Biological properties and viral surface antigens of Burkitt lymphoma- and mononucleosis- derived strains of Epstein-Barr virus released from transformed marmoset cells

Miller, George; Coope, D; Niederman, James C.; Pagano, J. S.

doi:10.1128/jvi.18.3.1071-1080.1976

Cited by 71 publications

(34 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The development of a monoclonal antibody specific for the HTLV protein p19 and its use in an immunofluorescence assay has recently been described (Robert-Guroff et al, 1981). A series of xenoantigens specifically recognized by murine monoclonal antibodies in either ascites fluid or tissue culture fluid were detected by indirect membrane immunofluorescence using murine monoclonal antibodies as the primary reagents and a FITC-conjugated goat antimouse IgG preparation as the secondary reagent as previously described (Tsubota et al, 1977;Minowada et al, 1981). Detailed preparation and specificity of most of the monoclonal antibodies have been previously reported (Minowada et al, 1981) particularily for the OKT Reinherz et al, 1979) and Leu (Warnke et al, 1980;Ledbetter et al, 1981) series of antibodies.…”

Section: Immunofluorescence Assaysmentioning

confidence: 99%

Persistent in vitro infection by human T‐cell leukemia‐lymphoma virus (HTLV) of normal human T‐lymphocytes from blood relatives of patients with HTLV‐associated mature T‐cell neoplasms

Ruscetti¹,

Robert-Guroff²,

Ceccherini‐Nelli³

et al. 1983

Intl Journal of Cancer

View full text Add to dashboard Cite

Human T-cell lymphoma-leukemia virus (HTLV), a novel type-C retrovirus, has been identified in tumor cells of patients with mature T-cell neoplasms. Attempts to transmit the first isolate of HTLV designated HTLV-I , , , to secondary cultures of animal cells and several attempts to transmit HTLV t o normal human T cells, activated with PHA and grown with T-cell growth factor (TCGF), obtained from randomly selected normal adult donors were unsuccessful. Peripheral blood leukocytes were obtained from blood relatives of patients with HTLV-positive T-cell neoplasms. These normal adult T cells were exposed to HTLV, and in 5 of 10 samples tested from three separate families, persistent infection of cultured Tlymphocytes by HTLV occurred. HTLV infection was monitored by three methods: an indirect membrane immunofluorescence assay for HTLV p19; an in situ hybridization assay for intracellular viral RNA; and electron microscopic analysis for extracellular viral particles. Two of the five infected T-cell lines have been continuously positive for HTLV for over 24 months in continuous culture. B-cell lines from the same individuals could not be infected by HTLV-I Despite the release of viral particles from the infectedc&ures detected by electron microscopy, no extracellular reverse transcriptase was found. HTLV infection stimulated increased long-term growth of the T cells although all HTLV-l(c,)-infected cultured cells always remained dependent on the addition of TCGF for growth. Phenotypic characterization of the infected cells by monoclonal antibodies, HLA typing, cytochemical and cell surface markers showed that all the infected cells were mature T cells and were easily distinguished from the cells used as a source of HTLV for infection. The infected cells were either of a predominantly helper or of a suppressor phenotype showing that both mature T-cell subsets can be infected. Whether these data indicate that there is a genetic basis for susceptibility of human T cells to HTLV infection or reflect an increased sensitivity of some cells to infection by low titers of HTLV remains to be determined. These results provide further evidence for a predominant T-cell tropism of HTLV.

show abstract

Section: Immunofluorescence Assaysmentioning

confidence: 99%

Persistent in vitro infection by human T‐cell leukemia‐lymphoma virus (HTLV) of normal human T‐lymphocytes from blood relatives of patients with HTLV‐associated mature T‐cell neoplasms

Ruscetti¹,

Robert-Guroff²,

Ceccherini‐Nelli³

et al. 1983

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…Lymphocytes from the cord blood of five different fetuses (1509 and 1522, 1523, 1524 and 1525) were separated similarly by density gradient centii-lymphoma cells (Miller et al, 1976). fugation and immortalized by EBV infection in vitro as described elsewhere (Nilsson, 1976).…”

Section: Cell Linesmentioning

confidence: 99%

Growth of diploid, epstein‐barr virus‐carrying human lymphoblastoid cell lines heterotransplanted into nude mice under immunologically privileged conditions

Giovanella

Nilsson

Zech

et al. 1979

Intl Journal of Cancer

View full text Add to dashboard Cite

Human Epstein-Barr virus-carrying lymphoid cell lines which have been classified on the basis of studies on clonality and morphological, chromosomal and functional parameters as lymphoblastoid cell lines (LCL) of presumed non-neoplastic origin were inoculated intracerebrally into nude mice. All eighteen of them grew, killing the host mice within 7 to 25 days, except for 2 which grew more slowly. At autopsy, the brain of the nudes was found to be invaded by infiltrating lymphomas. Sixteen of these lymphomas, when recultured in vitro, gave rise to cell lines with growth properties and morphology indistinguishable from those of the inoculated LCL. Chromosomal examinations showed that 3/7 cell lines injected, which grew as lymphomas in the brain, were still normal diploid on reexplantation whereas the remaining four had become aneuploid. Four lines derived from intracerebral lymphomas (2 diploid, 1 aneuploid and 1 untested) were inoculated subcutaneously into adult nude mice. None of them grew. When the corresponding four original LCL lines were inoculated subcutaneously into newborn nude mice, they grew rapidly, but failed to do so in newborn normal mice or intracerebrally in adult normal mice. One such line, U-1450, was treated with anti-lymphocyte serum (ALS). Small nodules developed at the site of inoculation. From one nodule a cell line was cultured, 1450 ALSAD. It was morphologically indistinguishable from the line of origin. The lines obtained from nude mice inoculated with polyclonal LCL seem to have a restricted clonal representation, but were not monoclonal, as evidenced by analyses of their pattern of immunoglobulin synthesis.

show abstract

“…ble relationship of strain variation to the different diseases with which EBV has been associated, attention has focused on analysis of differences between isolates of EBV (3,13,18,25). Quantities of virus, adequate for chemical analyses, can be obtained from three sources: from a Burkitt tumor cell line which has been grown in culture (the HR-1 line) (10), from marmoset lymphocytes infected in vitro with an infectious mononucleosis isolate (B95-8) (21), and from marmoset lymphocytes infected with another (Nyevu) Burkitt tumor isolate (W91) (18). Virus from the HR-1 line, EBV HR-1, can be used to superinfect Raji cells, thereby inducing the synthesis of early antigen (9), but this virus cannot transform normal lymphocytes to cells possessing capacity for long-term growth in vitro (17, VOL.…”

mentioning

confidence: 99%

DNA of Epstein-Barr virus. III. Identification of restriction enzyme fragments that contain DNA sequences which differ among strains of Epstein-Barr virus

Raab‐Traub¹,

Pritchett²,

Kieff³

1978

J Virol

View full text Add to dashboard Cite

Previous kinetic and absorption hybridization experiments had demonstrated that the DNA of the B95-8 strain of Epstein-Barr virus was missing approximately 10% of the DNA sequences present in the DNA of the HR-1 strain (experiments reported here, the restriction enzyme fragments of Epstein-Barr virus DNA which contain sequences which differ among the HR-1, B95-8, and W91 strains have been identified. The DNA of the HR-1, B95-8, and W91 strains each differed in complexity. The sequences previously shown to be missing in the B95-8 strain were contained in the EcoRI-C and -D and Hsu I-E and -N fragments of the HR-1 strain and in the EcoRI-C and Hsu I-D and -E fragments of the W91 strain. The HR-1 strain was missing DNA contained in EcoRI fragments A and J through K and Hsu I fragment B of the B95-8 strain and in the EcoRI-A and Hsu I-B fragments of the W91 strain. The relationship of these data to the linkage map of restriction enzyme fragments of the DNA of the B95-8 and W91 strains

show abstract

Biological properties and viral surface antigens of Burkitt lymphoma- and mononucleosis- derived strains of Epstein-Barr virus released from transformed marmoset cells

Cited by 71 publications

References 19 publications

Persistent in vitro infection by human T‐cell leukemia‐lymphoma virus (HTLV) of normal human T‐lymphocytes from blood relatives of patients with HTLV‐associated mature T‐cell neoplasms

Persistent in vitro infection by human T‐cell leukemia‐lymphoma virus (HTLV) of normal human T‐lymphocytes from blood relatives of patients with HTLV‐associated mature T‐cell neoplasms

Growth of diploid, epstein‐barr virus‐carrying human lymphoblastoid cell lines heterotransplanted into nude mice under immunologically privileged conditions

DNA of Epstein-Barr virus. III. Identification of restriction enzyme fragments that contain DNA sequences which differ among strains of Epstein-Barr virus

Contact Info

Product

Resources

About