Biological responses to core–shell-structured Fe₃O₄@SiO₂-NH₂ nanoparticles in rats by a nuclear magnetic resonance-based metabonomic strategy

Abstract

BackgroundCore–shell-structured nanoparticles (NPs) have attracted much scientific attention due to their promising potential in biomedical fields in recent years. However, their underlying mechanisms of action and potential adverse effects following administration remain unknown.MethodsIn the present study, a 1H nuclear magnetic resonance-based metabonomic strategy was applied to investigate the metabolic consequences in rats following the intravenous administration of parent NPs of core–shell-structured nanoparticles, Fe3O4@SiO2-NH2 (Fe@Si) NPs.ResultsAlterations reflected in plasma and urinary metabonomes indicated that Fe@Si NPs induced metabolic perturbation in choline, ketone-body, and amino-acid metabolism besides the common metabolic disorders in tricarboxylic acid cycle, lipids, and glycogen metabolism often induced by the exogenous agents. Additionally, intestinal flora metabolism and the urea cycle were also influenced by Fe@Si NP exposure. Time-dependent biological effects revealed obvious metabolic regression, dose-dependent biological effects implied different biochemical mechanisms between low- and high-dose Fe@Si NPs, and size-dependent biological effects provided potential windows for size optimization.ConclusionNuclear magnetic resonance-based metabonomic analysis helps in understanding the biological mechanisms of Fe@Si NPs, provides an identifiable ground for the selection of view windows, and further serves the clinical translation of Fe@Si NP-derived and -modified bioprobes or bioagents.

Determination of aflatoxin B1 in Pixian Douban based on aptamer magnetic solid-phase extraction

“…The Si-O-Si group exhibited a strong absorption intensity at 1100.21 cm À1 owing to the silica coating, indicating that Fe 3 O 4 had been successfully coated with SiO 2 . 36 In addition, the Absorption peaks at 1627.94 and 3328.89 cm À1 correspond to bending and stretching vibrations of the-NH 2 group, 16 respectively. The peaks at 2856.35 and 2923.24 cm À1 correspond to symmetric and asymmetric stretching vibrations of the -CH 2 group, respectively, which are attributed to the hydrolysis of the amination reagent APTES.…”

“…Ferrosoferric oxide (Fe 3 O 4 ) is widely used in sample pretreatment because of its characteristics of magnetic adsorption, low price, easy synthesis, and surface modication. [15][16][17] Nevertheless, Fe 3 O 4 is unstable and easily oxidized by air. Surface modication of Fe 3 O 4 with appropriate functional groups can prevent oxidation and improve its durability and adsorption.…”

A Nanosensor for Naked-Eye Identification and Adsorption of Cadmium Ion Based on Core–Shell Magnetic Nanospheres

“…Magnetic Fe 3 O 4 @SiO 2 nanoparticles with a core–shell structure have attracted an increasing interest in biological and environmental fields [ 11 , 12 , 13 , 14 ]. The particles with nano size possess abundant surface active sites for surface modification, and the greatest strength of Fe 3 O 4 @SiO 2 nanoparticles is their targeting ability.…”