Biological effects of low-level laser irradiation on umbilical cord mesenchymal stem cells

Abstract

Low-level laser irradiation (LLLI) can enhance stem cell (SC) activity by increasing migration and proliferation. This study investigated the effects of LLLI on proliferation, enzymatic activity, and growth factor production in human umbilical cord mesenchymal SCs (hUC-MSCs) as well as the underlying mechanisms. hUC-MSCs were assigned to a control group (non-irradiation group) and three LLLI treatment groups (635 nm group, 808 nm group, and 635/808 nm group). Laser power density and energy density of 20 mW/cm2 and 12 J/cm2, respectively, were used for each experiment. The proliferation rate was higher in the 635 nm as compared to the other groups. LLLI at 808 nm did not induce cell proliferation. ROS levels in cells exposed to 635, 808, and 635/808 nm radiation were increased by 52.81%, 26.89%, and 21.15%, respectively, relative to the control group. CAT, tGPx, and SOD activity was increased. LLLI at 808 nm increased the levels of IL-1, IL-6, and NFκB but not VEGF. LLLI improved hUC-MSCs function and increased antioxidant activity. Dual-wavelength LLLI had more potent effects on hUC-MSCs than single-wavelength treatment. LLLI has potential applications in the preconditioning of hUC-MSCs in vitro prior to transplantation, which could improve the regenerative capacity of cells.

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

“…LLLT at 4 J/cm 2 partially reversed the activation of DNA oxidation, caspase 3/8, IL-1β/6 and IFN-γ induced by H 2 O 2 , with an increased level of anti-inflammatory IL-10. Similar to the findings by Fernandes on M1 macrophage, a report by Chen et al on human umbilical cord mesenchymal stem cells found that LLLT (635 nm, 808 nm, 635 + 808 nm, 12 J/cm 2 , two daily treatments for 3 days) promoted the expressions of proinflammatory cytokines IL-1, IL-6 along with NF-κB (41), but this was also accompanied by an increase in cell proliferation.…”

The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review

“…25 A laser with a wavelength of 635nm amplified the umbilical cord mesenchymal cells if it proved that a low-power laser with 808nm failed to increase the proliferation of the same cells. 26 Adipose Mesenchymal Stem Cells It has been reported that the toxic effects of doxorubicin on the adipose mesenchymal stem cells can be reduced by using a low-power laser with a wavelength of 660 nm, output power of 30 mW, a laser beam of 0.028 cm 2 , and irradiation of 1.07 mW/cm 2 . A Low-power laser with these features increases the viability of the MSCs and inhibits apoptosis and oxidative stress in the MSCs that are treated with doxorubicin.…”

Effect of Low-Level Laser Irradiation on the Function of Glycated Catalase

“…12 Later in another work, they used 635 nm and 808 nm laser irradiation on stem cells. 21 They used these wavelengths separately and also as dual-wavelength laser and they concluded using both wavelengths on one sample could be more effective. 21 In another study, Unnikrishna Pillai and Padma used laser irradiations with wavelengths 476 nm, 488 nm, and 514 nm on seeds, and concluded that, the 476 nm wavelength had more effect on increasing the activity of catalase.…”

“…21 They used these wavelengths separately and also as dual-wavelength laser and they concluded using both wavelengths on one sample could be more effective. 21 In another study, Unnikrishna Pillai and Padma used laser irradiations with wavelengths 476 nm, 488 nm, and 514 nm on seeds, and concluded that, the 476 nm wavelength had more effect on increasing the activity of catalase. 22 Many different studies were conducted to investigate the effect of laser irradiation on parotid glands in diabetes rats.…”