Biological characterization of EDC-crosslinked collagen–hyaluronic acid matrix in dermal tissue restoration

Park, Si Nae; Lee, Hye Jung; Lee, Kwang Hoon; Suh, Hwal

doi:10.1016/s0142-9612(02)00550-1

Cited by 223 publications

(127 citation statements)

References 24 publications

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“…34,35 In this study each value of swelling test was averaged from three parallel measurements. The results of the water uptake studies are displayed in Figure 3A & 3B.…”

Section: Swelling Evaluationmentioning

confidence: 99%

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Preparation and Physicochemical Characterization of Nano- Hydroxyapatite Based 3D Porous Scaffold for Biomedical Application

Sultana

Rana

Akhtar

et al. 2017

ATROA

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Over the last decades, hydroxyapatite (HA) and its composite with biopolymer have been extensively developed and applied in biomedical application. The aim of this study is to produce a novel 3D porous HA scaffolds for bone tissue regeneration. Scaffolds with varying composition of HA, chitosan, gelatin, agarose and poly vinyl alcohol (PVA) were prepared using freeze drying method. The composite scaffold was analyzed to determine density, porosity, biodegradability, swelling kinetics, morphology and structural properties. Porosity and density of the prepared scaffolds were 85.17 to 92.21% and 0.317 to 0.495g/cm3, respectively. The swelling ability of the prepared scaffolds showed similar efficacy. FTIR analysis showed intermolecular interaction between components in the scaffold. Pore size of the developed scaffolds were measured by scanning electron microscopy and found that regardless of their composition showed adequate pore sizes ranging from 174 to 405µm. Brine shrimp lethality assay indicated that the obtained scaffolds had no cytotoxic effects, and that they had good biocompatibility. The results suggested that, these homogeneous composite scaffolds were found to be potential candidates as bone grafting materials for bone tissue engineering applications. On the other hand, polyvinyl alcohol, PVA, is a water soluble synthetic resin which is obtained through polymerization of vinyl acetate monomer. By hydrolysis, the acetate groups are converted in hydroxyl groups. The degree of hydrolysis in a polyvinyl alcohol reagent is controlled by this process. The polar nature of poly (vinyl alcohol) facilitates the formation of hydrogen bonds and eventual condensation with silanol groups (from developing polysilicate network) formed by hydrolysis of the silicon alkoxides. Keywords17 Moreover PVA has been proposed for controlled release systems and is employed in a variety of biomedical applications, generally being considered to be biocompatible. 18,19 A polymer or combination of polymers can be fabricated into a porous scaffold through various techniques, such as sintering, salt leaching, freeze-drying, solvent casting, gas foaming, fibre meshes, phase separation, melt moulding, emulsion free drying, solution casting, etc. Sintering uses high temperatures to bond substances together as well as burn out organic material to form a porous structure.20 Salt leaching is also feasible, but is limited by the prolonged contact of particles with water and the requirement for salt removal.21 This study has chosen freeze-drying because it can form highly a porous structure and offer stability and ease of handling. 22,23 This study focuses on preparing and characterizing composite scaffold synthesized from three natural-based materials-chitosan, gelatin and HA. Afterwards in combination with agarose/PVA, these three materials offer potential synergies between physical properties and bioactivity for use as bone substitutes in bone grafts, benefiting a range of surgical applications. So a well-designed three-dimensional ...

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“…34,35 In this study each value of swelling test was averaged from three parallel measurements. The results of the water uptake studies are displayed in Figure 3A & 3B.…”

Section: Swelling Evaluationmentioning

confidence: 99%

“…26,33 Scaffold swelling properties have been shown to significantly influence cell behaviors such as adhesion, growth and differentiation. 34,35 In this study each value of swelling test was averaged from three parallel measurements. The results of the water uptake studies are displayed in Figure 3A & 3B.…”

Section: Swelling Evaluationmentioning

confidence: 99%

Preparation and Physicochemical Characterization of Nano- Hydroxyapatite Based 3D Porous Scaffold for Biomedical Application

Sultana

Rana

Akhtar

et al. 2017

ATROA

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“…[7,27] Collagen sheets act as a mechanical support, reduce oedema and loss of fluids from the wound site, facilitate the of migration of fibroblasts into the wound, thus enhancing the metabolic activity of the granulation tissue. [27][28][29] This fact is of particular importance in treating painful and bleeding ulcers of the mucosal surfaces of the body such as buccal cavity, nasal cavity, conjunctiva, urethra, vagina, and anal canal.…”

Section: Collagen As a Biomaterials In Plastic And Reconstructive Surgerymentioning

confidence: 99%

Collagen membrane for reconstruction of soft tissue defects after surgery of oral cancer and precancer: a brief review

Vastani¹,

Chourasia²,

Pahlajani³

et al. 2016

PAR

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There are some surgeries after which a temporary cover for raw wounds is required to ensure healing. Some of those circumstances are loss of tissue due to burns, trauma, amputation, chronic ulcer, leprosy, and skin graft sites. Although the body initiates regeneration mechanisms, however the time taken for complete healing of wounds is unpredictable. Also, there is a tendency for long standing wounds to undergo infection and scarring. Oral mucosa is no exception to scarring and infection of wounds and there has always been a search for new materials that can be used for coverage of oral defects. Xenogenous collagen is one such grafting material. Over the years collagen implant solutions for a number of clinical applications include general surgery, burn surgery, neurosurgery, plastic and reconstructive surgery, oral surgery, and peripheral nerve and tendon surgery. This paper aims to focus on collagen as an effective option of wound closure in plastic and reconstructive surgery of the head and neck, especially after loss of soft tissue following resection of oral malignancies.

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“…These biological dressings create a physiological interface between the wound surface and the environment that is impermeable to bacteria, thus helping to prevent local wound infection. 25 In addition, the collagen sheets are noninflammatory, facilitate fibroblast migration to the wound site, assist in extracellular matrix synthesis, are nontoxic, and minimize scarring. 13 Conformant (Smith & Nephew, Inc, Largo, FL, USA) is a nonadherent contact layer that comes in rolls and can be applied to large involved areas outside the operating room.…”

Section: Wound Managementmentioning

confidence: 99%

Drug-Hypersensitivity Syndrome: Diagnosis and Treatment

Hamm¹

2011

Journal of the American College of Clinical Wound Specialists

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Biological characterization of EDC-crosslinked collagen–hyaluronic acid matrix in dermal tissue restoration

Cited by 223 publications

References 24 publications

Preparation and Physicochemical Characterization of Nano- Hydroxyapatite Based 3D Porous Scaffold for Biomedical Application

Preparation and Physicochemical Characterization of Nano- Hydroxyapatite Based 3D Porous Scaffold for Biomedical Application

Collagen membrane for reconstruction of soft tissue defects after surgery of oral cancer and precancer: a brief review

Drug-Hypersensitivity Syndrome: Diagnosis and Treatment

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