Biological and Molecular Characterization of a New Cucurbit-Infecting Tobamovirus

Antignus, Y.; Wang, Y; Pearlsman, Malenia; Lachman, Oded; Lavi, N; Gal‐On, Amit

doi:10.1094/phyto.2001.91.6.565

Cited by 70 publications

(48 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A survey of literature on virus diseases of cucurbits revealed that more than 39 well characterized viruses are distributed in unrelated genera, such as Begomo virus, Crinivirus, Cucumovirus, Polerovirus, Tospovirus, Ipomovirus, Tobamovirus and Potyvirus, which are known to naturally infect cucurbits (Antignus et al, 2001;Salem et al, 2007;Kneirim et al, 2010;Gholamalizadeh et al, 2008;Brown et al, 2002;Owolabi et al, 2008Owolabi et al, , 2011Owolabi et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Biochemical Changes in Cucurbita moschata Duch. Ex. Poir Inoculated with a Nigerian Strain of Moroccan Watermelon Mosaic Virus (MWMV): Lagenaria breviflora Isolate

Mofunanya¹,

Edu²

2015

International J. of Plant Pathology

View full text Add to dashboard Cite

This study was carried out to determine physiological and biochemical changes in C. moschata inoculated with a Nigerian strain of MWMV isolated from Lagenaria breviflora in Calabar, Nigeria. The virus infection resulted in significant (p#0.05) reductions in plant height, leaf area and number of leaves produces with highest percentage difference of 39.3% (42 PID), 60.9% (35 PID) and 25.7% (49 PID), respectively. There was a significant (p#0.05) difference in chlorophyll content between inoculated and healthy plants. The chlorophyll content of inoculated plants at all stages of growth was lower compared with the healthy plants. Chlorophyll b content was higher than Chl a in both inoculated and healthy plants. Chlorophyll a, b and total Chlorophyll were significantly reduced in inoculated plants when compared with the healthy ones. Of the twelve photochemicals screened, alkaloids, glycosides, saponins, tannins, terpenoids, steroids, polyphenols and reducing sugars were present while phlobatanins, anthraquinones and hydroxymethyl anthraquinones were absent. Moroccan watermelon mosaic virus engendered reductions in alkaloids (32.1%), reducing sugars (42.0%), saponins (33.6%), terpenoids (25.0%) and steroids (15.0%) and increase in inoculated samples of glycosides (14.8%) and flavonoids (12.5%). Effect of MWMV on proximate compositions of C. moschata revealed significant p#0.05) reductions in lipid (25.0%) and ash (14.6%) and significant (p$0.05) increases in protein (23.4%) and fibre (10.5%). Effect of the virus on carotene, vitamin A and C were insignificant. Infection of MWMV also led to significant decreases in the contents of K (48%), Fe (28.6%), Zn (25.0%) and Mg (22.0%), while Cu recorded 100% increase in inoculated samples. Marginal increase in Ca and P were recorded in inoculated samples. Infection of MWMV on antioxidants caused significant increase in inoculated samples of hydrocyanic acid and insignificant increases in total and soluble oxalate and phytic acid. Increases recorded in the antioxidant contents in plants inoculated with MWMV resulted in reduced bioavailability of minerals in the vegetable. Reduction in chlorophyll contents in inoculated plants would be a major factor explaining poor growth of host plants with attendant reductions in mineral elements of C. moschata. Changes in physiological and biochemical parameters induced by MWMV altered vital metabolic processes of C. moschata.

show abstract

Section: Introductionmentioning

confidence: 99%

Physiological and Biochemical Changes in Cucurbita moschata Duch. Ex. Poir Inoculated with a Nigerian Strain of Moroccan Watermelon Mosaic Virus (MWMV): Lagenaria breviflora Isolate

Mofunanya¹,

Edu²

2015

International J. of Plant Pathology

View full text Add to dashboard Cite

show abstract

“…Among those detection methods, RT-PCR (Ugaki et al, 1991;Shim et al, 2005;Yoon et al, 2008;Liu et al, 2009), real time RT-PCR (Chen et al, 2008, transmission electron microcopy (Alshahwan and Abdalla, 1992), immune capture-RTPCR (Celix et al, 1996), ELISA (Antignus et al, 1990;Celix et al, 1996;Mitsuhiro et al, 2006) and monoclonal antibodies (Antignus et al, 2001). To establish procedure of identification and detection, mechanical inoculation, DAS-ELISA, RT-PCR, molecular hybridization and nucleotide sequence were applied in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Precise diagnosis has the prime importance in developing a management strategy of this virus. All these viruses have similar host range and thus cannot be used as a clue for the identification of the virus (Antignus et al, 2001;Yoon et al, 2002). CGMMV was originally described in the UK 1935 (Ainsworth, 1935).…”

Section: Introductionmentioning

confidence: 99%

“…Several strains of CGMMV have been reported from Europe, Pakistan, India and Japan (Komuru et al, 1968; Lee, 1996;Antignus et al, 1990;Francki et al, 1986;Ugaki et al, 1991;Shim et al, 2005;Ko et al, 2006). CGMMV is mechanically and seed transmitted with a narrow host range comprising of cucurbits only and is different in host range from Tobacco mosaic virus (TMV) whose main hosts are members of the solanaceae (Antignus et al, 1990(Antignus et al, , 2001. CGMMV is a rod-shaped, microscopic (300 nm long x 18 nm wide) particle.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

Amer¹

2015

IJAB

View full text Add to dashboard Cite

To cite this paper: Amer, M.A., 2015. Biological and molecular characterization of cucumber green mottle mosaic virus affecting bottle gourd and watermelon plants in Saudi Arabia. AbstractSamples were collected from seven of each bottle gourd and watermelon plants grown in Riyadh and Hail regions of Saudi Arabia in May, 2012 showing symptom suspected to be virus infection and analyzed by a double antibody sandwich against bottle gourd and watermelon viruses. Two of watermelon and three of bottle gourd samples were found to be positive with Cucumber green mottle mosaic virus (CGMMV). However, variable results were obtained with other viruses infecting cucurbits. One sample of CGMMV positive samples from each region were homogenized in a mortar separately and used to make mechanical inoculation on 25 plant species which produced characteristic symptoms of tobamo virus infection. Five ELISA-positive samples from naturally infection were further tested and detected by RT-PCR assay using virus specific primers that amplified a 400 bp fragment in the coat protein gene. The nucleotide sequence of the PCR products from the Saudi isolates of CGMMV isolated from bottle gourd and watermelon were determined and demonstrated its similarity to the other isolates of CGMMV recorded in NCBI. The CP gene of CGMMV-Saudi Arabia isolates shared between 87.2% and 99.7% identity in nucleotide level. The synthesized cDNA probe for CGMMV detection was hybridized with nucleic acid extracts from infected samples collected from the above mentioned locations. This is the first report regarding the genetic makeup of CGMMV at the molecular level in Saudi Arabia.

show abstract

“…Crop yield losses of about 15% have been reported (1,2). CGMMV contains a 6.4 kb (+) ssRNA genome (6).…”

mentioning

confidence: 99%

First report of cucumber green mottle mosaic virus association with the leaf green mosaic disease of a vegetable crop, Luffa acutangula L.

Sharma¹,

Verma²,

Mishra³

et al. 2014

View full text Add to dashboard Cite

Biological and Molecular Characterization of a New Cucurbit-Infecting Tobamovirus

Cited by 70 publications

References 26 publications

Physiological and Biochemical Changes in Cucurbita moschata Duch. Ex. Poir Inoculated with a Nigerian Strain of Moroccan Watermelon Mosaic Virus (MWMV): Lagenaria breviflora Isolate

Physiological and Biochemical Changes in Cucurbita moschata Duch. Ex. Poir Inoculated with a Nigerian Strain of Moroccan Watermelon Mosaic Virus (MWMV): Lagenaria breviflora Isolate

Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

First report of cucumber green mottle mosaic virus association with the leaf green mosaic disease of a vegetable crop, Luffa acutangula L.

Contact Info

Product

Resources

About