Biological Sample Printing

Bigdelou, Parnian; Roth, Alexander; Datar, Akshata; Lee, Moo‐Yeal

doi:10.1007/978-3-319-46805-1_4

Cited by 2 publications

(3 citation statements)

References 16 publications

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“…For solenoid valve-based spotters, the sample viscosity should not exceed 30 cP (such as e.g. a 1% (m/v) alginate solution) [65]. Considering the limited benefit of hydrogels with valve-based spotter, we optimized the ambient parameters (temperature and humidity) to avoid evaporation.…”

Section: Discussionmentioning

confidence: 99%

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Development of bone cell microarrays in microfluidic chips for studying osteocyte-osteoblast communication under fluid flow mechanical loading

Yvanoff¹,

Willaert²

2022

Biofabrication

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Bone tissue remodels throughout life in response to mechanical loads. Impaired activities of bone cells (osteocytes, osteoblasts and osteoclasts) result in a disruption of the bone remodelling cycle, which eventually leads to bone disorders such as osteoporosis. To develop efficient therapeutic strategies against bone disorders, new tools are needed to unravel the bone remodelling cycle at the molecular level. Here, we developed a microfluidic platform, which should allow understanding the bone remodelling cycle in much more detail and ultimately be used to discover new therapeutic compounds. We focused specifically on studying cell-cell communication between osteocytes and osteoblasts cells via connexin 43-gap junctions. Therefore, a new cell printing method was developed to create living cellular bone cell arrays in a microfluidic channel. Several cell printing designs where osteocytes and osteoblasts heterotypically interacted at localized interfaces were evaluated. Physical contacts between the bone cells were characterised at high resolution by correlative atomic force microscopy (AFM) - fluorescence microscopy. We demonstrated that the platform is compatible with single-cell mechanostimulation by AFM nanoindentation and subsequent fluorescent analysis of the mechanoresponse. As a proof of concept, we showed the functionality of the platform by analysing the induced in vivo-like Ca++ wave in the printed osteocyte-osteoblast network upon mechanical stimulation by fluid flow shear stress.

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Section: Discussionmentioning

confidence: 99%

“…the expression of a fluorescentlytagged reporter introduced by reverse transfection [70][71][72][73]. Of interest, it was reported that cell densities higher than 1 × 10 7 cells ml −1 could lead to clogging of the solenoid valve [52,65].…”

Section: Discussionmentioning

confidence: 99%

Development of bone cell microarrays in microfluidic chips for studying osteocyte-osteoblast communication under fluid flow mechanical loading

Yvanoff¹,

Willaert²

2022

Biofabrication

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“…Today, computational methods receive a lot more attention from researchers than before. This is mainly due to the much lower costs and relatively acceptable results of these methods in comparison to experimental techniques in different aspects of mechanical engineering, such as enhanced oil recovery and new energies [1][2][3][4], biomass gasification [5][6][7][8][9][10][11][12] and pyrolysis [13][14][15][16][17][18][19][20][21][22], chemical reactions [23][24][25][26], molecular dynamics [27,28], smoothed particle hydrodynamics [29], and nanofluid applications [30][31][32]. An important aspect of mechanical engineering that needs to be investigated in detail is the combustion of solid particles.…”

Section: Introductionmentioning

confidence: 99%

Analytical Simulation of the Cloud Combustion of Corn Starch Microparticles

et al. 2019

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Knowledge of the fundamentals of dust combustion is necessary for designing safety technologies such as flame arrestors. The structure of flame propagation in a uniformly distributed cloud of volatile fuel particles was simulated. A model based on energy and mass conservation laws was generated. The obtained system of equations was nondimensionalized and solved according to the introduced boundary conditions. Comparison of the results with the existing experimental data in the literature showed remarkable agreement. Finally, the effect of parameters such as particle size and Lewis number on the characteristics of the cloud combustion of biomass microparticles under both lean and rich conditions was studied.

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Biological Sample Printing

Cited by 2 publications

References 16 publications

Development of bone cell microarrays in microfluidic chips for studying osteocyte-osteoblast communication under fluid flow mechanical loading

Development of bone cell microarrays in microfluidic chips for studying osteocyte-osteoblast communication under fluid flow mechanical loading

Analytical Simulation of the Cloud Combustion of Corn Starch Microparticles

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