Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex

Komaba, Shinichi; Fujino, Yusuke; Matsuda, Tomoka; Osaka, Tetsuya; Satoh, Isao

doi:10.1016/s0925-4005(98)00259-7

Cited by 30 publications

(18 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Meanwhile, the mixture (550 nM for Cys, 88 μM for His, Ser, Ala, and Gly) also made the fluorescence significantly decrease, suggesting that the coexistence of other amino acids in the solution did not affect the Cys detection. Although some amino acids, such as Lys and Met have been found to combine with Ag + as well [30], the interaction of them was not strong enough to destroy the C-Ag + -C base pairs here. Therefore, this method has high selectivity, which can be applied in amino acids mixed samples.…”

Section: Sensitivity and Selectivity For Ag + Detectionmentioning

confidence: 78%

Fluorescent detection of silver(I) and cysteine using SYBR Green I and a silver(I)-specific oligonucleotide

Zhao

Huang

et al. 2012

Microchim Acta

View full text Add to dashboard Cite

We report on a novel method for the determination of silver ion (Ag + ) and cysteine (Cys) by using the probe SYBR Green I (SGI) and an Ag+-specific cytosinerich oligonucleotide (C-DNA). The fluorescence of SGI is very weak in the absence or presence of randomly coiled C-DNA. If, however, C-DNA interacts with Ag + through the formation of cytosine-Ag + -cytosine (C-Ag + -C) base pairs, the randomly coiled C-DNA undergoes a structural changes to form a hairpin-like structure, thereby increasing the fluorescence of SGI. This fluorescence turn-on process allows the detection of Ag + in the 10-600 nM concentration range, with a detection limit of 4.3 nM. Upon the reaction of Ag + with Cys, Cys specifically removes Ag + from the C-Ag + -C base pairs and destroys the hairpin-like structure. This, in turn, results in a decrease in fluorescence intensity. This fluorescence turn-off process enables the determination of Cys in the 8-550 nM concentration range, with a detection limit of 4.5 nM. The method reported here for the determination of either Ag + or Cys is simple, sensitive, and affordable, and may be applied to other detection systems if appropriately selected DNA sequences are available.

show abstract

Section: Sensitivity and Selectivity For Ag + Detectionmentioning

confidence: 78%

Fluorescent detection of silver(I) and cysteine using SYBR Green I and a silver(I)-specific oligonucleotide

Zhao

Huang

et al. 2012

Microchim Acta

View full text Add to dashboard Cite

show abstract

“…After preparing PIC membranes containing enzymes, polypyrrole can be synthesized in the membranes electrochemically. [48][49][50] Even though other polymers can be synthesized in the PIC, the PIC membranes are not destroyed, but can be used for biosensors.…”

Section: ·1mentioning

confidence: 99%

Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

Yabuki

2011

ANAL. SCI.

View full text Add to dashboard Cite

The immobilization of biomolecules is an important technique for bio-analysis, and can be applied to biosensors with both high selectivity and high sensitivity. Many researchers have developed immobilization techniques to optimize these characteristics. In the last two decades, an immobilization technique that meets the desired requirements was developed by using polyelectrolytes to form complexes, based on the electrostatic binding between polycations and polyanions. This review summarizes the techniques used for the immobilization of biomolecules by polyelectrolyte complexes; it also discusses related subjects.

show abstract

“…However, in comparison with the previously reported biosensors for L-arginine [16][17][18][19][20][21], the detection limit and range of the linearity of the developed here biosensors (the biosensor control and the biosensor Variant 1) are very promising and comparable with that obtained for the potentiometric biosensor developed by Koncki et al [19]. At the same time, all the clinoptilolite-based biosensors had significantly shorter response time than that of the biosensor reported in [19]: (46±0.6)-(58±0.6) s versus 90-240 s, respectively.…”

Section: Electrical Properties and Analytical Characteristics Of Condmentioning

confidence: 71%

“…To date, a great number of publications have reported about L-arginine biosensors based on amperometric [13][14][15] and potentiometric [16][17][18][19][20][21] transduction modes. For example, in [15] a biosensor system, consisting of amperometric biosensors for determination of L-arginine, L-histidine, Llysine and L-glutamic acid, is presented.…”

Section: Introductionmentioning

confidence: 99%

Application of Ammonium-Selective Zeolite for Enhancement of Conductometric Bi-Enzyme Biosensor for L-Arginine Detection

Saiapina

Matsishin

Pyeshkova

et al. 2012

SEMST

View full text Add to dashboard Cite

Abstract. L-arginine conductometric biosensors were developed on the basis of arginase and urease cross-linked by glutaraldehyde in a single bioselective membrane and modified with clinoptilolite. The clinoptilolite intrinsic properties helpful for conductometric detection of L-arginine were investigated using three approaches to the creation of zeolite-containing enzymatic membranes. The developed biosensors were compared with the L-arginine biosensor, not modified with clinoptilolite, for the sensitivity, linear and dynamic range, detection limit, response time, operational and storage stability in L-arginine analysis. It was shown that the incorporation of ammonium-selective zeolite to the bioselective membrane of L-arginine biosensor allows increasing the biosensor sensitivity when applying all proposed approaches to the formation of zeolite-containing biomembranes. In addition, excellent values of both detection limit and linear range (1.0×10-5 M and 0.01-6 mM, respectively) were achieved for the biosensor based on arginase, urease and zeolite distributed in a single bioselective layer. The clinoptilolite-based biosensors for L-arginine demonstrated short response time, high operational stability (a coefficient of variations reached 0.74%), their lifetime exceeded four months.Keywords: L-arginine; Clinoptilolite; Conductometric biosensor; Arginase; Urease ЗАСТОСУВАННЯ АМОНІЙ-СЕЛЕКТИВНОГО ЦЕОЛІТУ ДЛЯ ПОКРАЩЕННЯ КОНДУКТОМЕТРИЧНОГО ДВОФЕРМЕНТНОГО БІОСЕНСОРА ДЛЯ ВИЗНАЧЕННЯАнотація. Розроблено кондуктометричні біосенсори для визначення L-аргініну на основі аргінази та уреази, іммобілізованих в одній біоселективній мембрані методом поперечного зшивання за допомогою глутарового альдегіду та модифікованій клиноптилолітом. Характерні властивості клиноптилоліту, корисні для кондуктометричного визначення L-аргініну, були до-слідженні за допомогою трьох підходів для створення цеоліт-вмісних ферментних мембран. Розроблені біосенсори порівнювали з біосенсором для визначення L-аргініну, який не був мо-дифікований клиноптилолітом, за такими показниками, як чутливість, лінійний і динамічний діапазон, нижня межа визначення, час відгуку, операційна стабільність і стабільність при збе-ріганні. Показано, що включення амоній-селективного цеоліту до складу біоселективної мемб-рани L-аргінінового біосенсора дозволяє підвищити чутливість біосенсора, застосовуючи всі запропоновані підходи для формування цеоліт-вмісних біомембран. Крім того було отримано чудові значення нижньої межі визначення та лінійного діапазону (1,0×10-5 М та 0,01-6 мМ від-повідно) у біосенсора, у якому аргіназа, уреаза та цеоліт були розподілені в єдиному біоселек-тивному шарі. Біосенсори для визначення L-аргініну на основі клиноптилоліту мали короткий час відгуку, високу операційну стабільність (коефіцієнт варіації досягав 0,74%), а їх стабіль-ність при зберіганні була вища за чотири місяці. Аннотация. Разработаны кондуктометрические биосенсоры для определения L-аргинина на основе аргиназы и уреазы, иммобилизованных в одной биоселективной мембране методом попе...

show abstract

Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex

Cited by 30 publications

References 11 publications

Fluorescent detection of silver(I) and cysteine using SYBR Green I and a silver(I)-specific oligonucleotide

Fluorescent detection of silver(I) and cysteine using SYBR Green I and a silver(I)-specific oligonucleotide

Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

Application of Ammonium-Selective Zeolite for Enhancement of Conductometric Bi-Enzyme Biosensor for L-Arginine Detection

Contact Info

Product

Resources

About