Biological control of take-all in wheat by endophytic Bacillus subtilis E1R-j and potential mode of action

Liu, Bing; Qiao, Hui; Huang, Lili; Buchenauer, H.; Han, Qingmei; Kang, Zhensheng; Gong, Yufei

doi:10.1016/j.biocontrol.2009.02.007

Cited by 121 publications

(73 citation statements)

References 26 publications

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“…Significant disease suppression was also reported for wheat plants endophytically colonised with Bacillus subtilis (Liu et al 2009). Another report stated that banana plants pre-inoculated with endophytic Pseudomonas and Burkholderia were able to suppress fungal disease caused by Fusarium oxysporum (Fishal et al 2010).…”

Section: Discussionmentioning

confidence: 88%

Effect of formulated bioorganic containing Burkholderia GanoEB2 in suppressing Ganoderma disease in oil palm seedlings

et al. 2015

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Section: Discussionmentioning

confidence: 88%

Effect of formulated bioorganic containing Burkholderia GanoEB2 in suppressing Ganoderma disease in oil palm seedlings

et al. 2015

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“…Novel secondary metabolites like lipopeptides, Oxylipins, Limnazine, beta-1,3-1, 4-glucanase are reported from bacillus Sps. (Gond et al, 2015, Trapp et al, 2015, Chen et al, 2013, Asolkar et al, 2002, Zouari et al, 2015, Jin et al, 2011, Liu et al, 2009. Enterobacter cloacae.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo antimicrobial activity of Ulva lactuca Linn. (Green algae) associated endophytic bacterial Strains

Habbu¹,

Warad²,

Shastri³

et al. 2016

J App Pharm Sci

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The development of resistance by pathogenic microorganisms to synthetic antibiotics encouraged researchers to find novel drugs for the treatment of infectious diseases. This opened new avenues to investigate the antimicrobial efficacy of active extracts/constituents from plant endophytes. In the present study, we have isolated endophytic bacteria (ULB-I, II and III) from green algae Ulva lactuca Linn. and the same bacteria were used for fermentation and extraction. Chloroform and ethyl acetate fractions of ULB-I, II and III were prepared and screened for in-vitro antimicrobial activities and also against Kleibesella pnumoniae infected mice in-vivo. In-vitro anti-microbial activities of ULB-I, II and III were performed against pathogenic bacteria, fungi and Mycobacterium tuberculosis H37 RV strain. From the phylogenetic analysis, the isolated endophytic organisms were identified as Bacillus subtilis JCM strain (ULB-I) and Enterobacter cloacae NBRC strain (ULB-II and III). ULB-I was found to be active against Staphylococcus aureus (1.6µg/ml) and Enterococcus feacalis (0.2µg/ml). The MIC against Staphylococcus aureus and Kleibesella pnumoniae was found to be 0.4µg/ml for ULB-II. A significant anti-fungal activity was observed against Aspergillus flavus (0.2-3.2µg/ml) and Aspergillus niger (0.2-0.4µg/ml). Further, Chloroform fraction of ULB-II and ethyl acetate fraction of ULB-III have shown significant anti-tubercular activity against the tested organism with MIC of 6.25µg/ml. This was supported by in-vivo antimicrobial activity against K. pneumoniae infection in mice and least hemolytic activity against erythrocytes was observed. HPTLC analysis of above fractions further confirmed the presence of polyvalent secondary metabolites.

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“…Recently, the use of biological control agents has received great attention because of the ability of such strains to suppress different plant diseases involving various modes of action (Cazorla et al 2006;Liu et al 2009;Sharma et al 2009) and the possibilities to be integrated with other control methods (Nofal and Haggag 2006;Pertot et al 2008;Correa et al 2009). Among the most promising candidates for bacterial biocontrol agents are several species of the genus Bacillus.…”

Section: Introductionmentioning

confidence: 99%

Iturin A is the principal inhibitor in the biocontrol activity ofBacillus amyloliquefaciensPPCB004 against postharvest fungal pathogens

Arrebola

Jacobs

Korsten

2010

Journal of Applied Microbiology

308

181

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Aims: A Bacillus amyloliquefaciens strain, surviving epiphytically on the surface of fruit, was isolated while searching for naturally occurring biological control agents. This bacterial strain was characterized for its antifungal activity against seven selected fungal postharvest pathogens of citrus. Methods and Results: To understand the antifungal activity, seven postharvest fungal pathogens were screened for growth inhibition by B. amyloliquefaciens strain. Assays using B. amyloliquefaciens lipopeptide extracts showed a strong inhibitive activity. The inhibitory effect was observed in abnormal conidial germination and germ tube development when conidia were treated with different lipopeptide extract concentrations. Further analysis using PCR and chromatography confirmed the presence of fengycin, iturin and surfactine, of which iturin A showed the strongest and most common inhibitory effect. The results are supported by site‐directed mutagenesis analysis, targeted to suppress the biosynthesis of iturin A production. Fruit trials confirmed disease development inhibition when the antagonist was applied 1 day prior to or 1 day after fungal application. Conclusions: We conclude that the iturin family of lipopeptides are vital in the antagonism of B. amyloliquefaciens against the seven citrus postharvest pathogenic fungi tested. Significance and Impact of the Study: We elucidated the principal mechanism used by B. amyloliquefaciens PPCB004 to suppress postharvest disease development on stored fruits.

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Biological control of take-all in wheat by endophytic Bacillus subtilis E1R-j and potential mode of action

Cited by 121 publications

References 26 publications

Effect of formulated bioorganic containing Burkholderia GanoEB2 in suppressing Ganoderma disease in oil palm seedlings

Effect of formulated bioorganic containing Burkholderia GanoEB2 in suppressing Ganoderma disease in oil palm seedlings

In vitro and in vivo antimicrobial activity of Ulva lactuca Linn. (Green algae) associated endophytic bacterial Strains

Iturin A is the principal inhibitor in the biocontrol activity ofBacillus amyloliquefaciensPPCB004 against postharvest fungal pathogens

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