Biological characteristics and biomarkers of novel SARS-CoV-2 facilitated rapid development and implementation of diagnostic tools and surveillance measures

Ghodake, Gajanan; Shinde, Surendra K.; Kadam, Avinash A.; Saratale, Rijuta Ganesh; Syed, Asad; Elgorban, Abdallah M.; Marraiki, Najat; Kim, Dae-Young

doi:10.1016/j.bios.2021.112969

Cited by 27 publications

(22 citation statements)

References 333 publications

(309 reference statements)

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“…Thus, there is a problem with the reliability of the diagnosis. In particular, the issue of “false-negative” results, whereby positive patients are diagnosed as negative, is the main reason this method cannot be adopted as a standard diagnostic method ( Ghodake et al, 2021 ; Xu et al, 2020 ). Therefore, many researchers are striving to develop a new molecular diagnostic technology that can shorten the diagnostic time while maintaining the diagnostic accuracy of RT-PCR.…”

Section: Introductionmentioning

confidence: 99%

SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates

Dang

Park

et al. 2022

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates

Dang

Park

et al. 2022

Biosensors and Bioelectronics

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“…As no single solution preventing the spreading of viral infections exists, multiple-barrier protection mechanisms are required to block or at least slow down the speed of virus transmission [1]. Although numerous efforts to slow down the spreading of the virus have been developed, including vaccines [2], diagnostic technologies and developments in surveillance measures for SARS-CoV-2-positive patient contact tracing [3], the rapid growth of new patients has not slowed down, and additional measures are necessary to combat the pandemic crisis.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Nanohybrid Materials as Candidates for Self-Sanitizing Filters Aimed at Protection from SARS-CoV-2 in Public Areas

et al. 2022

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The COVID-19 pandemic has raised the problem of efficient, low-cost materials enabling the effective protection of people from viruses transmitted through the air or via surfaces. Nanofibers can be a great candidate for efficient air filtration due to their structure, although they cannot protect from viruses. In this work, we prepared a wide range of nanofibrous biodegradable samples containing Ag (up to 0.6 at.%) and Cu (up to 20.4 at.%) exhibiting various wettability. By adjusting the magnetron current (0.3 A) and implanter voltage (5 kV), the deposition of TiO2 and Ag+ implantation into PCL/PEO nanofibers was optimized in order to achieve implantation of Ag+ without damaging the nanofibrous structure of the PCL/PEO. The optimal conditions to implant silver were achieved for the PCL-Ti0.3-Ag-5kV sample. The coating of PCL nanofibers by a Cu layer was successfully realized by magnetron sputtering. The antiviral activity evaluated by widely used methodology involving the cultivation of VeroE6 cells was the highest for PCL-Cu and PCL-COOH, where the VeroE6 viability was 73.1 and 68.1%, respectively, which is significantly higher compared to SARS-CoV-2 samples without self-sanitizing (42.8%). Interestingly, the samples with implanted silver and TiO2 exhibited no antiviral effect. This difference between Cu and Ag containing nanofibers might be related to the different concentrations of ions released from the samples: 80 μg/L/day for Cu2+ versus 15 µg/L/day for Ag+. The high antiviral activity of PCL-Cu opens up an exciting opportunity to prepare low-cost self-sanitizing surfaces for anti-SARS-CoV-2 protection and can be essential for air filtration application and facemasks. The rough cost estimation for the production of a biodegradable nanohybrid PCL-Cu facemask revealed ~$0.28/piece, and the business case for the production of these facemasks would be highly positive, with an Internal Rate of Return of 34%.

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“…This work aimed to isolate antibodies against SARS-CoV SP from pig serum. Among four major structural proteins from the CoV genome such as SP, envelope protein, membrane protein, and NP [15], the SARS-CoV SP is a large protein with a molecular weight of 139.1 kDa, which is composed of 1,255 amino acids. The SP is classified as a group of class I viral fusion glycoproteins, and most of the protein appears on the viral surface.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Antibodies Against the Spike Protein of SARS-CoV from Pig Serum for Competitive Immunoassay

et al. 2021

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Several endemic corona viruses (eCoVs) have been reported to be the most common etiologic agents for the seasonal common cold and also cause pneumonia. These eCoVs share extensive sequence homology with SARS-CoV-2, and immune responses to eCoVs can cross-react with SARS-CoV-2 antigens. Based on such cross-reactivity of antigens among eCoVs, the IgG antibodies against the spike protein (SP) of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated from pig serum using magnetic beads immobilized with SARS-CoV SP and a protein-A column. The selectivity of the isolated antibodies was tested using different types of antigens, such as SARS-CoV-2 nucleoprotein (NP), influenza A virus (Beijing type), influenza B virus (Tokio and Florida types), human hepatitis B virus surface antigen (HBsAg), and bovine serum albumin (BSA). From the selectivity test, the anti-SP antibodies isolated from pig serum had sufficient selectivity to other kinds of viral antigens, and the apparent binding constant of the isolated antibodies was approximately 1.5 × 10 -8 M from the surface plasmon resonance (SPR) measurements. Finally, the isolated anti-SP antibodies were applied to the immunoassay of SP using competitive immunoassay configuration. The feasibility of the detection as well as the quantitative analysis of the SARS-CoV viral culture fluid was determined using four viral culture samples, namely, SARS-CoV, SARS-CoV-2, MERS-CoV, and CoV-229E.

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Biological characteristics and biomarkers of novel SARS-CoV-2 facilitated rapid development and implementation of diagnostic tools and surveillance measures

Cited by 27 publications

References 333 publications

SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates

SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates

Biodegradable Nanohybrid Materials as Candidates for Self-Sanitizing Filters Aimed at Protection from SARS-CoV-2 in Public Areas

Isolation of Antibodies Against the Spike Protein of SARS-CoV from Pig Serum for Competitive Immunoassay

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