“…Aer 25 days of biological activity, a suitable biolm layer was formed on the media. [19][20][21] It should be noted that the hydraulic retention time (HRT) under continuous conditions was considered 24 h. Aer the growth of biolm and compatibility of microorganisms with 2,4-D herbicide, different concentrations (1.0, 5.0, 10.0, 20.0, 30.0, 35.0, 40.0, 50.0 and 60.0 volume percentage of synthetic wastewater) solution containing 2,4-D herbicide releasing from the 3DE process was added to synthetic wastewater for adaption of microorganisms to the 2,4-D herbicide. The biological process was set up with a lling ratio of 70% and a retention time of 24 h, and at different retention times (8, 12 and 24 h), the reactor efficiency was measured for this lling ratio (70%).…”