Biological effects and clinical characteristics of microRNA-106a in human colorectal cancer

Abstract

MicroRNAs serve important roles in various diseases, particularly cancer. microRNA-106a (miR-106a) exhibits abnormal expression and oncogenic activity in carcinogenesis. The clinical significance of the abnormal expression of miR-106a in colorectal cancer is poorly understood. In the present study, miR-106a expression from colorectal cancer tissues was quantified using the reverse transcription-quantitative polymerase chain reaction. The overexpression or knockdown of miR-106a was performed by transfection with microRNA mimic or inhibitor in human colorectal carcinoma HCT116 cells. The overexpression of miR-106a promoted viability and inhibited apoptosis in colorectal cancer cells. The association between miR-106a expression and clinicopathological factors was analyzed, and it was identified that miR-106a exhibited significantly increased expression in adenocarcinoma tissues compared with in mucinous carcinoma tissues, and the expression of miR-106a was identified to be associated with the depth of invasion and differentiation. The expression of miR-106a in plasma was also determined and it was identified that increased expression of miR-106a, as a characteristic of patients with colorectal cancer, may be distinguished from that of other patients by digitization of the areas under the receiver operating characteristic curves. These data suggested that miR-106a is a potential biomarker in the diagnosis of colorectal carcinoma. However, the underlying molecular mechanism of miR-106a-promoted viability and inhibition of apoptosis requires further investigation.

Biomarker roles identification of miR-106 family for predicting the risk and poor survival of colorectal cancer

“…1, on the basis of initial literature research, a total of 225 qualified articles were involved from the selected databases. According to the inclusion and exclusion criteria, after removing the duplicates and reviewing the texts, 19 articles including 28 studies were utilized for the final analysis, of which 6 studies were about the value of miR-106 family for CRC diagnosis and 22 studies were about CRC prognosis [8,[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. All studies applied quantitative reverse transcription PCR (qRT-PCR) to measure the expression of miR-106…”

Clinical significance of tumor miR-21, miR-221, miR-143, and miR-106a as biomarkers in patients with osteosarcoma

“…It is down-regulated in many human malignant tumors. 9 miR-106a is composed of 22 nucleotides, and is located in the q26.2 region of the X chromosome. The miR-106a expression is closely related with tumor cell division, proliferation, apoptosis, and drug resistance.…”

Biomarker roles identification of miR-106 family for predicting the risk and poor survival of colorectal cancer

“…As shown in Figure 1, on the basis of initial literature research, a total of 225 qualified articles were involved from the selected databases. According to the inclusion and exclusion criteria, after removing the duplicates and reviewing the texts, 19 articles including 28 studies were utilized for the final analysis, of which 6 studies were about the value of miR-106 family for CRC diagnosis and 22 studies were about CRC prognosis [8,[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. All studies applied quantitative reverse transcription PCR (qRT-PCR) to measure the expression of miR-106 family.…”