Biological Production of Butanol and Higher Alcohols

Zhao, Jun; Lu, Congcong; Chen, Chih‐Chin; Yang, Shang‐Tian

doi:10.1002/9781118642047.ch13

Cited by 25 publications

(22 citation statements)

References 183 publications

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“…Butanol production via acetone‐butanol‐ethanol (ABE) fermentation by solventogenic clostridia such as Clostridium acetobutylicum and Clostridium beijerinckii usually suffers from low butanol yield, titer, and productivity (Ezeji et al, ; Lee et al, ; Xue et al, ; Zhao et al, ). Solventogenic clostridia are also difficult to manipulate because of the complexities in their gene regulation and metabolic pathways (Lutke‐Eversloh, ; Papoutsakis, ).…”

Section: Introductionmentioning

confidence: 99%

Metabolic process engineering of Clostridium tyrobutyricum Δack–adhE2 for enhanced n‐butanol production from glucose: Effects of methyl viologen on NADH availability, flux distribution, and fermentation kinetics

Jiang

Yu³

et al. 2014

Biotech & Bioengineering

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Butanol biosynthesis through aldehyde/alcohol dehydrogenase (adhE2) is usually limited by NADH availability, resulting in low butanol titer, yield, and productivity. To alleviate this limitation and improve n-butanol production by Clostridium tyrobutyricum Δack-adhE2 overexpressing adhE2, the NADH availability was increased by using methyl viologen (MV) as an artificial electron carrier to divert electrons from ferredoxin normally used for H2 production. In the batch fermentation with the addition of 500 μM MV, H2 , acetate, and butyrate production was reduced by more than 80-90%, while butanol production increased more than 40% to 14.5 g/L. Metabolic flux analysis revealed that butanol production increased in the fermentation with MV because of increased NADH availability as a result of reduced H2 production. Furthermore, continuous butanol production of ∼55 g/L with a high yield of ∼0.33 g/g glucose and extremely low ethanol, acetate, and butyrate production was obtained in fed-batch fermentation with gas stripping for in situ butanol recovery. This study demonstrated a stable and reliable process for high-yield and high-titer n-butanol production by metabolically engineered C. tyrobutyricum by applying MV as an electron carrier to increase butanol biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Metabolic process engineering of Clostridium tyrobutyricum Δack–adhE2 for enhanced n‐butanol production from glucose: Effects of methyl viologen on NADH availability, flux distribution, and fermentation kinetics

Jiang

Yu³

et al. 2014

Biotech & Bioengineering

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“…Similar amounts of butanol, ethanol and acetate but slightly more butyrate (5.0 g/L) were produced from sucrose in the fed-batch fermentation, which stopped at 56 h with ~21.0 g/L sucrose unused (Fig. 2B), probably because of butanol inhibition (Zhao et al, 2013). The butanol yield and productivity obtained in these fermentations were 0.31 ± 0.02 g/g and 0.33 ± 0.02 g/L•h, respectively.…”

Section: Free-cell Fermentations In Stirred-tank Bioreactormentioning

confidence: 82%

“…In recent years, biological production of n-butanol as a renewable substitute of gasoline has attracted large attention for its higher energy density and superior fuel properties compared to ethanol (Zhao et al, 2013). However, butanol production by solventogenic clostridia in acetone-butanol-ethanol (ABE) fermentation suffered from low productivity, yield, and product titer (Zhao et al, 2013). Consequently, the high production cost of biobutanol impedes its industrial application (Green et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

n-Butanol production from sucrose and sugarcane juice by engineered Clostridium tyrobutyricum overexpressing sucrose catabolism genes and adhE2

Zhang

Meng³

et al. 2017

Bioresource Technology

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The production of n-butanol from sugarcane juice by metabolically engineered Clostridium tyrobutyricum Ct(Δack)-pscrBAK overexpressing scr operon genes (scrB, scrA, and scrK) for sucrose catabolism and an aldehyde/alcohol dehydrogenase gene (adhE2) for butanol biosynthesis was studied with corn steep liquor (CSL) as a low-cost nitrogen source. In free cell fermentation, butanol production of ∼16g/L at a yield of 0.31±0.02g/g and productivity of 0.33±0.02g/L·h was obtained from sucrose and yield of 0.24±0.02g/g and productivity of 0.30±0.01g/L·h from sugarcane juice containing sucrose, glucose and fructose. The fermentation was also studied in a fibrous bed bioreactor (FBB) operated in a repeated batch mode for 10 consecutive cycles in 10days, achieving an average butanol yield of 0.21±0.02g/g and productivity of 0.53±0.05g/L·h from sugarcane juice, demonstrating its long-term stability without applying the antibiotic selection pressure.

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“…Biological production of n‐butanol, an important industrial solvent and potentially an advanced biofuel, from lignocellulosic biomass in acetone–butanol–ethanol (ABE) fermentation by solvent‐producing Clostridium acetobutylicum and Clostridium beijerinckii has drawn increasing attentions in recent years (Green, ; Jang et al, ; Wang et al, ). However, conventional ABE fermentation with the biphasic metabolic shift from acidogenesis to solventogenesis is difficult to control and is prone to “acid crash” due to the complex metabolic regulation in solventogenic Clostridium (Xu et al, ; Zhao et al, ). Recently, we have engineered Clostridium tyrobutyricum , an acidogen natively producing acetate and butyrate as two major fermentation products from glucose and xylose (Liu et al, ), to produce n‐butanol by overexpressing adhE2 encoding a bifunctional aldehyde/alcohol dehydrogenase (Yu et al, ).…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering of Clostridium tyrobutyricum for n‐butanol production through co‐utilization of glucose and xylose

Tang³

et al. 2015

Biotech & Bioengineering

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The glucose-mediated carbon catabolite repression (CCR) in Clostridium tyrobutyricum impedes efficient utilization of xylose present in lignocellulosic biomass hydrolysates. In order to relieve the CCR and enhance xylose utilization, three genes (xylT, xylA, and xylB) encoding a xylose proton-symporter, a xylose isomerase and a xylulokinase, respectively, from Clostridium acetobutylicum ATCC 824 were co-overexpressed with aldehyde/alcohol dehydrogenase (adhE2) in C. tyrobutyricum (Δack). Compared to the strain Ct(Δack)-pM2 expressing only adhE2, the mutant Ct(Δack)-pTBA had a higher xylose uptake rate and was able to simultaneously consume glucose and xylose at comparable rates for butanol production. Ct(Δack)-pTBA produced more butanol (12.0 vs. 3.2 g/L) with a higher butanol yield (0.12 vs. 0.07 g/g) and productivity (0.17 vs. 0.07 g/L · h) from both glucose and xylose, while Ct(Δack)-pM2 consumed little xylose in the fermentation. The results confirmed that the CCR in C. tyrobutyricum could be overcome through overexpressing xylT, xylA, and xylB. The mutant was also able to co-utilize glucose and xylose present in soybean hull hydrolysate (SHH) for butanol production, achieving a high butanol titer of 15.7 g/L, butanol yield of 0.24 g/g, and productivity of 0.29 g/L · h. This study demonstrated the potential application of Ct(Δack)-pTBA for industrial biobutanol production from lignocellulosic biomass.

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Biological Production of Butanol and Higher Alcohols

Cited by 25 publications

References 183 publications

Metabolic process engineering of Clostridium tyrobutyricum Δack–adhE2 for enhanced n‐butanol production from glucose: Effects of methyl viologen on NADH availability, flux distribution, and fermentation kinetics

Metabolic process engineering of Clostridium tyrobutyricum Δack–adhE2 for enhanced n‐butanol production from glucose: Effects of methyl viologen on NADH availability, flux distribution, and fermentation kinetics

n-Butanol production from sucrose and sugarcane juice by engineered Clostridium tyrobutyricum overexpressing sucrose catabolism genes and adhE2

Metabolic engineering of Clostridium tyrobutyricum for n‐butanol production through co‐utilization of glucose and xylose

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