Biological effects of poly(ethylene glycol) on the microbial poly(β-hydroxyalkanoates) produced by pseudomonads microorganisms

PEGylation and BioPEGylation of Polyhydroxyalkanoates: Synthesis, Characterisation and Applications

“…The addition of comparatively low molecular weight PEGs to bioprocessing systems using P. oleovorans and P. putida induced manipulation of mcl-PHAs molecular weight. Additions of 2% (w/v) of PEG-106, PEG-200 or PEG-400 reduced the molecular weights of PHO synthesised by these species (Kim, 2000;Sanguanchaipaiwong et al 2004). However, the impact appeared to be greater for P. oleovorans that P. putida with this mcl-PHA reduced to 40 and 80% respectively (Kim 2000).…”

“…This increase in cell mass is consistent with the studies of Shah et al who reported that EG was metabolised by R. eutropha for the production of PHB (Shah et al 2000a) Microbial PHA synthesis is species dependent; the diversity of fcl-and mcl-PHAs are significantly greater than their sclPHA companions. Kim investigated the influence of PEG-200 on the synthesis of mcl-PHAs by P. oleovorans and P. putida and reported that PHO yield was reduced with increasing PEG concentration, while at 8% PEG-200 loading no PHO synthesis was found (Kim, 2000). Using the same strain of P. oleovorans cultivated in the www.intechopen.com…”

Biosynthesis of Natural-Synthetic Hybrid Copolymers: Polyhydroxyoctanoate−Diethylene Glycol

“…These parameters are influenced by the molecular weight of the PEG, its concentration, and the PHB producing microbial species. [18][19][20][21][22][23] Furthermore, Gross and coworkers reported the potential for microbial species to incorporate PEG units at the synthetic terminus of the PHB chain, i.e., "PEGylation". 18 A family of PEGs includes members that are biocompatible and serve a number of important biomedical functions such as organ and tissue preservation.…”

Copolymers of polyhydroxyalkanoates and polyethylene glycols: recent advancements with biological and medical significance

“…The influence of PEGs on PHA bioprocessing parameters was also investigated and shown to be species dependent . For some species, polyhydroxyoctanoate (PHO) yield was reduced with increasing PEG concentration, while with 8% of PEG loading no PHO synthesis was found …”

“…27 For some species, polyhydroxyoctanoate (PHO) yield was reduced with increasing PEG concentration, while with 8% of PEG loading no PHO synthesis was found. 24,28 In terms of synthetic PEGylation of PHAs, examples are 'classical' block copolymers formed via ester formation, 29 via polyurethane formation, 30 (amphiphilic) diblock copolymers prepared via azide click chemistry 31 and PHA-based graft polymers prepared via thiol-ene chemistry (see later). 32 With regard to the impact of PHA-PEG copolymers, both components are US Food and Drug Administration approved and recent progress in their development suggests that such conjugates have significant potential for medical applications.…”