First report of cucumber green mottle mosaic virus association with the leaf green mosaic disease of a vegetable crop, Luffa acutangula L.

Sharma, Priya; Verma, Rakesh Kumar; Mishra, Rahul; Sahu, Amit Kumar; Dk, Choudhary; Rk, Gaur

doi:10.4149/av_2014_03_299

Cited by 9 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CGMMV was confirmed to be associated with leaf green mosaic disease in L. acutangula [ 20 ]. Studies on agroinoculation of cDNA infectious clones in cucurbits with TRSV revealed that systemic infection of TRSV occurred in melon, oriental melon, and cucumber plants but not in watermelon and pumpkin [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)

Qi,

Mo,

et al. 2023

Plant Methods

View full text Add to dashboard Cite

Background Virus-induced gene silencing (VIGS) is a reverse genetics technology that can efficiently and rapidly identify plant gene functions. Although a variety of VIGS vectors have been successfully used in plants, only a few reports on VIGS technology in Luffa exist. Results In the present study, a new cucumber green mottle mosaic virus (CGMMV)-based VIGS vector, pV190, was applied to establish the CGMMV-VIGS to investigate the feasibility of the silencing system for Luffa. Phytoene desaturase (PDS) gene was initially selected as a VIGS marker gene to construct a recombinant vector. Plants infected with Agrobacterium harboring pV190-PDS successfully induced effective silencing in Luffa, and an effective gene silencing phenotype with obvious photobleaching was observed. To further validate the efficiency, we selected TEN for gene-silencing, which encodes a CYC/TB1-like transcription factor and is involved in tendril development. Luffa plants inoculated with the pV190-TEN exhibited shorter tendril length and nodal positions where tendrils appear are higher compared to those of non-inoculated plants. RT-qPCR showed that the expression levels of PDS and TEN were significantly reduced in the CGMMV-VIGS plants. Moreover, we evaluated the CGMMV-VIGS efficiency in three cucurbits, including cucumber, ridge gourd, and bottle gourd. Conclusion We successfully established a CGMMV-based VIGS system on ridge gourd and used marker genes to identify the feasibility of the silencing system in Luffa leaves and stems.

show abstract

Section: Discussionmentioning

confidence: 99%

“…CGMMV was reported to be associated with the leaf green mosaic disease of Luffa acutangula [ 20 ]. A system of CGMMV-based VIGS was recently developed, and its applicability to several cucurbits was verified [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)

Qi,

Mo,

et al. 2023

Plant Methods

View full text Add to dashboard Cite

show abstract

“…CGMMV often causes leaf mosaic and malformation, fruit mottling and plant stunting in numerous plant species, including those in the families Solanaceae, Cucurbitaceae and Cruciferae, leading to severe damages to the vegetable industry worldwide [16][17][18]. CGMMV is originally identified in cucumber plant in England, and it is now extensively distributed in Asia, Australia and America [19][20][21][22][23][24][25][26]. Similar to other tobamoviruses, CGMMV is mainly transmitted via contacts and seeds in the field [27].…”

Section: Introductionmentioning

confidence: 99%

Physical contact transmission of Cucumber green mottle mosaic virus by Myzus persicae

Wang

et al. 2021

PLoS ONE

View full text Add to dashboard Cite

Cucumber green mottle mosaic virus (CGMMV), a critical plant virus, has caused significant economic losses in cucurbit crops worldwide. It has not been proved that CGMMV can be transmitted by an insect vector. In this study, the physical contact transmission of CGMMV by Myzus persicae in Nicotiana benthamiana plants was confirmed under laboratory conditions. The acquisition rate increased with time, and most aphids acquired CGMMV at 72 h of the acquisition access period (AAP). Besides, the acquired CGMMV was retained in the aphids for about 12 h, which was efficiently transmitted back to the healthy N. benthamiana plants. More importantly, further experiments suggested that the transmission was mediated by physical contact rather than the specific interaction between insect vector and plant virus. The results obtained in our study contribute to the development of new control strategies for CGMMV in the field.

show abstract

“…Cucumber green mottle mosaic virus (CGMMV) belongs to the genus Tobamovirus (family Cucurbitaceae ) and has a single-stranded, positive-sense RNA genome of about 6.4 kb [1,2]. The infected cucurbit crops exhibit green mottle, mosaic, and uneven symptoms around growing points.…”

Section: Introductionmentioning

confidence: 99%

Visual Detection of Cucumber Green Mottle Mosaic Virus Based on Terminal Deoxynucleotidyl Transferase Coupled with DNAzymes Amplification

Wang

Liu

Zhou

2019

Sensors

View full text Add to dashboard Cite

A simple, rapid, and sensitive visual detection method for observing cucumber green mottle mosaic virus was reported based on the template-independent polymerization activity of terminal deoxynucleotidyl transferase (TdT), coupled with the cascade amplification of Mg2+-dependent DNAzyme and hemin/G-quadruplex DNAzyme. Briefly, the hybridized dsDNA of T1/P1 was cut into two parts at its position of 5′-AA↓CG↑TT-3′ by the restricted enzyme AcII. The longer, newborn fragment originating from P1 was tailed at its 3’-end by oligo dG, and an intact enzymatic sequence of Mg2+-dependent DNAzyme was generated. The substrate sequence in the loop segment of the hairpin probe (HP) hybridized with the newborn enzymatic sequence and was cleaved into two parts in the presence of Mg2+. The locked G-quadruplex sequence in the stem segment of the HP was released, which catalyzed the oxidation of ABTS2- in the presence of H2O2, and the resulting solution turned green. A correlation between the absorbance and concentration of T1 was obtained in a range from 0.1 pM to 2 nM, with a detection limit of 0.1 pM. In addition to promoting a lower detection limit and shorter monitoring time, this method also demonstrated an excellent selectivity to single or double nucleotide changes. Therefore, the designed strategy provided a rapid and efficient platform for viral inspection and plant protection.

show abstract

First report of cucumber green mottle mosaic virus association with the leaf green mosaic disease of a vegetable crop, Luffa acutangula L.

Cited by 9 publications

References 5 publications

Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)

Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)

Physical contact transmission of Cucumber green mottle mosaic virus by Myzus persicae

Visual Detection of Cucumber Green Mottle Mosaic Virus Based on Terminal Deoxynucleotidyl Transferase Coupled with DNAzymes Amplification

Contact Info

Product

Resources

About