Biological changes during the adaptation of an anaerobic digester to a synthetic substrate

Hattingh, W.H.J.; Kotzé, J.P; Thiel, Pieter G.; Toerien, D.F.; Siebert, M.L.

doi:10.1016/0043-1354(67)90003-6

Cited by 35 publications

(9 citation statements)

References 11 publications

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“…As has been reported by several authors (Anderson et al, 1994;Britz et al, 1994;Hattingh et al, 1967;Zhang and Noike, 1994), several factors (operational and nutritional) affect the relative microbial population of anaerobic sludges. It is already known, that the acclimation of an anaerobic sludge to a specific substrate leads to a mixture of bacterial populations that may be quite different from that of the mother culture.…”

Section: Introductionmentioning

confidence: 58%

“…It is already known, that the acclimation of an anaerobic sludge to a specific substrate leads to a mixture of bacterial populations that may be quite different from that of the mother culture. Consequently, this new culture is characterized by a modified ability for the consumption of various substrates (Aguilar et al, 1995;Breure et al, 1986aBreure et al, , 1986bBritz et al, 1994;Chynoweth et al, 1971;Gavala et al, 1999;Hattingh et al, 1967;Sorensen et al, 1991). Nevertheless, there are a surprisingly small number of systematic studies that quantify this differentiation using kinetic expressions and modeling.…”

Section: Introductionmentioning

confidence: 99%

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Influence of anaerobic culture acclimation on the degradation kinetics of various substrates

Gavala

Lyberatos

2001

Biotech & Bioengineering

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The adaptation of an anaerobic culture (anaerobic sludge) to a specific substrate brings significant changes to its microbial population. These changes can be described by the sludge's ability to treat various substrates such as carbohydrates or proteins or "intermediate" products of anaerobic metabolism such as L-lactic, propionic, and acetic acids. The activity of the sludge with respect to a specific substrate is a critical parameter, because the anaerobic degradability of wastewaters depends strongly on it. This work examines and quantifies the differentiation of two anaerobic sludges of the same origin, following an adaptation period of about 18 months to lactose and gelatin, respectively. The acclimation has a significant effect on the maximum specific utilization rates of various compounds and on their apparent consumption kinetics. It is noticeable, however, that even if the anaerobic cultures were not exposed to a specific substrate for a prolonged period of time (more than a year), they still kept the ability of hydrolyzing or degrading it. In addition, the acclimation has an unquestionable effect on the stoichiometry of the production of volatile fatty acids and L-lactate. Finally, from codigestion experiments it is shown that codigestion of lactose and gelatin appears to have no effect on their hydrolysis kinetics in any of the lactose or gelatin acclimated cultures; specifically, the hydrolysis kinetics remained the same as calculated when lactose or gelatin were the only fed substrates. Similarly, the kinetics of L-lactate and D-glucose biodegradation seemed to be unchanged. On the other hand, codigestion has a significant effect on the production of L-lactic, propionic, and acetic acids, which can be attributed to the increased hydrogen production accompanying gelatin biodegradation.

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Section: Introductionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Influence of anaerobic culture acclimation on the degradation kinetics of various substrates

Gavala

Lyberatos

2001

Biotech & Bioengineering

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“…The fluctuations in enzyme activities of the experimental anaerobic digester referred to in the introduction may be compared with those found in the present study. This digester was adapted over a period of 60 days from a feed of raw-sewage sludge to a synthetic substrate (Hattingh et al 1967), and subsequently received a constant daily load of the synthetic substrate for 90weeks (KotzCetal. 1968).…”

Section: Discussionmentioning

confidence: 99%

Variations in enzyme activities of Butyrivibrio fibrisolvens and Ruminococcus albus grown in continuous culture

Kotzé¹,

Kistner²

1974

Can. J. Microbiol.

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Korzc, J. P., and A. KISTNER. 1974. Variations in enzyme activities of Butyrivibriofibrisolvens and R~cminococczrs albus grown in continuous culture. Can. J. Microbiol. 20: 861-869. One strain each of Brctyrivibrio fibrisolvens and of Rurnit~ococcus albus were grown in continuous culture under glucose limitation at three dilution rates. The activities of several intermediary enzymes were determined in cell-free extracts. In both organisms, the coefficients of variation of enzyme activities were, with few exceptions, much larger than those of the dryweight concentration of cells and of glucose uptake from the medium. I n B. fibrisolvens, the coefficients of variation of all enzyme activities per milliliter of culture were much greater at a dilutioll rate ( D ) of 0.5 h-' than at D = 0.12 and 0.2 h-'. I n R. albus, on the other hand, the coefficients of variation were greater at D = 0.1 h-' than at D = 0.2 and 0.43 h-'. Cell counts made at short intervals over a period covering three doubling times of a R. albus culture growing under steady-state conditions provided no evidence of synchrony of cell divisions that might account for the fluctuations in enzyme activities. The results indicate the importance of determining the extent of variations in enzyme activities in cells growing at a particular growth rate under steady-state conditions before attempting to compare the effect of different growth rates on enzyme activities. The implications of these findings are discussed in relation to an earlier study on changes in enzyme activities in extracts of the mixed microbial population of an experimental anaerobic digester during adaptation to a synthetic substrate and after prolonged operation on this substrate. KOTZE, J. P., et A. KISTNER. 1974. Variations in enzyme activities of Blctyriuibrio fibrisolve~rs and R~rtni~~ococcra albrrs grown in continuous culture. Can. J. Microbiol. 20: 861-869. Nous avons fait croitre en culture continue sous des conditions limitantes de glucose et a trois taux de dilution, une lignee de chacun de Butyriuibrio fibrisolvet~s et Rwninococcus albrrs. Les activites de plusieurs enzymes intermediaires furent determinees dans des extraits acellulaires. Chez les deux organismes, les coefficients de variation des activites enzymatiques furent, avec

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“…In this way, adaptation is a key factor in the application of anaerobic treatment to milk processing effluents which are potentially toxic effluents (56). Several studies have supported the notion that previous adaptation of the biomass to the substrate is a beneficial or even essential condition for the well succeeded operation of anaerobic treatment systems applied to milk or fat containing effluents (94,95). Biomass adaptation allows the attainment of a higher treatment capacity (96) and acts as a defense against inhibition effects (97).…”

Section: Special Considerations For Anaerobic Treatment Of Milk Procementioning

confidence: 96%

Anaerobic Treatment of Milk Processing Wastewater

Nadais

Capela

Arroja

et al. 2010

Environmental Bioengineering

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Anaerobic processes are widely used for the treatment of milk and dairy effluents. This technology has been subjected to significant development and real-scale application in the last few decades and offers highly favorable perspectives to accomplish a complete biodegradation of the components present in milk processing wastewaters such as sugars, proteins, and fats. Nowadays, anaerobic systems for the treatment of milk wastes can be operated successfully constituting an important contribution for the preservation of environmental quality.

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Biological changes during the adaptation of an anaerobic digester to a synthetic substrate

Cited by 35 publications

References 11 publications

Influence of anaerobic culture acclimation on the degradation kinetics of various substrates

Influence of anaerobic culture acclimation on the degradation kinetics of various substrates

Variations in enzyme activities of Butyrivibrio fibrisolvens and Ruminococcus albus grown in continuous culture

Anaerobic Treatment of Milk Processing Wastewater

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