Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 10 3 -10 4 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.
Nonlinear buckling analysis for honeycomb auxetic-core sandwich toroidal shell segments with CNT-reinforced face sheets surrounded by elastic foundations under the radial pressure is presented in this study. The basic equation system of shells is established based on the von Kármán–Donnell nonlinear shell theory, combined with Stein and McElman approximation. Meanwhile, the foundation-shell elastic interaction is simulated by the foundation model based on the Pasternak assumption. The Galerkin procedure is utilized to achieve the pre-buckling and post-buckling responses for the shell, from which the radially critical buckling load is determined. Numerical analysis shows the various influences of auxetic-core layer, CNT-reinforced face sheets, and elastic foundation on the pre-buckling and postbuckling behavior of sandwich shells with CNT reinforced face sheets.
Auxetic materials are usually designed as cores for structures subject to high impulse loads. Furthermore, the lightweight and high load capacity of the auxetic core construction is also an important advantage even for structures subjected to static loads. The combination of auxetic core and face sheets made by the advanced composite materials is a solution to dramatically increase the load-carrying capacity of the structure. In this paper, a new design of auxetic-core cylindrical shells with carbon nanotube-reinforced coatings is presented. Additionally, the nonlinear buckling behaviors of auxetic-core cylindrical shells with carbon nanotube-reinforced coatings under axially compressive loads are investigated. Three distributed types of functionally graded carbon nanotube-reinforced coatings and the honeycomb lattice form of the auxetic core are investigated. The homogenization model for auxetic lattice structures is considered to constitute the formulations of stiffnesses of the core layer. The nonlinear basic formulations are formulated by using the geometrically nonlinear Donnell shell theory considering Pasternak’s foundation. The Galerkin procedure can be applied three times for three states of buckling behaviors, and the expressions of the compressive load-maximal deflection and compressive load-average end shortening postbuckling curves are achieved. The numerically obtained investigations present the significant effects of auxetic core, volume fraction, direction arrangement and distributed law of carbon nanotube, foundation stiffnesses, geometrical parameters of auxetic core and shell on the critical buckling load and postbuckling behavior of structures.
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