Biological Behaviour and DNA Cytophotometry of Urothelial Bladder Carcinoma

Hofstaedter, Ferdinand; Jakse, G.; Lederer, B; Mikuz, Gregor; Delgado, Ricardo Revilla

doi:10.1111/j.1464-410x.1984.tb05389.x

Cited by 28 publications

(12 citation statements)

References 20 publications

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“…The present study confirms these previous results and shows that a low percentage of stemline values is a prognostic parameter also in diploid tumours. The obser vations of Hofstaedter et al [4], Tribukait et al [6] and Gustafson et al [9] which show a favourable prognosis for bladder cancer patients with a low proliferative activity compared with those with a high proliferation rate are thus indirectly confirmed. Collste et al [10], on the other hand, could not detect any prognostic significance of the proliferative activity as determined by flow cytometry.…”

Section: Methodssupporting

confidence: 62%

“…Transitional cell carcinoma of the urinary bladder has been one of the most frequently studied cancer types. Non-diploidy is usually correlated with histological high grade and advanced stage, whereas well-differentiated and/or low stage tumours most frequently are diploid [2][3][4], Due to this correlation it is obvious that patients with diploid bladder tumours have a better prognosis, whereas the outcome is worse for non-diploid tumours. Few stud ies have regarded the final clinical outcome of bladder cancer patients in relation to nuclear DNA content, espe cially if subgroups with comparable clinical stages and/or comparable histological grades are taken into consider ation [2], In addition, there have been very few long-term studies dealing with the prognostic significance of nuclear DNA in human bladder cancer.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Prognostic Relevance of Nuclear Feulgen DNA in Transitional Cell Carcinoma of the Urinary Bladder

Fosså¹,

Kaalhus²

1985

Eur Urol

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Section: Methodssupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

The Prognostic Relevance of Nuclear Feulgen DNA in Transitional Cell Carcinoma of the Urinary Bladder

Fosså¹,

Kaalhus²

1985

Eur Urol

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“…Since there is evidence that the malignancy of cells increases with the degree of aneuploidy (Hofstaedter et al, 1984), the results suggest that during cultivation an enrichment of the most malignant cells occurs. They also show that, in general, colonies have an S phase fraction similar to, or larger than, that of the tumours of origin.…”

Section: Discussionmentioning

confidence: 85%

Selection of tumour cell subpopulations occurs during cultivation of human tumours in soft agar. A DNA flow cytometric study

Pettersen¹,

Fosså²,

Pihl³

1985

Br J Cancer

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Summary To examine whether selection of tumour cell subpopulations occurs during cultivation in soft agar, we compared in 23 human tumours of different histological types the DNA content of cells from colonies formed in soft agar (method of Courtenay and Mills, 1978) The paramount problem encountered by most workers using semi-solid media for cell cultivation is the low yield of colonies. Thus, colony formation is achieved in less than half of the tumours, and, with few exceptions (Tveit et al., 1982), the plating efficiencies (PEs) obtained are in the range 0.001-0.1%. A PE of 0.01% implies that only 1 out of 104 cells is forming a colony. The low PEs raise the question whether the culture conditions select particular subpopulations of tumour cells.Since tumour cell subpopulations may have specific characteristics that do not necessarily reflect those of the stem cells, it is important to establish whether a selection occurs during growth in soft agar. Previously only a few reports have addressed this question and examined the properties of the colonies formed in vitro (Carney et al., 1981; Persky et al., 1982;Salmon, 1980; Thomson & Meyskens, 1982;Trent, 1980;Tveit et al., 1982). In the present Materials and methods TumoursSurgically removed tumours were immediately put in cold (4°C) Hams F12 medium and transported to the laboratory. Normal and necrotic elements were removed. Tumour tissue was cut into pieces of 2-5mm, mixed with 10-20ml complete medium (Hams F12 medium supplemented with 15% foetal calf serum, glutamine and penicillin and streptomycin) in a plastic bag, and mechanically disaggregated in a stomacher (Lab-Blender 80, Seward Laboratory, London) for 30s. A pure single-cell suspension was obtained by filtration of the cells through a 45pim nylon mesh. Infrequently clusters of 2 or 3 cells penetrated this mesh, but they could easily be removed in a 30 gm mesh.The single cell suspension was centrifuged, the cells were resuspended in fresh medium and the number of viable cells scored. A cytospin preparation was made for cytological examinations. Another aliquot was processed for DNA flow cytometric measurements by centrifugation and resuspension in citrate buffer. The cells were frozen at -70°C. A third part of the cell suspension was used for cultivation in agar.

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“…The few studies on urothelial tumors conducted to date, most of which have used flow cytometry, have obtained varying results and report high DNA values in poorly differentiated uro thelial carcinomas of the bladder [8,[10][11][12][13][17][18][19],…”

Section: Discussionmentioning

confidence: 99%

Prognostic Relevance of DNA Ploidy and Proliferative Activity in Urothelial Carcinoma of the Renal Pelvis and Ureter

Borgmann

Al-Abadi²,

Nagel³

1991

Urol Int

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In 55 patients with urothelial carcinoma of the renal pelvis or ureter, the ploidy, the DNA heterogeneity and the counts of cell cycle phases in the tumor were examined by means of single-cell DNA cytophotometry in order to find more prognostic factors than those already known (stage and grade). Follow-up periods ranged from 1 to 6 years. At the time of first diagnosis, 42 (76%) of the patients had tumors of the renal pelvis, 13 (24%) of them had ureteral tumors. 23 (42%) patients were in stage pT 1 N 0, 15 (27%) in stage pT 2 N 0, 12 (22%) in stage pT 3 N 0, and 5 (9%) were in stage pT 3 N +. The histological malignancy grade most frequently seen in the patients examined – i.e. in 51% of cases – was malignancy grade II. 25% of the patients had grade III tumors whereas only 24% had grade I tumors. With malignancy grade I, DNA cytophotometry showed DNA frequency peaks to be in the diploid range while tumors with malignancy grade II showed heterogenous DNA patterns. 71% of the patients with malignancy grade III showed aneuploid DNA values; 29% of them had polyploid DNA values. For malignancy grades II and III, the proliferation rate of the tumor cells was statistically significantly higher than for malignancy grade I. The determination of tumor heterogeneity and tumor cell proliferation by means of DNA cytophotometry gives valuable clues regarding prognosis.

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Biological Behaviour and DNA Cytophotometry of Urothelial Bladder Carcinoma

Cited by 28 publications

References 20 publications

The Prognostic Relevance of Nuclear Feulgen DNA in Transitional Cell Carcinoma of the Urinary Bladder

The Prognostic Relevance of Nuclear Feulgen DNA in Transitional Cell Carcinoma of the Urinary Bladder

Selection of tumour cell subpopulations occurs during cultivation of human tumours in soft agar. A DNA flow cytometric study

Prognostic Relevance of DNA Ploidy and Proliferative Activity in Urothelial Carcinoma of the Renal Pelvis and Ureter

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