yolk IgY isolation is carried out by simple procedures that are accessible for any laboratory and, also, for IgY isolation at large-scale production. IgY-technology provides antibodies for proteomic studies, diagnostic assays, and immunotherapy. Although IgY-technology is promising, there is a reduced number of investigations with IgY and T. gondii. Future perspectives involve the use of IgY-technology for the screening of new T. gondii antigens for diagnostics, therapy, or vaccine, development of innovative techniques for toxoplasmosis diagnostics and may be an immunotherapy for toxoplasmosis.

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Biological and Immunogenicity Property of IgY Anti S. mutans ComD

Cited by 12 publications

References 24 publications

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

The Effect of Mouthwash Combination of Immunoglobulin-Y Anti-Comd Streptococcus Mutans and Chitosan on the Formation of Streptococcus Mutans Biofilm

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

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