Biological control of peanut southern blight (<i>Sclerotium rolfsii</i>) by the strain <i>Bacillus pumilus</i> LX11

Xu, Manlin; Zhang, Xia; Yu, Jing; Wu, Juxiang; Li, Xinguo; Chi, Yucheng; Wan, Shungang

doi:10.1080/09583157.2020.1725441

Cited by 17 publications

(12 citation statements)

References 8 publications

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“…Our results show the high potential of B. velezensis strain GA1 to protect peanut from disease caused by A. rolfsii , an economically important pathogen causing stem rot. Other Bacillus isolates belonging to various species of the subtilis subgroup have also been reported as strong antagonists of this phytopathogen in vitro ( Ahmad et al., 2019 ; Chen et al., 2020 ; Xu et al., 2020 ; Kumari et al., 2021 ). However, studies demonstrating biocontrol of this disease under greenhouse or field conditions are rare ( Tonelli et al., 2011 ; Figueredo et al., 2017 ) and the mechanisms underlying this protective effect are poorly documented even if some involvement of lipopeptides and volatiles were evoked based on the potential of the tested strains to form these compounds in vitro ( Munjal et al., 2016 ; Agisha et al., 2019 ; Chen et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Bacillus lipopeptide-mediated biocontrol of peanut stem rot caused by Athelia rolfsii

et al. 2023

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IntroductionPeanut (Arachis hypogaea L.) is a widespread oilseed crop of high agricultural importance in tropical and subtropical areas. It plays a major role in the food supply in the Democratic Republic of Congo (DRC). However, one major constraint in the production of this plant is the stem rot (white mold or southern blight) disease caused by Athelia rolfsii which is so far controlled mainly using chemicals. Considering the harmful effect of chemical pesticides, the implementation of eco-friendly alternatives such as biological control is required for disease management in a more sustainable agriculture in the DRC as in the other developing countries concerned. Bacillus velezensis is among the rhizobacteria best described for its plant protective effect notably due to the production of a wide range of bioactive secondary metabolites. In this work, we wanted to evaluate the potential of B. velezensis strain GA1 at reducing A. rolfsii infection and to unravel the molecular basis of the protective effect.Results and discussionUpon growth under the nutritional conditions dictated by peanut root exudation, the bacterium efficiently produces the three types of lipopeptides surfactin, iturin and fengycin known for their antagonistic activities against a wide range of fungal phytopathogens. By testing a range of GA1 mutants specifically repressed in the production of those metabolites, we point out an important role for iturin and another unidentified compound in the antagonistic activity against the pathogen. Biocontrol experiments performed in greenhouse further revealed the efficacy of B. velezensis to reduce peanut disease caused by A. rolfsii both via direct antagonism against the fungus and by stimulating systemic resistance in the host plant. As treatment with pure surfactin yielded a similar level of protection, we postulate that this lipopeptide acts as main elicitor of peanut resistance against A. rolfsii infection.

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Section: Discussionmentioning

confidence: 99%

Bacillus lipopeptide-mediated biocontrol of peanut stem rot caused by Athelia rolfsii

et al. 2023

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“…Abo-Zaid et al [34] used a talc-based medium to ferment Streptomyces cellulosae Actino 48 with a high chitinase production which could effectively inhibit the mycelial growth of Sclerotium rolfsii. The rhizosphere growth-promoting bacteria Bacillus pumilus LX11 [14] and the seed endophytes Bacillus velezensis LHSB1 [35] can effectively antagonize Sclerotium rolfsii to signi cantly reduce the incidence of stem rot and can be used as potential biocontrol agents. The present research found that the high concentration fermentation solution of Novosphingobium panipatense, a dominant strain signi cantly enriched by calcium at the pod lling stage, could signi cantly inhibit the mycelial growth and nucleus formation of Sclerotium rolfsii.…”

Section: Discussionmentioning

confidence: 99%

“…usually cause southern blight, which could form white lamentous hyphae at the infected site with the sclerotia formed in the later growth stage. Subsequently, the leaves turned yellow or brown to wither, and then the plants wilted and died [14]. Peanut leaf spots include the brown spot (Cercospora arachidicola), the black spot (Cercosporidium personatum), the scorch spot (Leptosphaerulina arachidicola), the web blotch (Phoma arachidicola) and so on.…”

Section: Introductionmentioning

confidence: 99%

The Resistance of Peanut to Soil-Borne Pathogens Improved by Rhizosphere Probiotics Under Calcium Treatment

Zhang

Lin

et al. 2021

Preprint

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Background: Peanut (Arachis hypogaea L.) is an important oil and economic crop. Calcium can regulate plant autoimmunity, respond to biotic and abiotic stresses, and improve plant resistance to pathogens. The enrichment of beneficial microorganisms in the rhizosphere is related to plant disease resistance and soil development. The purpose was to analyze the differences of peanut rhizosphere microbial community structure between calcium treatment and control during two growth stages, and the reason that calcium application could improve peanut’s resistance to soil-borne pathogens.. Results: The 16S amplicon sequencing of rhizosphere microbiome showed that calcium application significantly enriched Serratia marcescens and other three dominant strains at the seedling stage. At the pod filling stage, ten dominant stains such as Sphingomonas changbaiensis and Novosphingobium panipatense were enriched by calcium. Serratia marcescens aseptic fermentation filtrate was mixed with PDA medium and inoculated with soil-borne pathogens, which could inhibit the growth of Fusarium solani and Aspergillus flavus at the seedling stage. The fermentation filtrate of Novosphingobium panipatense was mixed with PDA medium and inoculated with main pathogens, which could inhibit the growth of Sclerotium rolfsii and Leptosphaerulina arachidicola.Conclusions: Calcium application enhanced the ability of peanuts to resist pathogens by enriching specific dominant bacteria.

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“…For example, B. velezensis and B. siamensis can inhibit the occurrence of peanut S. rolfsii Sacc. by producing protease, cellulase, amylase and ferroportin, and reduce the peanut disease index ( Xu et al, 2020 ). B. amylotica LX-J1 and 41B-1 can colonize peanut roots and induce defense responses in peanut plants, root irrigation treatment showed significant resistance to S. rolfsii .…”

Section: Introductionmentioning

confidence: 99%

Study on the mechanism of peanut resistance to Fusarium oxysporum infection induced by Bacillus thuringiensis TG5

Du,

2024

Front. Microbiol.

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Peanut root rot, commonly referred to as rat tail or root rot, is caused by a range of Fusarium species. A strain of bacteria (named TG5) was isolated from crop rhizosphere soil in Mount Taishan, Shandong Province, China, through whole genome sequencing that TG5 was identified as Bacillus thuringiensis, which can specifically produce chloramphenicol, bacitracin, clarithromycin, lichen VK21A1 and bacitracin, with good biological control potential. Based on liquid chromatography tandem mass spectrometry metabonomics analysis and transcriptome conjoint analysis, the mechanism of TG5 and carbendazim inducing peanut plants to resist F. oxysporum stress was studied. In general, for peanut root rot caused by F. oxysporum, B. thuringiensis TG5 has greater advantages than carbendazim and is environmentally friendly. These findings provide new insights for peanut crop genetics and breeding, and for microbial pesticides to replace traditional highly toxic and highly polluting chemical pesticides. Based on the current background of agricultural green cycle and sustainable development, it has significant practical significance and broad application prospects.

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Biological control of peanut southern blight (Sclerotium rolfsii) by the strain Bacillus pumilus LX11

Cited by 17 publications

References 8 publications

Bacillus lipopeptide-mediated biocontrol of peanut stem rot caused by Athelia rolfsii

Bacillus lipopeptide-mediated biocontrol of peanut stem rot caused by Athelia rolfsii

The Resistance of Peanut to Soil-Borne Pathogens Improved by Rhizosphere Probiotics Under Calcium Treatment

Study on the mechanism of peanut resistance to Fusarium oxysporum infection induced by Bacillus thuringiensis TG5

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