Biological characteristics of a type I restriction-modification system in Staphylococcus aureus

Sjöström, Jan-Eric; Löfdahl, Sven; Philipson, Lennart

doi:10.1128/jb.133.3.1144-1149.1978

Cited by 13 publications

(4 citation statements)

References 29 publications

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“…Effect of temperature on bacteriophage restriction. Temporary inactivation of host restriction systems by elevated temperature has been reported in Staphylococcus aureus (30), Escherichia coli (29), and Salmonella species (34). In S. cremoris KH, inactivation of restric- a One passage of c2 phage through S. cremoris KH was carried out before plaque assay on the indicated hosts.…”

Section: Resultsmentioning

confidence: 99%

Restriction and Modification in Group N Streptococci: Effect of Heat on Development of Modified Lytic Bacteriophage

Sanders

Klaenhammer

1980

Appl Environ Microbiol

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Section: Resultsmentioning

confidence: 99%

Restriction and Modification in Group N Streptococci: Effect of Heat on Development of Modified Lytic Bacteriophage

Sanders

Klaenhammer

1980

Appl Environ Microbiol

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“…Nevertheless, it is notable that phage resistance is commonly encountered in Staph. aureus (Sjöström et al 1978;Tock and Dryden 2005), and thus, the inclusion of modified phage derivatives with expanded host ranges is worthwhile. Recently, Kelly et al (2011) quantified the incidence of phage K resistance in a bank of Irish Staph.…”

Section: Discussionmentioning

confidence: 99%

Prevention of Staphylococcus aureus biofilm formation and reduction in established biofilm density using a combination of phage K and modified derivatives

Kelly

McAuliffe

Ross

et al. 2012

Letters in Applied Microbiology

111

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Aims: To investigate the ability of a mixture of phage K and six of its modified derivatives to prevent biofilm formation by Staphylococcus aureus and also to reduce the established biofilm density. Methods and Results: The bioluminescence‐producing Staph. aureus Xen29 strain was used in the study, and incubation of this strain in static microtitre plates at 37°C for 48 h confirmed its strong biofilm‐forming capacity. Subsequently, removal of established biofilms of Staph. aureus Xen29 with the high‐titre phage combination was investigated over time periods of 24 h, 48 h and 72 h. Results suggested that these biofilms were eliminated in a time‐dependant manner, with biofilm biomass reduction significantly greater after 72 h than after 24–48 h. In addition, initial challenge of Staph. aureus Xen29 with the phage cocktail resulted in the complete inhibition of biofilm formation over a 48‐h period with no appearance of phage resistance. Conclusions: In general, our findings demonstrate the potential use of a modified phage combination for the prevention and successful treatment of Staph. aureus biofilms, which are implicated in several antibiotic‐resistant infections. Significance and Impact of the Study: This study highlights the first use of phage K for the successful removal and prevention of biofilms of Staph. aureus.

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“…Staphylococcus aureus (Sjöström et al, 1978) Salmonella (Bullas et al, 1976) Defence island system associated with restriction-modification (DISARM) Degrade phage DNA. Type of R-M system.…”

Section: Bacteriophage Exclusion (Brex)mentioning

confidence: 99%

The Human Gut Phageome: Origins and Roles in the Human Gut Microbiome

Townsend

Kelly

Muscatt

et al. 2021

Front. Cell. Infect. Microbiol.

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The investigation of the microbial populations of the human body, known as the microbiome, has led to a revolutionary field of science, and understanding of its impacts on human development and health. The majority of microbiome research to date has focussed on bacteria and other kingdoms of life, such as fungi. Trailing behind these is the interrogation of the gut viruses, specifically the phageome. Bacteriophages, viruses that infect bacterial hosts, are known to dictate the dynamics and diversity of bacterial populations in a number of ecosystems. However, the phageome of the human gut, while of apparent importance, remains an area of many unknowns. In this paper we discuss the role of bacteriophages within the human gut microbiome. We examine the methods used to study bacteriophage populations, how this evolved over time and what we now understand about the phageome. We review the phageome development in infancy, and factors that may influence phage populations in adult life. The role and action of the phageome is then discussed at both a biological-level, and in the broader context of human health and disease.

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Biological characteristics of a type I restriction-modification system in Staphylococcus aureus

Cited by 13 publications

References 29 publications

Restriction and Modification in Group N Streptococci: Effect of Heat on Development of Modified Lytic Bacteriophage

Restriction and Modification in Group N Streptococci: Effect of Heat on Development of Modified Lytic Bacteriophage

Prevention of Staphylococcus aureus biofilm formation and reduction in established biofilm density using a combination of phage K and modified derivatives

The Human Gut Phageome: Origins and Roles in the Human Gut Microbiome

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