Biological role of 8-oxo-2'-deoxyguanosine

Marmiy, N. V.; Esipov, D. S.

doi:10.3103/s0096392515040070

Cited by 10 publications

(7 citation statements)

References 30 publications

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“…It may be the result of ROS synthesis induction in that cells, or possibly, the oxidized DNA accumulates in them. First, it is believed that the oxidative deoxyguanosine modifications in DNA are exclusively genotoxic and mutagenic and, therefore, harm nucleic acids [50, 51]; second, it is assumed that easily oxidized G in the cfDNA composition may act as the intracellular antioxidant [41].…”

Section: Discussionmentioning

confidence: 99%

Oxidized Cell-Free DNA Role in the Antioxidant Defense Mechanisms under Stress

Filev

Shmarina

Ershova

et al. 2019

Oxidative Medicine and Cellular Longevity

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The present study focuses on the investigation of the oxidized cell-free DNA (cfDNA) properties in several experimental models, including cultured cerebellum cells, peripheral blood lymphocytes (PBL), plasma, and hippocampus under an acute and chronic unpredictable stress model in rats. Firstly, our study shows that Spectrum Green fluorescence-labeled oxidized cfDNA fragments were transferred into the cytoplasm of 80% of the cerebellum culture cells; meanwhile, the nonoxidized cfDNA fragments do not pass into the cells. Oxidized cfDNA stimulates the antioxidant mechanisms and induction of transcription factor NRF2 expression, followed by an activation of NRF2 signaling pathway genes—rise of Nrf2 and Hmox1 gene expression and consequently NRF2 protein synthesis. Secondly, we showed that stress increases plasma cfDNA concentration in rats corresponding with the duration of the stress exposure. At the same time, our study did not reveal any significant changes of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) level in PBL of rats under acute or chronic stress, probably due to the significantly increased Nrf2 expression, that we found in such conditions. 8-oxodG is one of the most reliable markers of DNA oxidation. We also found an increased level of 8-oxodG in the hippocampal homogenates and hippocampal dentate gyrus in rats subjected to acute and chronic stress. Taken together, our data shows that oxidized cfDNA may play a significant role in systemic and neuronal physiological mechanisms of stress and adaptation.

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Section: Discussionmentioning

confidence: 99%

Oxidized Cell-Free DNA Role in the Antioxidant Defense Mechanisms under Stress

Filev

Shmarina

Ershova

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Changes in genetic information, mutagenesis, and cellular apoptosis associated with this make oxidative damage of nucleic acids one of the most dangerous modifications among biomolecules [5]. It was previously shown that of the four bases, which make up the DNA structure, guanine is the most oxidized [6]. Among the most important biological OS markers there are the following purines: 8-hydroxy-2'-deoxyguanosine (8-OHdG) or its oxidized form 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG).…”

Section: Introductionmentioning

confidence: 99%

“…Among the most important biological OS markers there are the following purines: 8-hydroxy-2'-deoxyguanosine (8-OHdG) or its oxidized form 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG). 8-OHdG is considered as the most sensitive and useful marker of oxidative DNA damage [7,8], which can be found in many tissues and fluids as it easily penetrates from cells into the blood [6]. However, the biological role of 8-OHdG is currently poorly understood [6,9].…”

Section: Introductionmentioning

confidence: 99%

Role of plasma 8-OXO-2’-deoxyguanosine in target organ damage in patients with hypertension and type 2 diabetes

Nemtsova

Shalimova

Kolesnikova

et al. 2022

Arterial Hypertension

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“…В последнее десятилетие появились исследования, сообщающие о вероятной значимой биологической роли 8-oxo-dG [5] и открывающие перспективы применения его в качестве лекарственного препарата. В частности, были показаны выраженные противовоспалительный [6] и иммунорегулирующий [7] эффекты этого соединения, а также выдвинута гипотеза о его важной роли в регуляции репарации ДНК и ее сопряжении с внутриклеточной сигнализацией [8].…”

Section: Introductionunclassified

Method of Preparative Synthesis of 8-Oxo-2'-Deoxyguanosine for Laboratory Using

Marmiy

Esipov

2017

Fine Chem. Tech.

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A preparative method for the synthesis of 8-oxo-2'-deoxyguanosine (8-oxo-dG) giving a high yield up to 80% and suitable for laboratory use is suggested. The urgency of the development of this method is associated with the need to obtain large quantities of 8-oxo-dG for the study of biological and pharmaceutical activity. Evidence of this need was obtained in the last decades, when the mechanisms of the interface of DNA repair with intracellular signaling through 8-oxo-dG were discovered and its anti-inflammatory and protective effects were revealed. The suggested method is based on the scheme used to prepare 8-oxo-dG-containing oligonucleotides, but has a number of important modifications. The use of N,N-dimethylformamide as a solvent makes it possible to increase the yield of the nucleophilic substitution reaction and avoid expensive and complicated further purification of silver acetate. Control of the reaction kinetics allows achieving the maximum yield and purity of the product. Alkaline hydrolysis, in contrast to ammonolysis, provides complete and rapid removal of acyl groups, and reversed-phase chromatography on silanized silica gel is an effective and inexpensive method of purification. Such a purification scheme minimizes contamination of the product with residual organic solvents, which is important for biological experiments. From the economic point of view, it is advantageous because of the absence of expensive solvents and the possibility of reusing the carrier. The method makes it possible to obtain pure 8-oxo-dG in preparative amounts and exceeds both its prototype and other existing techniques in the final output. Some intermediate compounds and significant methodological features of the reaction are described.

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Biological role of 8-oxo-2'-deoxyguanosine

Cited by 10 publications

References 30 publications

Oxidized Cell-Free DNA Role in the Antioxidant Defense Mechanisms under Stress

Oxidized Cell-Free DNA Role in the Antioxidant Defense Mechanisms under Stress

Role of plasma 8-OXO-2’-deoxyguanosine in target organ damage in patients with hypertension and type 2 diabetes

Method of Preparative Synthesis of 8-Oxo-2'-Deoxyguanosine for Laboratory Using

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