Biological decolorization of Amaranth dye with Trametes polyzona in an airlift reactor under three airflow regimes

Abstract

In the present work, a strain of the basidiomycete fungus Trametes polyzona was used to decolorize the Amaranth dye. The decolorization was carried out in an Airlift reactor with three flow regimes: 1, 2, and 3 vvm. The results showed that the decolorization was a function of the flow regime. The decolorization times for the regimes of 1, 2, and 3 vvm were 30, 25, and 19 days, respectively. The COD (Chemical Oxygen Demand) decreased from 1600 to 72 mg COD/L. The enzymatic activity kinetics of laccase (Lcc), lignin peroxidase (LiP), and manganese peroxidase (MnP) were determined. In all the treatments, the enzyme LiP was expressed during the first 6 days, at which point 80% decolorization was observed, whereas Lcc and MnP enzymes were produced from day 6 until the end of the decolorization process. The effluent generated showed no inhibitory effects on the growth of the algae Nannochloropsis salina. T. polyzona showed great versatility in the decolorization of synthetic effluents containing the Amaranth dye, and the fungus was able to use this dye as its only carbon source starting at the beginning of the process. LiP was the enzyme that contributed the most to the decolorization process, and on average, 95% decreases in color and the COD were observed.

Morphological, Molecular Characterization, and Physico-chemical Analysis of Trichoderma yunnanense as Indigosol Golden Yellow Dye-decolorizing Fungus

“…After being treated with the addition of T. yunnanense isolate, the pH value was decreased in the range of 7.14. In this study, high levels of BOD (21,881.34 mg L -1 ) and COD (50,909.09 mg L -1 ) in batik waste indicated high toxicity, so it is very dangerous to environment and health human (Pérez-Cadena et al 2020). The decrease in BOD and COD levels by T. yunnanense can result from the removal of organic loads from batik waste that reduce toxicity.…”

Decolorization of reactive blue 13 by Sporotrichum sp. and cytotoxicity of biotreated dye solution

“…Among them, agitation can affect fungi by supplying oxygen and nutrients, and also, it can affect the mycelial network by damaging the fungi. Perez-Cadena et al (2020) observed that decolorization efficiency of azo dyes by Trametes polyzona increased by increased agitation rate. They argued that with the increase of aeration, the biomass increases, and this positively affects the electron transfer that results in increased enzymatic activity (Perez-Cadena et al, 2020;Hadibarata and Kristanti., 2012).…”

“…Perez-Cadena et al (2020) observed that decolorization efficiency of azo dyes by Trametes polyzona increased by increased agitation rate. They argued that with the increase of aeration, the biomass increases, and this positively affects the electron transfer that results in increased enzymatic activity (Perez-Cadena et al, 2020;Hadibarata and Kristanti., 2012). In our case, because there is no nutrient in the solution, it can be anticipated that the increase in the amount of oxygen with agitation and the fragmentation of the mycelium played a positive role on the adsorption rate.…”

Application of myco-light expanded clay aggregate for real textile wastewater treatment in rotating drum biological contactor

“…Pakshirajan & Kheria (2012) observed an agreement of 64% decolorization efficiency with 73% COD reduction of diluted textile wastewater by immobilized P. chrysosporium within 2 days [23]. Moreover, other research showed similar relation with longer treatment days between 12−70 days in the optimum treatment conditions and/or by using synthetic dyes [37,38]. A recent study by Dalecka et al…”