Biological insights into the piericidin family of microbial metabolites

Azad, Sepideh M; Jin, Yu; Ser, Hooi‐Leng; Goh, Bey Hing; Lee, Learn‐Han; Thawai, Chitti; He, Yanan

doi:10.1111/jam.15222

Cited by 11 publications

(6 citation statements)

References 53 publications

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“…Correspondingly, at the metabolome level, several metabolites were uniquely detected in the co‐cultures or the single cultures (Tables S3 and S4 ). In AtB‐42, for example, the co‐culturing induced the production of glucopiericidin and hampered that of cyclipostin, ripromycin, WK 142B, delaminomycin A and Formycin A, which are well‐known antibiotics of the Streptomyces genus (Azad et al, 2022 ; Bertasso et al, 2003 ; Hori et al, 1964 ; Omura et al, 1986 ; Ueno et al, 1993 ; Wink et al, 2002 ). It should be mentioned that, besides the huge literature on the co‐cultures of microorganisms, few articles report the enhancement of the overall production of metabolites.…”

Section: Discussionmentioning

confidence: 99%

Interactions between plant‐beneficial microorganisms in a consortium: Streptomyces microflavus and Trichoderma harzianum

Prigigallo

Staropoli

Vinale

et al. 2023

Microbial Biotechnology

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The construction of microbial consortia is challenging due to many variables to be controlled, including the cross‐compatibility of the selected strains and their additive or synergistic effects on plants. In this work, we investigated the interactions in vitro, in planta, and at the molecular level of two elite biological control agents (BCAs), that is Streptomyces microflavus strain AtB‐42 and Trichoderma harzianum strain M10, to understand their attitude to cooperate in a consortium. In vitro, we observed a strong cross‐antagonism between AtB‐42 and M10 in agar plates due to diffusible metabolites and volatile organic compounds. In liquid co‐cultures, M10 hindered the growth of AtB‐42 very likely because of secondary metabolites and strong competition for the nutrients. The interaction in the co‐culture induced extensive transcriptional reprogramming in both strains, especially in the pathways related to ribosomes, protein synthesis, and oxidoreductase activity, suggesting that each strain recognized the counterpart and activated its defence responses. The metabolome of both strains was also significantly affected. In contrast, in the soil, M10 growth was partially contrasted by AtB‐42. The roots of tomato seedlings inoculated with the consortium appeared smaller than the control and single‐strain‐inoculated plants, indicating that plants diverted some energy from the development to defence activation, as evidenced by the leaf transcriptome. The consortium induced a stronger transcriptional change compared to the single inoculants, as demonstrated by a higher number of differentially expressed genes. Although the cross‐antagonism observed in vitro, the two strains exerted a synergistic effect on tomato seedlings by inducing resistance responses stronger than the single inoculants. Our observations pose a question on the usefulness of the sole in vitro assays for selecting BCAs to construct a consortium. In vivo experiments should be preferred, and transcriptomics may greatly help to elucidate the activity of the BCAs beyond the phenotypic effects on the plant.

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Section: Discussionmentioning

confidence: 99%

Interactions between plant‐beneficial microorganisms in a consortium: Streptomyces microflavus and Trichoderma harzianum

Prigigallo

Staropoli

Vinale

et al. 2023

Microbial Biotechnology

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“…The piericidin family is an important kind of active microbial metabolite that features a 4-pyridinol core linked with a methylated polyketide side chain [23]. Accumulating evidence suggests that natural piericidins obtained from marine-derived Streptomyces have received considerable attention in recent years because of their important medicinal activities [23,24]. Our group has previously obtained 43 natural piericidins from two strains of marine-derived Streptomyces, including 29 new compounds [25][26][27], and found that the main piericidin metabolites, including piericidin A (PA) and glucopiericidin A (GPA), have the potential to be developed as potential antirenal carcinoma drugs.…”

Section: Introductionmentioning

confidence: 99%

Marine-Derived Piericidin Diglycoside S18 Alleviates Inflammatory Responses in the Aortic Valve via Interaction with Interleukin 37

She

Zeng

et al. 2022

Oxidative Medicine and Cellular Longevity

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Calcific aortic valve disease (CAVD) is a valvular disease frequently in the elderly individuals that can lead to the valve dysfunction. Osteoblastic differentiation of human aortic valve interstitial cells (HAVICs) induced by inflammation play a crucial role in CAVD pathophysiological processes. To date, no effective drugs for CAVD have been established, and new agents are urgently needed. Piericidin glycosides, obtained from a marine-derived Streptomyces strain, were revealed to have regulatory effects on mitochondria in previous studies. Here, we discovered that 13-hydroxypiericidin A 10-O-α-D-glucose (1→6)-β-D-glucoside (S18), a specific piericidin diglycoside, suppresses lipopolysaccharide- (LPS) induced inflammatory responses of HAVICs by alleviating mitochondrial stress in an interleukin (IL)-37-dependent manner. Knockdown of IL-37 by siRNA not only exaggerated LPS-induced HAVIC inflammation and mitochondrial stress but also abrogated the anti-inflammatory effect of S18 on HAVICs. Moreover, S18 alleviated aortic valve lesions in IL-37 transgenic mice of CAVD model. Microscale thermophoresis (MST) and docking analysis of five piericidin analogues suggested that diglycosides, but not monoglycosides, exert obvious IL-37-binding activity. These results indicate that S18 directly binds to IL-37 to alleviate inflammatory responses in HAVICs and aortic valve lesions in mice. Piericidin diglycoside S18 is a potential therapeutic agent to prevent the development of CAVD.

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“…Piericidins are a class of 4-pyridinol alkaloids decorated with methylated polyene side chains, mostly isolated from actinomycetes of soil, marine or bio-symbiotic origin [73]. Due to structural similarities with coenzyme Q, some piericidins exhibit NADH−ubiquinone oxidoreductase inhibitory activity [74]. Moreover, insecticidal, cytotoxic, and bactericidal effects of piericidins have been reported.…”

Section: Piericidinsmentioning

confidence: 99%

Marine Streptomyces-Derived Novel Alkaloids Discovered in the Past Decade

Liu,

Sun,

et al. 2024

Marine Drugs

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Natural alkaloids originating from actinomycetes and synthetic derivatives have always been among the important suppliers of small-molecule drugs. Among their biological sources, Streptomyces is the highest and most extensively researched genus. Marine-derived Streptomyces strains harbor unconventional metabolic pathways and have been demonstrated to be efficient producers of biologically active alkaloids; more than 60% of these compounds exhibit valuable activity such as antibacterial, antitumor, anti-inflammatory activities. This review comprehensively summarizes novel alkaloids produced by marine Streptomyces discovered in the past decade, focusing on their structural features, biological activity, and pharmacological mechanisms. Future perspectives on the discovery and development of novel alkaloids from marine Streptomyces are also provided.

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Biological insights into the piericidin family of microbial metabolites

Cited by 11 publications

References 53 publications

Interactions between plant‐beneficial microorganisms in a consortium: Streptomyces microflavus and Trichoderma harzianum

Interactions between plant‐beneficial microorganisms in a consortium: Streptomyces microflavus and Trichoderma harzianum

Marine-Derived Piericidin Diglycoside S18 Alleviates Inflammatory Responses in the Aortic Valve via Interaction with Interleukin 37

Marine Streptomyces-Derived Novel Alkaloids Discovered in the Past Decade

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