Biological Characteristics of Human Placental Mesenchymal Stem Cells and Their Proliferative Response to Various Cytokines

Liu, Dong; Wang, Guoyun; Dong, Bo; Zhang, Yue-Cun; Wang, Yuanxiu; Sun, Bing-Cui

doi:10.1159/000105674

Cited by 37 publications

(34 citation statements)

References 83 publications

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“…The characteristics of the placenta-derived stem cells (PDSCs) are those of MSCs exhibiting immunomodulatory properties [68][69][70] with the additional inducible expression of pluripotency markers SSEA-4, Nanog 3, Oct-4 [71,72] and Rex-1 [72]. They display the typical wide range in differentiation capacity spectrum of MSCs [62,[71][72][73][74], including differentiation into neuronal and glial cells in vitro [75], insulin-positive cells both in vitro and in vivo [76], hepatocytes [77] and generation of heart valves when seeded in biodegradable scaffolds [78].…”

Section: Placenta Stem Cellsmentioning

confidence: 99%

Novel Sources of Fetal Stem Cells: Where do they Fit on the Developmental Continuum?

2009

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The recent isolation of fetal stem cells from several sources either at the early stages of development or during the later trimesters of gestation, sharing similar growth kinetics and expressing pluripotency markers, provides strong support to the notion that these cells may be biologically closer to embryonic stem cells, actually representing intermediates between embryonic stem cells and adult mesenchymal stem cells, regarding proliferation rates and plasticity features, and thus able to confer an advantage over postnatal mesenchymal stem cells derived from conventional adult sources such as bone marrow. This conclusion has been strengthened by the different pattern of growth potential between the two stage-specific types of sources, as assessed by transcriptomic and proteomic analysis. A series of recent studies regarding the numerous novel features of fetal stem cells has reignited our interest in the field of stem-cell biology and in the possibilities for the eventual repair of damaged organs and the generation of in vitro tissues on biomimetic scaffolds for transplantation. These studies, employing elegant approaches and novel technologies, have provided new insights regarding the nature and the potential of fetal stem cells derived from placenta, amniotic fluid, amnion or umbilical cord. In this update, we highlight the major progression that has occurred in fetal stem-cell biology and discuss the most important areas for future investigation in the field of regenerative medicine.

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Section: Placenta Stem Cellsmentioning

confidence: 99%

Novel Sources of Fetal Stem Cells: Where do they Fit on the Developmental Continuum?

2009

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“…PMSCs can be differentiated in vitro under specific stimulatory environments into derivatives of the mesenchymal cell lineage such as osteocytes, adipocytes, myocytes and chondrocytes [1,[3][4][5][6][7]. In addition, there is evidence of differentiation in vitro into cell types characteristic of other lineages such as hepatocyte-like cells and neural-like cells [3,4,[7][8][9][10][11], but in vivo evidence for such differentiation is very limited.…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche

et al. 2010

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Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche Castrechini, N. M.; Murthi, P.; Gude, N. M.; Erwich, Jan Jaap H. M.; Gronthos, S.; Zannettino, A.; Brennecke, S. R.; Kalionis, B.; Brennecke, S.P. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. t r a c tThe chorionic villi of human term placentae are a rich source of mesenchymal stem cells (PMSCs). The stem cell ''niche'' within the chorionic villi regulates how PMSCs participate in placental tissue generation, maintenance and repair, but the anatomic location of the niche has not been defined. A number of cell surface markers for phenotypic characterisation of mesenchymal stem cells (MSCs) were employed to identify the stem cell niche within the chorionic villi of first trimester and term human placenta. This included antibodies to pericyte cell surface markers STRO-1 and 3G5, which have been used to identify mesenchymal stem cells in other tissues, but have not been studied in placental tissues. PMSCs were isolated from term human placentae and shown to have stem cell properties by their ability to grow on untreated plastic culture ware, capacity for forming clones (i.e. clonogenicity) and their capability to differentiate into adipocytes, chondrocytes and osteocytes. Western analysis confirmed that STRO-1 and 3G5 are present in placental protein extracts and in PMSCs. Immunocytochemistry revealed PMSCs were positive for MSC cell surface markers (STRO-1, 3G5, CD105, CD106, CD146, CD49a, a-SMA) and negative for haematopoietic stem cell markers (CD117, CD34) and endothelial markers (CD34, vWF). Immunohistochemistry with antibodies to MSC cell surface markers on first trimester and term tissues revealed a vascular niche for PMSCs. Dual-label immunofluorescence analysis was used to compare STRO-1 antibody staining with that of endothelial cell marker vWF and found no significant overlap in staining. This indicated that some PMSCs have a pericyte-like phenotype. We propose that the vascular niche harbours a pool of PMSCs that can give rise to committed progenitors for tissue maintenance and repair, and that PMSCs contribute to vessel maturation and stabilization.

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“…Currently it is speculated that MSC migrate to sites of injury where biological effects relate to the local production of growth factors rather than to engraftment and tissue repair by differentiation [54]. Indeed MSC have been shown to respond differentially to pro-or anti-inflammatory cytokines: whereas proinflammatory cytokines like interleukin 1 or 6 stimulated, interleukin 4 or hepatocyte growth factor decelerated proliferation [139]. Next, MSC secrete a variety of trophic factors exerting both paracrine and autocrine effects.…”

Section: Regenerative Medicinementioning

confidence: 99%

Mesenchymal Stromal Cells from Umbilical Cord Blood

Bieback¹,

Klüter

2007

CSCR

138

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Mesenchymal Stromal Cells (MSC) are key candidates for cellular therapies. Although most therapeutic applications have focused on adult bone marrow derived MSC, increasing evidence suggests that MSC are present within a wide range of tissues. Umbilical cord blood (CB) has been proven to be a valuable source of hematopoietic stem cells, but its therapeutic potential extends beyond the hematopoietic component suggesting regenerative potential in solid organs as well. There is evidence that other stem or progenitor populations, such as MSC, exist in CB which might be responsible for these effects. Many different stem and progenitor cell populations have been postulated with potential ranging from embryonic like to lineage-committed progenitor cells. Based on the confusing data, this review focuses on a human CB derived, plastic adherent fibroblastoid population expressing similar characteristics to bone marrow derived MSC. It concentrates especially on concepts of isolation and expansion, comparing the phenotype with bone marrow derived MSC, describing the differentiation capacity and finally in the last the therapeutic potential with regard to regenerative medicine, stromal support, immune modulation and gene therapy.

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Biological Characteristics of Human Placental Mesenchymal Stem Cells and Their Proliferative Response to Various Cytokines

Cited by 37 publications

References 83 publications

Novel Sources of Fetal Stem Cells: Where do they Fit on the Developmental Continuum?

Novel Sources of Fetal Stem Cells: Where do they Fit on the Developmental Continuum?

Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche

Mesenchymal Stromal Cells from Umbilical Cord Blood

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