Biological and molecular characterization of two tomato strains of potato virus Y (PVY)

Abstract

Two PVY tomato strains (LYE 84 and LYE 84.2), arising from the same natural isolate, and a strain originating from a wild Solanaceous host, Solarium nigrum (SON 41.2), were compared for host range and symptomatology. All strains induced mosaic without necrosis on tobacco as PVY ° strains. The two tomato strains behaved similarly on pepper, infecting only susceptible pepper cultivars (pathotype 0), whereas SON 41.2 was able to overcome the two alleles of the recessive resistance gene pvr2 (pathotype 1.2). On the other hand, only LYE 84.2 was virulent on tomato and broke the resistance of the wild genitor Lycopersicon hirsutum PI 247087. Sequence determination of the capsid gene and the 3' non-coding region of LYE 84 and LYE 84.2 showed a total homology at both nucleic acid and amino acid levels. This suggests that LYE 84.2 has probably derived from LYE 84, that both strains have very similar sequences and that the capsid protein does not play a direct role in the resistance-breaking capacity of LYE 84.2.

Recessive resistance genes against potyviruses are localized in colinear genomic regions of the tomato (Lycopersicon spp.) and pepper (Capsicum spp.) genomes

“…Both genes are overcome by virulent PVY and TEV strains. In order to complete the comparison between pot-1 and pvr2, and to shed light on the Solanaceae-Potyvirus interaction, the resistance-breaking mutations in both PVY and TEV are under study (Morel et al 2000).…”

Potato Virus Y Emergence and Evolution from the Andes of South America to Become a Major Destructive Pathogen of Potato and Other Solanaceous Crops Worldwide

“…Human activity has played a major part in the spread of PVY from the South American Andes to the rest of the world, particularly through trade in plant material of unknown disease status. Since emerging from the Andean region, PVY has also become a major pathogen of tobacco and solanaceous vegetable crops [ 9 , 10 , 11 , 12 , 13 ]. This review highlights current knowledge of PVY population structure, epidemiology and economic impacts, mostly drawn from research on virus infections in potato.…”

Grafting alters tomato transcriptome and enhances tolerance to an airborne virus infection

“…Biological classification of PVY strains is based mostly on the host from which they were isolated 26 . Strong strain-host specificity has been observed in potato and pepper 27 whereas tomato seems poorly selective with respect to symptoms induced by different PVY isolates [27][28][29][30][31][32] . Tomato plants infected by PVY O or PVY C strains show crinkling of young leaves often followed by necrotic mottling and necrosis of the veins on the lower leaf surface while fruits remain usually symptomless 33 .…”