Biological behavior of neurally differentiated periodontal ligament stem cells on different titanium implant surfaces

Li, Xiaojie; Liao, Dapeng; Gong, Ping; Dong, Yan; Sun, Gang

doi:10.1002/jbm.a.34953

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…In addition, Adrb2 expression was further confirmed in isolated and expanded PDLCs derived from the neural crest where the SNS originated. PDL-derived stem cells express neural crest cell–specific markers and can be induced to form neural crest-like cells (Coura et al , 2008; Dupin and Coelho-Aguiar, 2013; Fortino et al , 2014; Xiaojie et al , 2014), which implies a close connection between PDLCs and SNS in Adrb2 expression. Static compressive force in vitro upregulated the expression of Adrb2 in PDLCs as force was increased and force-treated time was prolonged.…”

Section: Discussionmentioning

confidence: 99%

Force-induced Adrb2 in Periodontal Ligament Cells Promotes Tooth Movement

Cao¹,

Kou²,

Yang³

et al. 2014

J Dent Res

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The sympathetic nervous system (SNS) regulates bone resorption through β-2 adrenergic receptor (Adrb2). In orthodontic tooth movement (OTM), mechanical force induces and regulates alveolar bone remodeling. Compressive forceassociated osteoclast differentiation and alveolar bone resorption are the rate-limiting steps of tooth movement. However, whether mechanical force can activate Adrb2 and thus contribute to OTM remains unknown. In this study, orthodontic nickel-titanium springs were applied to the upper first molars of rats and Adrb1/2 -/-mice to confirm the role of SNS and Adrb2 in OTM. The results showed that blockage of SNS activity in the jawbones of rats by means of superior cervical ganglion ectomy reduced OTM distance from 860 to 540 μm after 14 d of force application. In addition, the injection of nonselective Adrb2 agonist isoproterenol activated the downstream signaling of SNS to accelerate OTM from 300 to 540 μm after 7 d of force application. Adrb1/2 -/-mice showed significantly reduced OTM distance (19.5 μm) compared with the wild-type mice (107.6 μm) after 7 d of force application. Histopathologic analysis showed that the number of Adrb2-positive cells increased in the compressive region of periodontal ligament after orthodontic force was applied on rats. Mechanistically, mechanical compressive force upregulated Adrb2 expression in primary-cultured human periodontal ligament cells (PDLCs) through the elevation of intracellular Ca 2+ concentration. Activation of Adrb2 in PDLCs increased the RANKL/OPG ratio and promoted the peripheral blood mononuclear cell differentiation to osteoclasts in the cocultured system. Upregulation of Adrb2 in PDLCs promoted osteoclastogenesis, which accelerated OTM through Adrb2-enhanced bone resorption. In summary, this study suggests that mechanical force-induced Adrb2 activation in PDLCs contributes to SNS-regulated OTM.

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Section: Discussionmentioning

confidence: 99%

Force-induced Adrb2 in Periodontal Ligament Cells Promotes Tooth Movement

Cao¹,

Kou²,

Yang³

et al. 2014

J Dent Res

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“…They used PDLSCs instead of SCs due to difficulties obtaining pure and ample sources of SCs. The study showed that the differentiated PDLSCs had the highest expression of SC markers and proteins on the SA titanium surface [ 79 ].…”

Section: Peripheral Nerve System Treatmentmentioning

confidence: 99%

Periodontal ligament stem cells as a promising therapeutic target for neural damage

et al. 2022

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Background The damaged neuronal cells of adult mammalian lack the regenerative ability to replace the neuronal connections. Periodontal ligament stem cells (PDLSCs) are the promising source for neuroregenerative applications that can improve the injured microenvironment of the damaged neural system. They provide neuronal progenitors and neurotrophic, anti-apoptotic and anti-inflammatory factors. In this study, we aimed to comprehensively explore the various neuronal differentiation potentials of PDLSCs for application in neural regeneration therapy. Main text PDLSCs have superior potential to differentiate into various neural-like cells through a dedifferentiation stage followed by differentiation process without need for cell division. Diverse combination of nutritional factors can be used to induce the PDLSCs toward neural lineage. PDLSCs when coupled with biomaterials could have significant implications for neural tissue repair. PDLSCs can be a new clinical research target for Alzheimer's disease treatment, multiple sclerosis and cerebral ischemia. Moreover, PDLSCs have beneficial effects on retinal ganglion cell regeneration and photoreceptor survival. PDLSCs can be a great source for the repair of injured peripheral nerve through the expression of several neural growth factors and differentiation into Schwann cells. Conclusion In conclusion, these cells are an appealing source for utilizing in clinical treatment of the neuropathological disorders. Although significant in vitro and in vivo investigations were carried out in order for neural differentiation evaluation of these cells into diverse types of neurons, more preclinical and clinical studies are needed to elucidate their therapeutic potential for neural diseases.

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“…[3][4][5] In our previous study, we found that periodontal ligament stem cells (PDLSCs) isolated from beagle dogs effectively differentiated into SCs with exposure to a combination of dimethyl sulfoxide (DMSO), basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and forskolin. 6,7 Multiple signaling pathways, transcription factors, and neurotrophic factors had been implicated in their differentiation. 3,8 However, the mechanisms underlying the process of PDLSC differentiation to SCs are poorly characterized.…”

Section: Introductionmentioning

confidence: 99%

Notch pathway activation promotes the differentiation of beagle dog periodontal ligament stem cells to Schwann cells

Liao

Sun

et al. 2021

Adv Clin Exp Med

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Background. Periodontal ligament stem cells (PDLSCs) have demonstrated the potential for differentiation into many cell types, though the molecular mechanism of their neural differentiation in particular remains largely unknown.Objectives. The Notch signaling pathway plays a key role in regulating cell differentiation and development.In this article, we explore its potential role in the differentiation of PDLSCs to Schwann cells (SCs). Materials and methods.The PDLSCs were either transfected with viral vectors carrying genetic material for Notch Delta ligands, thereby induced their overexpression, or treated with DAPT (a Notch-pathway-specific inhibitor) to inhibit γ-secretase. The potential effects of Notch signaling on myelination and SCs differentiation were then investigated using western blotting, immunostaining and reverse transcriptase polymerase chain reaction (RT-PCR) to detect the expression of SC-specific marker genes.Results. Specifically inhibiting Notch signaling with DAPT decreased the expression of SC-specific marker genes GFAP, S100 and P75, as well as of SC-myelin-related genes PMP22, MBP, connexin, and P0 in cells undergoing induced differentiation from PDLSCs. Conversely, activating Notch signaling through overexpression of Delta ligands enhanced the expression of SC-specific marker genes as well as myelin-related genes in cells undergoing induced differentiation from PDLSCs. This promotion was reversed by DAPT.Conclusions. The Notch signaling pathway positively regulated the process of PDLSC differentiation into SCs, and the activation of this signaling was important in maintaining the differentiation of PDLSCs to SCs, and then SC myelination. These results may improve the method of obtaining pure SCs from PDLSCs for transplantation application.

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Biological behavior of neurally differentiated periodontal ligament stem cells on different titanium implant surfaces

Cited by 7 publications

References 18 publications

Force-induced Adrb2 in Periodontal Ligament Cells Promotes Tooth Movement

Force-induced Adrb2 in Periodontal Ligament Cells Promotes Tooth Movement

Periodontal ligament stem cells as a promising therapeutic target for neural damage

Notch pathway activation promotes the differentiation of beagle dog periodontal ligament stem cells to Schwann cells

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