Biological control of Sclerotium fruit rot of snake fruit and stem rot of lettuce by Trichoderma sp. T76-12/2 and the mechanisms involved

Wonglom, Prisana; Daengsuwan, Wilailuck; S, Itó; Sunpapao, Anurag

doi:10.1016/j.pmpp.2019.04.007

Cited by 67 publications

(36 citation statements)

References 21 publications

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“…: Fr., and S. sclerotiorum (Lib.) de Bary compared with previous studies and have a potential application in biological control [ 31 , 32 , 33 ].…”

Section: Discussionmentioning

confidence: 97%

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Shi

et al. 2020

IJMS

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The plant Chloranthus japonicus Sieb is known for its anticancer properties and mainly distributed in China, Japan, and Korea. In this study, we firstly investigated the diversity and antimicrobial activity of the culturable endophytic fungi from C. japonicus. A total of 332 fungal colonies were successfully isolated from 555 tissue segments of the medicinal plant C. japonicus collected from Qinling Mountains, China. One hundred and thirty representative morphotype strains were identified according to ITS rDNA sequence analyses and were grouped into three phyla (Ascomycota, Basidiomycota, Mucoromycota), five classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Agaricomycetes, Mucoromycetes), and at least 30 genera. Colletotrichum (RA, 60.54%) was the most abundant genus, followed by Aspergillus (RA, 11.75%) and Diaporthe (RA, 9.34%). The Species Richness Index (S, 56) and the Shannon-Wiener Index (H′, 2.7076) indicated that C. japonicus harbored abundant fungal resources. Thirteen out of 130 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. Among of these, F8158, which was identified as Trichoderma cf. harzianum, exhibited good antagonistic capacities (the percent inhibition of mycelial growth ranged from 47.72~88.18) for different pathogens and has a potential application in biological control. In addition, it is noteworthy that the strain F8157 (Thanatephorus cucumeris, an opportunistic pathogen) showed antibacterial and antifungal activity, which is reported firstly in this study, and should be investigated further. Taken together, these results indicated that the endophytic fungi from C. japonicus may be of potential interest in screening bio-control agents and discovering of new bioactive compounds.

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“…: Fr., and S. sclerotiorum (Lib.) de Bary compared with previous studies and have a potential application in biological control [ 31 , 32 , 33 ].…”

Section: Discussionmentioning

confidence: 97%

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Shi

et al. 2020

IJMS

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“…They have been used in the production of enzymes and biological controls of plant diseases (Samuels 1996). Trichoderma species excrete enzymes such as chitinases, β-glucanases and proteases; these enzymes are induced during interactions between Trichoderma and cell walls of phytopathogenic fungi (Wonglom et al 2019;Baiyee et al 2019b). Although other enzymes may be also involved in the complete degradation of cell walls of phytopathogenic fungi, chitinase is generally considered an important enzyme since its substrate, chitin, is the most abundant component in cell walls of many fungus species (Baek et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Characterisation and antifungal activity of extracellular chitinase from a biocontrol fungus, Trichoderma asperellum PQ34

et al. 2019

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Trichoderma species were known as biological control agents against phytopathogenic fungi because they produce a variety of chitinases. Chitinases are hydrolytic enzymes that break down glycosidic bonds in chitin, a major component of the cell walls of fungi. The present study shows that extracellular chitinase activity reached a maximum value of approximately 22 U/mL after 96 h of T. asperellum PQ34 strain culture. The optimal temperature and pH of enzyme are 40°C and 7, respectively, whereas the thermal and pH stability range from 25°C to 50°C and 4 to 10, respectively. Chitinase at 60 U/mL inhibited nearly completely in vitro growth of Colletotrichum sp. (about 95%) and Sclerotium rolfsii (about 97%). In peanut plants, 20 U/mL of chitinase significantly reduced the incidence of S. rolfsii infection compared to controls. The fungal infection incidence of seeds before germination and 30 days after germination was only 2.22% and 2.38%, while the control was 13.33% and 17.95%. Besides, chitinase from T. asperellum PQ34 can also prevent anthracnose that is caused by Colletotrichum sp. on both mango and chilli fruits up to 72 h after enzyme pretreatment at 40 U/mL. In mango and chilli fruits infected with anthracnose, 40 U/mL dose of chitinase inhibited the growth of fungi after 96 h of treatment, the diameter of lesion was only 0.88 cm for mango and 1.45 cm for chilli, while the control was 1.67 cm and 2.85 cm, respectively.

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“…In recent years, VOC's emitted by different strains of T. asperellum, such as: T1 (Wonglom et al 2020), LF15 (Sim et al 2019), LU1370 (Nieto-Jacobo et al 2017 and IsmT5 (Kottb et al 2015), have been analyzed. These investigations revealed the emission of 4 (Kottb et al 2015) to maximum 22 compounds (Kottb et al 2015;Wonglom et al 2019), whereas we detected 49 volatiles emitted by T. asperellum IZR D-11. Our results confirmed results from previous studies showing the presence of aliphatic alcohols e.g.…”

Section: Vocs Produced By Trichodermamentioning

confidence: 67%

“…Li et al (2018) found that T. asperellum, in contrast to T. virens or T. harzianum, did not secrete greater amount of volatiles in response to F. oxysporum infection. Moreover, Wonglom et al 2019 demonstrated that VOC's of T. asperellum can mediate antifungal activity, defense response and promote growth of lettuce (Lactuca sativa).…”

Section: Vocs Produced By Trichodermamentioning

confidence: 99%

Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides

Oszako

Войтка²,

Stocki

et al. 2020

Eur J Plant Pathol

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The protective effects of Trichoderma asperellum IZR D-11 as a biocontrol agent against the powdery mildew Erysiphe alphitoides infecting leaves of Quercus robur were evaluated for the first time. A strain of Trichoderma had earlier been isolated in Belarus, and was identified in this study as T. asperellum by sequencing of three genomic markers: internal transcribed spacer, translation elongation factor 1 alpha and RNA polymerase II subunit 2, with over 99.2% identity to corresponding genomic sequences in GenBank. When applied once in the first year just after onset of powdery mildew disease, T asperellum IZR D-11 reduced disease progression and continued to reduce powdery mildew levels during the following three years. Photosynthetic activity as represented by chlorophyll fluorescence measured in oak seedlings was increased in treated plants, and greater assimilate production was also found. The use of this antagonistic fungus increased the total water content in oak leaves suggesting that T. asperellum IZR D-11 can serve as a preventive measure to reduce energy losses in the process of water transpiration. GC-MS analysis detected 49 volatile compounds in the headspace of pure cultures of T. asperellum. Sesquiterpenes represented mainly by daucene, dauca-4(11),8-diene and isodaucene were the largest group of compounds emitted. We speculate that these volatiles from T. asperellum IZR D-11 may be involved in induced resistance in the plant, but further research is needed. The above results suggest that T. asperellum strain IZR D-11 has potential as a biocontrol agent of oak powdery mildew in forest nurseries.

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Biological control of Sclerotium fruit rot of snake fruit and stem rot of lettuce by Trichoderma sp. T76-12/2 and the mechanisms involved

Cited by 67 publications

References 21 publications

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Characterisation and antifungal activity of extracellular chitinase from a biocontrol fungus, Trichoderma asperellum PQ34

Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides

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