Biological and proteomic characterization of the venom from Peruvian Andes rattlesnake Crotalus durissus

Rodrigues, Carolina Rego; Molina, Denis Alexis Molina; Souza, Dayane N. de; Cárdenas, Javier; Costal-Oliveira, Fernanda; Guerra-Duarte, Clara; Chávez-Olortégui, Carlos

doi:10.1016/j.toxicon.2021.12.008

Cited by 5 publications

(4 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the most diverse classes of esterase is the phospholipase A 2 (PLA 2 ) enzymes, which prefer cleaving glycerophospholipids [ 46 , 47 , 242 ]. In a study completed in 2022 by Rodrigues et al, the PLA 2 enzyme family was found to be the most abundant family within the entire C. durissus venom composition [ 243 ]. While PLA 2 concentration was similar within all Crotalus subspecies, there were still differences between the subspecies, with C. d. durissus having the highest concentration compared to C. d. cumanensis , C. d. ruruima , and C. d. terrificus [ 243 ].…”

Section: Venom Constituents and Biomedical Applicationsmentioning

confidence: 99%

“…In a study completed in 2022 by Rodrigues et al, the PLA 2 enzyme family was found to be the most abundant family within the entire C. durissus venom composition [ 243 ]. While PLA 2 concentration was similar within all Crotalus subspecies, there were still differences between the subspecies, with C. d. durissus having the highest concentration compared to C. d. cumanensis , C. d. ruruima , and C. d. terrificus [ 243 ]. This class of proteins is divided into six families and further classified from I to XVI with capital letters [ 244 ].…”

Section: Venom Constituents and Biomedical Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

A Review of Rattlesnake Venoms

Phan,

Deshwal,

McMahon

et al. 2023

Toxins

View full text Add to dashboard Cite

Venom components are invaluable in biomedical research owing to their specificity and potency. Many of these components exist in two genera of rattlesnakes, Crotalus and Sistrurus, with high toxicity and proteolytic activity variation. This review focuses on venom components within rattlesnakes, and offers a comparison and itemized list of factors dictating venom composition, as well as presenting their known characteristics, activities, and significant applications in biosciences. There are 64 families and subfamilies of proteins present in Crotalus and Sistrurus venom. Snake venom serine proteases (SVSP), snake venom metalloproteases (SVMP), and phospholipases A2 (PLA2) are the standard components in Crotalus and Sistrurus venom. Through this review, we highlight gaps in the knowledge of rattlesnake venom; there needs to be more information on the venom composition of three Crotalus species and one Sistrurus subspecies. We discuss the activity and importance of both major and minor components in biomedical research and drug development.

show abstract

Section: Venom Constituents and Biomedical Applicationsmentioning

confidence: 99%

Section: Venom Constituents and Biomedical Applicationsmentioning

confidence: 99%

A Review of Rattlesnake Venoms

Phan,

Deshwal,

McMahon

et al. 2023

Toxins

View full text Add to dashboard Cite

show abstract

“…Snaclecs are mainly expressed in the venoms of vipers and colubrids [ 73 , 74 ]. Regarding the vipers reported in this review, snaclecs were identified in Bothrops [ 75 , 76 ], Daboia [ 77 ], Crotalus [ 78 ], and Trimeresurus [ 71 ]. In addition, procoagulant proteases, one type of snake-venom serine proteases (SVSPs), were reported to be involved in affecting blood coagulation [ 79 , 80 ].…”

Section: Brain Complicationsmentioning

confidence: 99%

Cerebral Complications of Snakebite Envenoming: Case Studies

Lin

Chen

Liu

et al. 2022

Toxins

View full text Add to dashboard Cite

There are an estimated 5.4 million snakebite cases every year. People with snakebite envenoming suffer from severe complications, or even death. Although some review articles cover several topics of snakebite envenoming, a review of the cases regarding cerebral complications, especially rare syndromes, is lacking. Here, we overview 35 cases of snakebite by front-fanged snakes, including Bothrops, Daboia, Cerastes, Deinagkistrodon, Trimeresurus, and Crotalus in the Viperidae family; Bungarus and Naja in the Elapidae family, and Homoroselaps (rare cases) in the Lamprophiidae family. We also review three rare cases of snakebite by rear-fanged snakes, including Oxybelis and Leptodeira in the Colubridae family. In the cases of viper bites, most patients (17/24) were diagnosed with ischemic stroke and intracranial hemorrhage, leading to six deaths. We then discuss the potential underlying molecular mechanisms that cause these complications. In cases of elapid bites, neural, cardiac, and ophthalmic disorders are the main complications. Due to the small amount of venom injection and the inability to deep bite, all the rear-fanged snakebites did not develop any severe complications. To date, antivenom (AV) is the most effective therapy for snakebite envenoming. In the six cases of viper and elapid bites that did not receive AV, three cases (two by viper and one by elapid) resulted in death. This indicates that AV treatment is the key to survival after a venomous snakebite. Lastly, we also discuss several studies of therapeutic agents against snakebite-envenoming-induced complications, which could be potential adjuvants along with AV treatment. This article organizes the diagnosis of hemotoxic and neurotoxic envenoming, which may help ER doctors determine the treatment for unidentified snakebite.

show abstract

“…A chromatography step might also be added before gel separation to improve resolution and enhance protein coverage [14]. For example, HPLC fractions of Crotalus durissus crude venom were separated using SDS-PAGE under reducing conditions; bands were excised, digested, and subjected to mass spectrometry for protein identification [45]. In addition to proteomic analysis, SDS-PAGE is frequently used during the multidimensional chromatography of SVs.…”

Section: Electrophoretic Techniquesmentioning

confidence: 99%

Separation and Analytical Techniques Used in Snake Venomics: A Review Article

et al. 2022

View full text Add to dashboard Cite

The deleterious consequences of snake envenomation are due to the extreme protein complexity of snake venoms. Therefore, the identification of their components is crucial for understanding the clinical manifestations of envenomation pathophysiology and for the development of effective antivenoms. In addition, snake venoms are considered as libraries of bioactive molecules that can be used to develop innovative drugs. Numerous separation and analytical techniques are combined to study snake venom composition including chromatographic techniques such as size exclusion and RP-HPLC and electrophoretic techniques. Herein, we present in detail these existing techniques and their applications in snake venom research. In the first part, we discuss the different possible technical combinations that could be used to isolate and purify SV proteins using what is known as bioassay-guided fractionation. In the second part, we describe four different proteomic strategies that could be applied for venomics studies to evaluate whole venom composition, including the mostly used technique: RP-HPLC. Eventually, we show that to date, there is no standard technique used for the separation of all snake venoms. Thus, different combinations might be developed, taking into consideration the main objective of the study, the available resources, and the properties of the target molecules to be isolated.

show abstract

Biological and proteomic characterization of the venom from Peruvian Andes rattlesnake Crotalus durissus

Cited by 5 publications

References 57 publications

A Review of Rattlesnake Venoms

A Review of Rattlesnake Venoms

Cerebral Complications of Snakebite Envenoming: Case Studies

Separation and Analytical Techniques Used in Snake Venomics: A Review Article

Contact Info

Product

Resources

About