Biological Control of Potato Tuber Soft Rot using &lt;i&gt;N&lt;/i&gt;-Acyl-L-Homoserine Lactone-Degrading Endophytic Bacteria

Hà, Nguyễn Thanh; Minh, Tran Quang; Hoi, Pham Xuan; Thủy, Nguyễn Thị Thanh; Furuya, Naruto; Long, Hoang Hoa

doi:10.18520/cs/v115/i10/1921-1927

Cited by 17 publications

(5 citation statements)

References 35 publications

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“…Variovorax can facilitate the interaction between plants and microorganisms by manipulating plant hormone levels to balance the growth of plant roots. V. paradoxus can significantly inhibit potato tuber soft rot and is considered to be a new type of potato tuber soft rot biological control agent ( Ha et al, 2018 ). In our study, the abundance of V. paradoxus in healthy root endophyte samples was high.…”

Section: Discussionmentioning

confidence: 99%

Structure and Function of Rhizosphere Soil and Root Endophytic Microbial Communities Associated With Root Rot of Panax notoginseng

et al. 2022

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Panax notoginseng (Burk.) F. H. Chen is a Chinese medicinal plant of the Araliaceae family used for the treatment of cardiovascular and cerebrovascular diseases in Asia. P. notoginseng is vulnerable to root rot disease, which reduces the yield of P. notoginseng. In this study, we analyzed the rhizosphere soil and root endophyte microbial communities of P. notoginseng from different geographical locations using high-throughput sequencing. Our results revealed that the P. notoginseng rhizosphere soil microbial community was more diverse than the root endophyte community. Rhodopseudomonas, Actinoplanes, Burkholderia, and Variovorax paradoxus can help P. notoginseng resist the invasion of root rot disease. Ilyonectria mors-panacis, Pseudomonas fluorescens, and Pseudopyrenochaeta lycopersici are pathogenic bacteria of P. notoginseng. The upregulation of amino acid transport and metabolism in the soil would help to resist pathogens and improve the resistance of P. notoginseng. The ABC transporter and gene modulating resistance genes can improve the disease resistance of P. notoginseng, and the increase in the number of GTs (glycosyltransferases) and GHs (glycoside hydrolases) families may be a molecular manifestation of P. notoginseng root rot. In addition, the complete genomes of two Flavobacteriaceae species and one Bacteroides species were obtained. This study demonstrated the microbial and functional diversity in the rhizosphere and root microbial community of P. notoginseng and provided useful information for a better understanding of the microbial community in P. notoginseng root rot. Our results provide insights into the molecular mechanism underlying P. notoginseng root rot and other plant rhizosphere microbial communities.

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Section: Discussionmentioning

confidence: 99%

Structure and Function of Rhizosphere Soil and Root Endophytic Microbial Communities Associated With Root Rot of Panax notoginseng

et al. 2022

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“…Among these, the use of QQ bacteria or QQ enzymes has appeared as an efficient tool for managing plant diseases ( Uroz et al, 2009 ). Several QQ strains such as Cupriavidus sp., A. lactucae , Burkholderia anthina , O. intermedium , Bacillus sp., Variovorax sp., Variovorax paradoxus , and A. tumefaciens have been discovered and applied as tools for the biocontrol of plant diseases ( Ha et al, 2018 ; Ye et al, 2019 , 2020b , c ; Fan et al, 2020 ). The expression of QQ hydrolytic enzymes (AttM and AiiA) quenched virulence in the pathogenic bacteria Dickeya spp.…”

Section: Discussionmentioning

confidence: 99%

Exploration of the Quorum-Quenching Mechanism in Pseudomonas nitroreducens W-7 and Its Potential to Attenuate the Virulence of Dickeya zeae EC1

Zhang

Fan

et al. 2021

Front. Microbiol.

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Quorum quenching (QQ) is a novel, promising strategy that opens up a new perspective for controlling quorum-sensing (QS)-mediated bacterial pathogens. QQ is performed by interfering with population-sensing systems, such as by the inhibition of signal synthesis, catalysis of degrading enzymes, and modification of signals. In many Gram-negative pathogenic bacteria, a class of chemically conserved signaling molecules named N-acyl homoserine lactones (AHLs) have been widely studied. AHLs are involved in the modulation of virulence factors in various bacterial pathogens including Dickeya zeae. Dickeya zeae is the causal agent of plant-rot disease of bananas, rice, maize, potatoes, etc., causing enormous economic losses of crops. In this study, a highly efficient AHL-degrading bacterial strain W-7 was isolated from activated-sludge samples and identified as Pseudomonas nitroreducens. Strain W-7 revealed a superior ability to degrade N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) and completely degraded 0.2 mmol/L of OdDHL within 48 h. Gas chromatography-mass spectrometry (GC-MS) identified N-cyclohexyl-propanamide as the main intermediate metabolite during AHL biodegradation. A metabolic pathway for AHL in strain W-7 was proposed based on the chemical structure of AHL and intermediate products. In addition to the degradation of OdDHL, this strain was also found to be capable of degrading a wide range of AHLs including N-(3-oxohexanoyl)-l-homoserine lactone (OHHL), N-(3-oxooctanoyl)-l-homoserine lactone (OOHL), and N-hexanoyl-l-homoserine lactone (HHL). Moreover, the application of strain W-7 as a biocontrol agent could substantially attenuate the soft rot caused by D. zeae EC1 to suppress tissue maceration in various host plants. Similarly, the application of crude enzymes of strain W-7 significantly reduced the disease incidence and severity in host plants. These original findings unveil the biochemical aspects of a highly efficient AHL-degrading bacterial isolate and provide useful agents that exhibit great potential for the control of infectious diseases caused by AHL-dependent bacterial pathogens.

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“…The natural interactions between soil microorganisms, which may impact both pathogens and their hosts, can be considered to protect plants from soft rot [175]. There is an increasing emphasis on research dedicated to the discovery of new strains of bacteria active against soft rot disease, including, but not limited to, the genera Agrobacterium [176], Bacillus [160,[176][177][178][179][180][181][182][183][184][185][186][187], Bdellovibrio [188], Brevibacillus [185], Lactobacillus [177], Lellilottia [189], Ochrobactrum [190,191], Paenibacillus [192], Pseudomonas [178,179,183,184,186,[193][194][195][196], Rahnella [189], Rhodococcus [197][198][199], Serratia [110,189,200], Streptomyces [178,201,202], and Variovorax [176]. There are several reports of biocontrol strains against SRP of fungi: Aspergillus [203], Penicillium…”

Section: Pathogen Controlmentioning

confidence: 99%

“…Moreover, it has been suggested that the mixture of different types of AHLs is the most prominent method of induction of plant defenses [229]. Additionally, many biological control agents use quorum quenching (disruption of quorum sensing) as their mode of action against soft rot disease, e.g., Agrobacterium [176], Bacillus [176], Ochrobactrum [190,191], Rhodococcus [197][198][199], and Variovorax [176].…”

Section: Pathogen Controlmentioning

confidence: 99%

Looking for Resistance to Soft Rot Disease of Potatoes Facing Environmental Hypoxia

Maciag,

Kozieł,

Otulak-Kozieł

et al. 2024

IJMS

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Plants are exposed to various stressors, including pathogens, requiring specific environmental conditions to provoke/induce plant disease. This phenomenon is called the “disease triangle” and is directly connected with a particular plant–pathogen interaction. Only a virulent pathogen interacting with a susceptible plant cultivar will lead to disease under specific environmental conditions. This may seem difficult to accomplish, but soft rot Pectobacteriaceae (SRPs) is a group virulent of pathogenic bacteria with a broad host range. Additionally, waterlogging (and, resulting from it, hypoxia), which is becoming a frequent problem in farming, is a favoring condition for this group of pathogens. Waterlogging by itself is an important source of abiotic stress for plants due to lowered gas exchange. Therefore, plants have evolved an ethylene-based system for hypoxia sensing. Plant response is coordinated by hormonal changes which induce metabolic and physiological adjustment to the environmental conditions. Wetland species such as rice (Oryza sativa L.), and bittersweet nightshade (Solanum dulcamara L.) have developed adaptations enabling them to withstand prolonged periods of decreased oxygen availability. On the other hand, potato (Solanum tuberosum L.), although able to sense and response to hypoxia, is sensitive to this environmental stress. This situation is exploited by SRPs which in response to hypoxia induce the production of virulence factors with the use of cyclic diguanylate (c-di-GMP). Potato tubers in turn reduce their defenses to preserve energy to prevent the negative effects of reactive oxygen species and acidification, making them prone to soft rot disease. To reduce the losses caused by the soft rot disease we need sensitive and reliable methods for the detection of the pathogens, to isolate infected plant material. However, due to the high prevalence of SRPs in the environment, we also need to create new potato varieties more resistant to the disease. To reach that goal, we can look to wild potatoes and other Solanum species for mechanisms of resistance to waterlogging. Potato resistance can also be aided by beneficial microorganisms which can induce the plant’s natural defenses to bacterial infections but also waterlogging. However, most of the known plant-beneficial microorganisms suffer from hypoxia and can be outcompeted by plant pathogens. Therefore, it is important to look for microorganisms that can withstand hypoxia or alleviate its effects on the plant, e.g., by improving soil structure. Therefore, this review aims to present crucial elements of potato response to hypoxia and SRP infection and future outlooks for the prevention of soft rot disease considering the influence of environmental conditions.

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Biological Control of Potato Tuber Soft Rot using <i>N</i>-Acyl-L-Homoserine Lactone-Degrading Endophytic Bacteria

Cited by 17 publications

References 35 publications

Structure and Function of Rhizosphere Soil and Root Endophytic Microbial Communities Associated With Root Rot of Panax notoginseng

Structure and Function of Rhizosphere Soil and Root Endophytic Microbial Communities Associated With Root Rot of Panax notoginseng

Exploration of the Quorum-Quenching Mechanism in Pseudomonas nitroreducens W-7 and Its Potential to Attenuate the Virulence of Dickeya zeae EC1

Looking for Resistance to Soft Rot Disease of Potatoes Facing Environmental Hypoxia

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