Biological near-field scanning optical microscopy: instrumentation and sample issues for shear-force feedback

Valaskovic, Gary A.; Holton, Mark; Morrison, George H.

doi:10.1016/0304-3991(94)00138-d

Cited by 12 publications

(6 citation statements)

References 11 publications

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“…Most schemes introduced for NSOM revolve around force measurements between an oscillating tip and the sample surface. A popular method is the shear-force approach where the tip is dithered laterally at its resonant frequency while the amplitude of the oscillation is monitored (28–30). …”

Section: Methodsmentioning

confidence: 99%

Near-Field Scanning Optical Microscopy for High-Resolution Membrane Studies

et al. 2012

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The desire to directly probe biological structures on the length scales that they exist has driven the steady development of various high-resolution microscopy techniques. Among these, optical microscopy and, in particular, fluorescence-based approaches continue to occupy dominant roles in biological studies given their favorable attributes. Fluorescence microscopy is both sensitive and specific, is generally noninvasive toward biological samples, has excellent temporal resolution for dynamic studies, and is relatively inexpensive. Light-based microscopies can also exploit a myriad of contrast mechanisms based on spectroscopic signatures, energy transfer, polarization, and lifetimes to further enhance the specificity or information content of a measurement. Historically, however, spatial resolution has been limited to approximately half the wavelength due to the diffraction of light. Near-field scanning optical microscopy (NSOM) is one of several optical approaches currently being developed that combines the favorable attributes of fluorescence microscopy with superior spatial resolution. NSOM is particularly well suited for studies of both model and biological membranes and application to these systems is discussed.

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Section: Methodsmentioning

confidence: 99%

Near-Field Scanning Optical Microscopy for High-Resolution Membrane Studies

et al. 2012

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“…The long decay time is a severe limit on scanning speed of the system. From these observations, it was concluded that severe topography and cell motion would prevent the wide application of shear-force imaging of live specimens, and that sample preparation will be the key to the success of the imaging process 2 The use of the combined SNOM/AFM (SNOAM) system for studying biological materials in aqueous solutions have also been conducted, suggesting that the system is widely applicable to specimens in water and other fluid media ". To reduce the possible damage to the probe and the soft specimens, the cantilever scanning was controlled by dynamic mode AFM .…”

Section: Snom/afm (Snoam)mentioning

confidence: 96%

<title>Scanning near-field optical microscopy: application to biological sciences</title>

Lim

2001

SPIE Proceedings

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Recent developments in genetic engineering and medical informatics offer enormous potential for biotechnology. However, key enabling technologies, such as medical instrumentation and analytical tools, are required to support further research in this field. The scanning near-field optical microscopy (SNOM) is one of the key instruments for research in these areas. In this paper, we review the synergy of the SNOM with other technologies for the imaging and characterization of biological materials. Based on this review, the components and systems design parameters are summarized.

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“…Biological objects are soft samples and three dimensionally extended with large height modulations (typically in the range of some µm). These height modulations can limit the scan rate [57] and might cause interpretation problems since a strong coupling between topographic and optical imaging is not always preserved [2,21,25]. Recent estimates [4], however, have shown that also for biological samples near-field light microscopy with a spatial resolution in the sub-hundred nano-metre range becomes feasible.…”

Section: Introductionmentioning

confidence: 99%

Variations in Cell Surfaces of Estrogen Treated Breast Cancer Cells Detected by A Combined Instrument for Far‐Field and Near‐Field Microscopy

Perner¹,

Rapp²,

Dressler

et al. 2002

Analytical Cellular Pathology

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The response of single breast cancer cells (cell line T‐47D) to 17β‐estradiol (E2) under different concentrations was studied by using an instrument that allows to combine far‐field light microscopy with high resolution scanning near‐field (AFM/SNOM) microscopy on the same cell. Different concentrations of E2 induce clearly different effects as well on cellular shape (in classical bright‐field imaging) as on surface topography (atomic force imaging) and absorbance (near‐field light transmission imaging). The differences range from a polygonal shape at zero via a roughly spherical shape at physiological up to a spindle‐like shape at un‐physiologically high concentrations. The surface topography of untreated control cells was found to be regular and smooth with small overall height modulations. At physiological E2 concentrations the surfaces became increasingly jagged as detected by an increase in membrane height. After application of the un‐physiological high E2 concentration the cell surface structures appeared to be smoother again with an irregular fine structure. The general behaviour of dose dependent differences was also found in the near‐field light transmission images. In order to quantify the treatment effects, line scans through the normalised topography images were drawn and a rate of co‐localisation between high topography and high transmission areas was calculated. The cell biological aspects of these observations are, so far, not studied in detail but measurements on single cells offer new perspectives to be empirically used in diagnosis and therapy control of breast cancers.

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Biological near-field scanning optical microscopy: instrumentation and sample issues for shear-force feedback

Cited by 12 publications

References 11 publications

Near-Field Scanning Optical Microscopy for High-Resolution Membrane Studies

Near-Field Scanning Optical Microscopy for High-Resolution Membrane Studies

<title>Scanning near-field optical microscopy: application to biological sciences</title>

Variations in Cell Surfaces of Estrogen Treated Breast Cancer Cells Detected by A Combined Instrument for Far‐Field and Near‐Field Microscopy

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