Biological and physico-chemical properties of new root canal sealers

Abstract

BackgroundThe purpose of the present study was to compare the biological and the physico-chemical properties of bioceramic-based root canal sealers, calcium hydroxide-based, MTA-based and epoxy resin-based root canal sealers.Material and MethodsTwo bioceramic-based sealers, one calcium hydroxide-based sealer, one MTA-based sealer and two epoxy resin-based sealers were tested.ResultsEasySeal and MTA Fillapex showed severe citotoxic activity, AH Plus and SealapexTM moderate cytotoxicity, BioRoot™ RCS and TotalFill BC Sealer were both cytocompatible. Except for TotalFill BC Sealer, all root canal sealers caused inhibition zones when tested with E. faecalis. The highest inhibition zone was observed for EasySeal, followed by AH Plus. BioRoot™ RCS, SealapexTM and MTA Fillapex showed the lowest inhibition zone. All the tested materials showed different degree of antibacterial activity by using direct contact test (DCT). The highest values were observed for BioRoot™ RCS, TotalFill BC Sealer and EasySeal, followed by MTA Fillapex and SealapexTM. Except for BioRoot RCS and TotalFill BC Sealer, all the root canal sealers fulfilled the requirements of the ISO 6876 standard, demonstrating a weight loss less than 3%. Bioroot RCS, TotalFill BC Sealer and SealapexTM exhibited high alkaline pH with an increase both for BioRoot™ RCS and TotalFill BC Sealer after 24 hours.ConclusionsThe new bioceramic-based sealers showed acceptable physico-chemical properties, but BioRoot™ RCS and TotalFill BC Sealer seems to be too soluble, not respecting ISO 6876 requirements. Key words:Antibacterial activity, cytoxicity, pH, root canal sealers, solubility.

In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

“…TFB and BCR showed similar results in terms of cell viability as compared with a control group. In line with this work, previous reports have described similar results, also using the MTT test with TotalFill BC sealer, which has an almost identical composition, except it changes consistency [25,26]. Benetti et al [27] also reported that BCR is a biocompatible material that induces mineralization in vivo.…”

Effect of immersion in distilled water or phosphate‐buffered saline on the solubility, volumetric change and presence of voids within new calcium silicate‐based root canal sealers

“…, Colombo et al . ). Recently, the new calcium silicate‐based Sealer Plus BC (MK Life, Porto Alegre, RS, Brazil) and Bio‐C Sealer (Angelus, Londrina, PR, Brazil) were developed and introduced.…”

“…Premixed, ready-to-use calcium silicatebased endodontic sealers, such as EndoSequence (Brasseler, Savannah, GA, USA) and TotalFill BC Sealer (FKG Dentaire, La Chaux-de-Fonds, Switzerland), have been studied (Zamparini et al 2019). TotalFill BC has adequate physicochemical properties, but appears to be soluble (Poggio et al 2017, Tanomaru-Filho et al 2017, Colombo et al 2018. Recently, the new calcium silicate-based Sealer Plus BC (MK Life, Porto Alegre, RS, Brazil) and Bio-C Sealer (Angelus, Londrina, PR, Brazil) were developed and introduced.…”

Evolution of bioceramic cements in endodontics

“…Potencial de hidrogeniones (pH): Los productos de tipo MTA tienen un pH inicial de 10,2, que se eleva a 12,5 tres horas después de la mezcla, manteniéndose constante (Torabinejad et al, 1995). Aunque el MTA Fillapex de acuerdo a las evaluaciones observadas por Colombo et al (2018) el pH a las 3 horas posteriores a su aplicación e incubación exhibe un pH inicial de 7,68 que fue seguido por un pH alcalino débil pasadas las 24 horas a 8,02.…”

“…Biocompatibilidad: BioRoot TM RCS es altamente biocompatible, no altera la arquitectura celular, cuya integridad es necesaria para las funciones basales celulares (Loison-Robert et al, 2018). De acuerdo a una investigación realizada recientemente no presenta un efecto citotóxico (Colombo et al, 2018; en contacto directo o indirecto y la genotoxicidad resulto negativa. Tampoco las células expresaban IL-1β o metaloproteinasa de matriz 9 (MMP-9), y la metaloproteinasa 2 de matriz (MMP-2) las cuales están asociadas con respuestas inflamatorias (Loison-Robert et al, 2018).…”