Biological Pretreatment of Rubberwood withCeriporiopsis subvermisporafor Enzymatic Hydrolysis and Bioethanol Production

Abstract

Rubberwood (Hevea brasiliensis), a potential raw material for bioethanol production due to its high cellulose content, was used as a novel feedstock for enzymatic hydrolysis and bioethanol production using biological pretreatment. To improve ethanol production, rubberwood was pretreated with white rot fungus Ceriporiopsis subvermispora to increase fermentation efficiency. The effects of particle size of rubberwood (1 mm, 0.5 mm, and 0.25 mm) and pretreatment time on the biological pretreatment were first determined by chemical analysis and X-ray diffraction and their best condition obtained with 1 mm particle size and 90 days pretreatment. Further morphological study on rubberwood with 1 mm particle size pretreated by fungus was performed by FT-IR spectra analysis and SEM observation and the result indicated the ability of this fungus for pretreatment. A study on enzymatic hydrolysis resulted in an increased sugar yield of 27.67% as compared with untreated rubberwood (2.88%). The maximum ethanol concentration and yield were 17.9 g/L and 53% yield, respectively, after 120 hours. The results obtained demonstrate that rubberwood pretreated by C. subvermispora can be used as an alternative material for the enzymatic hydrolysis and bioethanol production.

Mechanical behaviour of acetylated rubber wood subjected to artificial weathering

“…This peak range was chosen as it is least affected by chemical modification and weathering. For unmodified rubber wood, IR absorbance peaks in the fingerprint region between 800 and 1800 cm −1 were assigned to functional groups from wood biopolymers according to literature (Harrington et al 1964;Hon 1989;Pandey 1999;Pandey and Pitman 2002;Gierlinger et al 2008;Le Troedec et al 2008;Nazarpour et al 2013).…”

Bioethanol Production from Lignocellulosic Biomass

“…have been cultivated on lignocellulose with expectation of utilizing the metabolism of the microorganism to destruct the crystallinity of cellulose and remove lignin. However, most studies were based on laboratory scales and showed limited results [26][27][28][29].…”

POTENSI PEMANFAATAN LIGNOSELULOSA DARI BIOMASA KAYU KARET (Hevea brasisiliensis Muell Arg.)

“…Studi selanjutnya menggunakan Saccharomyces cerevisiae, efisiensi hidrolisis mencapai 80%, 85% dan 90% masing-masing untuk kelapa sawit, kayu karet dan campuran kayu keras ' (Chin et al, 2011). Selain Saccharomyces cerevisiae, mikroba jenis jamur pelapuk putih juga cukup potensial untuk perlakuan pendahuluan kayu karet secara biologi, seperti Ceriporiopsis subvermispora dan Trametes versicolor (Nazarpour et al, 2013). Pengembangan bahan yang mengandung lignoselulosa untuk produksi bioetanol masih menemui beberapa kendala.…”