Biological control of the potato dry rot caused by Fusarium species using PGPR strains

Kotan, Recep; Şahın, Fikrettin; Demirci, E.; Eken, Cafer

doi:10.1016/j.biocontrol.2009.04.004

Cited by 111 publications

(35 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chitinase (poly-beta-1, 4-[2-acetamido-2-deoxy]-δ-glucoside glycanohydrolase, EC 3.2.1.14), which is the first important enzyme of the two types of enzymes, plays a role in hydrolysis of chitin [17]. …”

Section: Resultsmentioning

confidence: 99%

Purification of Chitinase enzymes from Bacillus subtilis bacteria TV-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum

Senol

Nadaroğlu

Dikbaş

et al. 2014

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

BackgroundChitin is the main structural component of cell walls of fungi, exoskeletons of insects and other arthropods and shells of crustaceans. Chitinase enzyme is capable of degrading chitin, and this enzyme can be used as a biological fungicide against phytopathogenic fungi, as well as an insecticide against insect pests.MethodsIn this study, 158 isolates, which were derived from bacteria cultures isolated from leaves and root rhizospheres of certain plants in Turkey, were selected after confirming that they are not phytopathogenic based on the hypersensitivity test performed on tobacco; and antifungal activity test was performed against Fusarium culmorum, which is a pathogenic fungi that cause decomposition of roots of vegetables. Accordingly, chitinase enzyme activity assay was performed on 31 isolates that have an antifungal activity, and among them the isolate of Bacillus subtilis TV-125 was selected, which has demonstrated the highest activity.ResultsChitinase enzyme was purified by using ammonium sulphate and DEAE-sephadex ion exchange chromatography. Ammonium sulphate precipitation of chitinase enzyme from Bacillus subtilis TV-125 isolate was performed at maximum range of 0-20%, and 28.4-fold purification was obtained with a 13.4% of yield. Optimum activity of the purified enzyme was observed at pH 4.0 and at 50°C of temperature. In addition, it was identified that Bacillus subtilis TV-125A isolate retains 42% of its activity at 80°C temperature.ConclusionIn the last phase of the study, chitinase enzyme purified from Bacillus subtilis TV-125A was tested on four fungal agents, although all the results were positive, it was particularly effective on F. culmorum according to the findings.

show abstract

Section: Resultsmentioning

confidence: 99%

Purification of Chitinase enzymes from Bacillus subtilis bacteria TV-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum

Senol

Nadaroğlu

Dikbaş

et al. 2014

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

show abstract

“…Within microbial-based biopesticides, the use of antagonistic microorganisms, mostly bacteria and fungi, has shown their efficiency to control different postharvest rot pathogens of sweet cherries and table grapes [105], banana [27], citrus [18], pineapple [124], apple [76,110], peach [95], potato [99], tomato [70], and mandarin [59], among others. However, this type of biopesticides presents some disadvantages according to tests conducted under commercial or semicommercial conditions.…”

Section: Parameters Involved In Fruit and Vegetables Postharvest Lossesmentioning

confidence: 99%

Perspectives on Utilization of Edible Coatings and Nano-laminate Coatings for Extension of Postharvest Storage of Fruits and Vegetables

Flores-López¹,

Cerqueira²,

Rodríguez

2015

Food Eng Rev

160

View full text Add to dashboard Cite

It is known that in developing countries, a large quantity of fruit and vegetable losses results at postharvest and processing stages due to poor or scarce storage technology and mishandling during harvest. The use of new and innovative technologies for reducing postharvest losses is a requirement that has not been fully covered. The use of edible coatings (mainly based on biopolymers) as a postharvest technique for agricultural commodities has offered biodegradable alternatives in order to solve problems (e.g., microbiological growth) during produce storage. However, biopolymer-based coatings can present some disadvantages such as: poor mechanical properties (e.g., lipids) or poor water vapor barrier properties (e.g., polysaccharides), thus requiring the development of new alternatives to solve these drawbacks. Recently, nanotechnology has emerged as a promising tool in the food processing industry, providing new insights about postharvest technologies on produce storage. Nanotechnological approaches can contribute through the design of functional packing materials with lower amounts of bioactive ingredients, better gas and mechanical properties and with reduced impact on the sensorial qualities of the fruits and vegetables. This work reviews some of the main factors involved in postharvest losses and new technologies for extension of postharvest storage of fruits and vegetables, focused on perspective uses of edible coatings and nano-laminate coatings.

show abstract

“…In a later study, the use of this approach to compare tillage and NT in Cerrado soils was shown to be efficient in detecting changes in the soil bacterial community structure (BCS) caused by alterations in soil conditions (Peixoto et al 2005). The authors suggested that it would also be important to evaluate alterations in the profiles of some specific microbial groups of agricultural interest, such as the Pseudomonas group, which has a prominent role in several processes important to soil quality and functioning and includes many species of plant growth-promoting rhizobacteria (PGPR) (Timms-Wilson et al 2004;Bardas et al 2009;Kumar et al 2009;Recep et al 2009;Rosas et al 2009). …”

Section: Introductionmentioning

confidence: 97%

A decade of land use contributes to changes in the chemistry, biochemistry and bacterial community structures of soils in the Cerrado

Peixoto

Chaer

Franco

et al. 2010

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

The bacterial community structures (BCSs) of Cerrado soils cultivated under conventional tillage (CT), no-tillage (NT) and under native Cerrado (NC) vegetation were evaluated using PCR/DGGE of bacterial 16S rRNA (rrs) and rpoB genes and of Pseudomonas group genes. Soil chemical analysis, microbial biomass and the enzyme activities were also evaluated and correlated with the BCS measurements. The multivariate ordinations of DGGE profiles showed differences between the BCS of the NC area and those from cultivated areas. The BCSs of the CT and NT areas also differed in all DGGE fingerprints, including changes in the profile of Pseudomonas populations, indicating that agricultural systems can also be responsible for changes within specific microbial niches, although the clearest differences were found in the rpoB profiles. The MRPP analysis demonstrated significant differences between the BCSs from different soil layers of NT areas based on all gene fingerprints and those of NC areas based on bacterial 16S rRNA and rpoB genes fingerprints. No differences were observed in the microbial fingerprints of CT samples from different depths, indicating that ploughing affected the original BCS stratification. The BCS from NC areas, based on all gene fingerprints, could be related to higher levels of soil acidity and higher amounts of MBC and of phosphatase activity. In contrast, the BCSs from cultivated areas were related to higher levels of Ca + Mg, P and K, likely as a result of a history of chemical fertilisation in these areas. The relationships between rpoB and Pseudomonas BCSs and all chemical and biochemical properties of soils were significant, according to a Mantel test (P < 0.05), indicating that the different changes in soil properties induced by soil use or management may drive the formation of the soil BCS.

show abstract

Biological control of the potato dry rot caused by Fusarium species using PGPR strains

Cited by 111 publications

References 24 publications

Purification of Chitinase enzymes from Bacillus subtilis bacteria TV-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum

Purification of Chitinase enzymes from Bacillus subtilis bacteria TV-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum

Perspectives on Utilization of Edible Coatings and Nano-laminate Coatings for Extension of Postharvest Storage of Fruits and Vegetables

A decade of land use contributes to changes in the chemistry, biochemistry and bacterial community structures of soils in the Cerrado

Contact Info

Product

Resources

About