Biological Behavior of Dextran-Iron Oxide Magnetic Fluid Injected Intravenously in Rats

Abstract

Dextran-magnetic iron oxide complex (CMDM) consisting of carboxymethyl dextran and a magnetite core has been synthesized. The physical and biological properties were compared with those of the conventional dextran-magnetite complex (ATDM). The CMDM exhibits a super-paramagnetic property, has high stability and low toxicity. The uptake and metabolic behavior of CMDM in live rats after intravenous (iv) administration were studied using pulsed nuclear magnetic resonance, optical microscopy and transmission electron microscopy. The ultrafine particles of CMDM were clearly observed in the lysosomes of Kupffer cells in the liver and of macrophages in the spleen. The CMDM accumulated in the lysosomes was found to disappear within two weeks after the iv-injection. The results obtained in this work suggest that CMDM is appropriate for use as a contrast agent for magnetic resonance imaging (MRI).

Investigation of the interaction between magnetic nanoparticles surface-coated with carboxymethyldextran and blood cells using Raman spectroscopy

“…To understand this improvement in the oxy/deoxy ratio for the blood-doped samples, after dispersing CMDM in blood mice, it is interesting consider the kind of the nanoparticle coat. Magnetite nanoparticle is coated by a molecular chain of carboxymethyldextran that binds to the magnetite core at several points on the core surface by COO À terminal [10]. The presence of the no bond COO À terminal, on the magnetite nanoparticle surface may be to satisfy this demand that oxygen status varies on the blood-doped samples.…”

“…The particle size distribution profile of the CMDM sample was obtained from transmission electron microscopy (TEM) using the standard approach, i.e. the log-normal distribution function [9], with an average nanoparticle diameter of D m ¼ 8:9 AE 0:1 nm and a standard deviation in size of s ¼ 0:21 AE 0:01: Blood clearance curves, obtained by Hasegawa et al [10], show that CMDM are completely removed from the blood stream 24 h after injection, whereas alkali-treated dextran is out of circulation 8 h after injection.…”

In vivo monitoring of rat macrophages labeled with poly(l‐lysine)‐iron oxide nanoparticles

“…8 The majority of superparamagnetic iron oxides recently investigated was coated with polysaccharides, namely dextran and its derivatives, to provide steric stabilization. [24][25][26] However, these particles did not show sufficient cellular uptake to enable cell tracing, probably because of insufficient stimulation of endocytosis by interaction with membrane receptors, due to the inert nature of dextran. 27 Alternative stabilization of magnetic nanoparticles involves electrostatic repulsion of neighboring particles.…”

Evaluation of selective tumor detection by clinical magnetic resonance imaging using antibody-conjugated superparamagnetic iron oxide

“…Therefore, in this study we prepared appropriate controls, and conducted experiments by applying widely-used clinical (1.5-Tesla) MRI apparatus, and confirmed the results with the experimental (7.0-Tesla) MRI apparatus in order to determine accurate tumor-targeting specificity of mAb-conjugated SPIO in the present study. Targeting using Clinical MRI 3 To determine accurate tumor-targeting specificity in vivo by clinical MRI, we utilized an MRI detection system using mAb-conjugated carboxymethyl dextran magnetite (CMDM) [12], a dextran-coated superparamagnetic iron oxide (SPIO). Antibodies with internalizing functions are particularly effective for tumor targeting and therapeutics [13][14].…”