Biological and serological characterization of viruses of summer squash crops in Yugoslavia

Dukić, Marija; Krstić, Branka; Vico, M Ivana; Katis, N. I.; Papavassiliou, Chryssa; Berenji, B Janos

doi:10.2298/jas0202149d

Cited by 7 publications

(2 citation statements)

References 8 publications

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“…황화엽이 발생되 면 생육이 더디고, 과실 비대가 느려지며 네트 형성이 되지 않고, 당도가 낮아지는 등 상품과 생산이 어렵다 (Park et al, 2011). 이러한 멜론의 황화엽 증상은 재배상 환경조건에 의 해 발생되는 생리적인 원인과 바이러스 이병에 의해 나타나 는 것으로 추정되지만 (Park et al, 2011;Takeshita, 2004 Rajamony et al, 1987), Melon necrotic spot virus(MNSV; Choi et al, 2003;Kim et al, 2008;Kim et al, 2012), Squash mosaic virus(SqMV; Avgelis and Katis, 1989), Papaya ringspot virus (PRSV;Bateson et al, 1994;Grafton-Cardwell et al, 1996) Zucchini yellow mosaic virus (ZYMV; Dukic et al, 2002;Papayiannis et al, 2005;Vucurovic et al, 2009), Kyuri green mottle mosaic virus(KGMMV; Choi, 2001;Tan et al, 2000), Cucurbit aphid-borne yellows virus(CABYV; Guilley et al, 1994) 등 39종 이상의 바이러 스에 감염되는 것으로 알려져 있다 (Lecoq et al, 2003). 그 중에서도 CABYV는 프랑스에서 1992년에 처음 발생이 보 고되었고 (Lecoq, 1999), CABYV 감염 멜론의 경우 40% 정 도의 상품 수량이 감소한다고 보고된 바 있지만 (Lecoq, 1999;Lecoq et al, 2012 분석한 결과 CABYV 일본에서 보고된 바이러스와 상동성 이 높은 것으로 확인되었다( Table 2).…”

Section: 서는 몇 년 전부터 잎이 황화되는 증상이 발생하기 시작하 였는데 그 발생 지역과 면적이 점차 확대되면서 멜unclassified

Physiological Characteristics of Melon Plants Showing Leaf Yellowing Symptoms Caused by CABYV Infection

이희주¹,

김미경²,

이상규³

et al. 2015

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Melon leaves showing yellowing symptoms were analyzed using electron microscopy and RT-PCR for major cucurbitinfecting-viruses (CMV, MNSV, CGMMV, SqMV, WMV, KGMMV, PRSV and ZYMV) reported in Korea, but these viruses were not detected. As the result of further analysis by next-generation sequencing (NGS), the virus was identified as Cucurbit aphid-borne yellows virus (CABYV), and then confirmed by RT-PCR using CABYV-specific primers. When photosynthetic capacity was measured based on chlorophyll fluorescence yield (ChlFY), the leaves of the diseased plants showed 4.09 µmol･m ). Cytological observations revealed that there were no morphological differences in the palisade parenchyma and mesophyll spongy cells of the leaves between the diseased and the normal plants. However, the same leaf cells of the affected plants contained more starch granules compared to those of the normal, unaffected plants. We conclude that the LYS of muskmelon is not merely a physiological disorder but a viral disease caused by CABYV and spread by aphids.

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Section: 서는 몇 년 전부터 잎이 황화되는 증상이 발생하기 시작하 였는데 그 발생 지역과 면적이 점차 확대되면서 멜unclassified

Physiological Characteristics of Melon Plants Showing Leaf Yellowing Symptoms Caused by CABYV Infection

이희주¹,

김미경²,

이상규³

et al. 2015

HST

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“…Disease epidemics develop through primary inoculum. The main source of primary inoculum is weed host in which virus persist throughout the season [14]. In some crop, the virus is transmitted through planting materials, seeds and through weeds [15].…”

Section: Introductionmentioning

confidence: 99%

Serological and Molecular Identification Based on Coat Protein (CP) Gene of Cucumber mosaic Virus (CMV) Infecting Cucumber (Cucumis sativus L) in Pothwar Region of Pakistan

Asad¹,

Ahsan²,

Ashfaq³

2019

J Plant Biochem Physiol

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Cucumber mosaic virus (CMV) is one of the most important plant viruses and a major threat to a wide range of hosts. Prevalence of CMV in Pakistan is alarming for vegetable production especially cucurbits. The present study was done to estimate the prevalence, distribution as well as coat protein base identification of this notorious virus. During 2015-16 incidence of CMV was recorded in cucumber field in the Pothwar region of Pakistan (Rawalpindi, Attock, Jhelum, Chakwal, and Islamabad). During survey 150 samples were collected and tested through DAS-ELISA (Double Antibody Sandwiched Enzyme Linked Immunosorbent Assay). Results show that CMV prevails throughout the region. Maximum disease incidence was recorded in Rawalpindi (50%) followed by Chakwal (46%), Attock (43%), Islamabad (40%) and Jhelum (36%). Virus infectivity was assayed by indicator plants (Capsicum annuum, Cucumis sativus cv, Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum, and Datura stramonium) through mechanical inoculation. Upon mechanical inoculation, plants show Chlorotic lesion, Necrotic lesion, Mosaic, Stunting, Spots. Coat protein (CP) gene-specific forward (CMVF-45) and reverse (CMVR-45) primer amplified 500bp fragments through Polymerase Chain Reaction (PCR).

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Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

et al. 2012

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Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed.ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.

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Biological and serological characterization of viruses of summer squash crops in Yugoslavia

Cited by 7 publications

References 8 publications

Physiological Characteristics of Melon Plants Showing Leaf Yellowing Symptoms Caused by CABYV Infection

Physiological Characteristics of Melon Plants Showing Leaf Yellowing Symptoms Caused by CABYV Infection

Serological and Molecular Identification Based on Coat Protein (CP) Gene of Cucumber mosaic Virus (CMV) Infecting Cucumber (Cucumis sativus L) in Pothwar Region of Pakistan

Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

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