Biological functions of Arabidopsis thaliana MBP‐1‐like protein encoded by ENO2 in the response to drought and salt stresses

Abstract

Arabidopsis thaliana ENO2 (AtENO2) plays an important role in plant growth and development. It encodes two proteins, a full‐length AtENO2 and a truncated version, AtMBP‐1, alternatively translated from the second start codon of the mRNA. The AtENO2 mutant (eno2−) exhibited reduced leaf size, shortened siliques, a dwarf phenotype and higher sensitivity to abiotic stress. The objectives of this study were to analyze the regulatory network of the ENO2 gene in plant growth development and understand the function of AtENO2/AtMBP‐1 to abiotic stresses. An eno2−/35S:AtENO2‐GFP line and an eno2−/35S:AtMBP‐1‐GFP line of Arabidopsis were obtained. Results of sequencing by 454 GS FLX identified 578 upregulated and 720 downregulated differential expressed genes (DEGs) in a pairwise comparison (WT‐VS‐eno2−). All the high‐quality reads were annotated using the Gene Ontology (GO) terms. The DEGs with KEGG pathway annotations occurred in 110 pathways. The metabolic pathways and biosynthesis of secondary metabolites contained more DEGs. Moreover, the eno2−/35S:AtENO2‐GFP line returned to the wild‐type (WT) phenotype and was tolerant to drought and salt stresses. However, the eno2−/35S:AtMBP‐1‐GFP line was not able to recover the WT phenotype but it has a higher tolerance to drought and salt stresses. Results from this study demonstrate that AtENO2 is critical for the growth and development, and the AtMBP‐1 coded by AtENO2 is important in tolerance of Arabidopsis to abiotic stresses.

ENO2 Affects the Seed Size and Weight by Adjusting Cytokinin Content and Forming ENO2-bZIP75 Complex in Arabidopsis thaliana

“…Eremina et al (2015) pointed that ENO2 mutants and AtMBP-1 overexpressing plants show a strong phenotypic resemblance with other mutants impaired in glycolysis, and the constitutive developmental defects in plant are correlated with a strong reduction in enolase activity, but not with a reduction in AtMBP-1 transcript abundance. Our previous study also demonstrated that AtMBP-1 is predominant in tolerance of adult Arabidopsis to abiotic stresses (Liu Z. J. et al, 2019).…”

“…The ENO gene are widely distributed and highly conserved in vertebrates and plants which encodes a glycolytic metalloenzyme enolase (ENO2) that catalyzes the dehydration of 2-phospho-Dglycerate (2-PGA) to phosphoenolpyruvate (PEP) (Liu Z. J. et al, 2019). In Arabidopsis, there are totally three ENO genes, At1g74030 (ENO1), At2g36530 (ENO2, also referred to LOS2, Lee et al, 2002) and At2g29560 (ENO3).…”

Comparative Analysis on the Evolution of Flowering Genes in Sugar Pathway in Brassicaceae

“…The Arabidopsis pgm2 pgm3 double mutant has been reported to markedly damage the function of male and female gametophytes [ 57 ]. ENOLASE 2 (ENO2) maintains normal growth, development, and successful reproduction in plants and is also able to help plants respond to abiotic stresses such as drought and cold [ 58 , 59 , 60 ]. ENOLASE 1 (ENO1) plays a crucial role in the development of gametophytes and sporophytes [ 61 ].…”

Enolase2 regulates seed fatty acid accumulation via mediating carbon partitioning in Arabidopsis thaliana

“…SA delays fruit ripening by inhibiting ethylene biosynthesis (Kumar et al, 2021; Srivastava & Dwivedi, 2000). Either increased auxin level or decreased ethylene content delays fruit ripening (Barry & Giovannoni, 2007; Liu, Zhang, et al, 2020; Sravankumar et al, 2018). Our previous research also showed that the absence of ENO2 elevated the level of auxin and decreased the ethylene content in A. thaliana seeds (Liu, Zheng, et al, 2020).…”

“…Enolase (ENO, 2‐phospho‐ d ‐glycerate hydrolyase), a metalloenzyme in glycolysis, which catalyzes the dehydration of 2‐phospho‐ d ‐glycerate to phosphoenolpyruvate, are widely distributed and highly conserved in vertebrates and plants (Liu, Zhang, et al, 2020). There are three ENO genes in A. thaliana : AtENO1 (At1g74030), AtENO2 (At2g36530, also referred to LOS2 ) (Lee et al, 2002), and AtENO3 (At2g29560).…”