Biological studies using mammalian cell lines and the current status of the microbeam irradiation system, SPICE

Konishi, Teruaki; Ishikawa, Takahiro; Iso, Hiroyuki; Yasuda, N.; Oikawa, Masakazu; Higuchi, Yuichi; Kato, Takamitsu A.; Hafer, Kurt; Kodama, Kumiko; Hamano, Tsuyoshi; Suya, Noriyoshi; Imaseki, Hitoshi

doi:10.1016/j.nimb.2009.03.060

Cited by 16 publications

(6 citation statements)

References 31 publications

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“…To be able to control and accurately quantify such low doses of the high-LET-radiation, it seems necessary to switch from the radioactive source to microbeam facilities. 19) In the present study, a microbeam irradiation system (Single-Particle Irradiation System to Cell, acronym as SPICE) 20) at the National Institute of Radiological Sciences (NIRS), Japan, was employed for the priming irradiation. The use of microbeam irradiation techniques provides a unique opportunity to control precisely the number of particles traversing individual cells and localization of dose within the cells.…”

Section: Introductionmentioning

confidence: 99%

Adaptive Response in Zebrafish Embryos Induced Using Microbeam Protons as Priming Dose and X-ray Photons as Challenging Dose

Choi

Konishi²,

Oikawa³

et al. 2010

JRR

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In the studies reported here, a high-linear-energy-transfer (high-LET)-radiation dose was used to induce adaptive response in zebrafish embryos in vivo. Microbeam protons were used to provide the priming dose and X-ray photons were employed to provide the challenging dose. The microbeam irradiation system (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), Japan, was employed to control and accurately quantify the number of protons at very low doses, viz., about 100 µGy. The embryos were dechorionated at 4 h post fertilization (hpf) and irradiated at 5 hpf by microbeam protons. For each embryo, ten irradiation points were arbitrarily chosen without overlapping with one another. To each irradiation point, 5, 10 or 20 protons each with an energy of 3.4 MeV were delivered. The embryos were returned back to the incubator until 10 hpf to further receive the challenging exposure, which was achieved using 2 Gy of X-ray irradiation, and then again returned to the incubator until 24 hpf for analyses. The levels of apoptosis in zebrafish embryos at 25 hpf were quantified through terminal dUTP transferase-mediated nick end-labeling (TUNEL) assay, with the apoptotic signals captured by a confocal microscope. The results revealed that 5 to 20 protons delivered at 10 points each on the embryos, or equivalently 110 to 430 µGy, could induce radioadaptive response in the zebrafish embryos in vivo.

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Section: Introductionmentioning

confidence: 99%

Adaptive Response in Zebrafish Embryos Induced Using Microbeam Protons as Priming Dose and X-ray Photons as Challenging Dose

Choi

Konishi²,

Oikawa³

et al. 2010

JRR

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“…The most of in-air microbeam configurations are therefore based on direct extraction of previously focused ion microbeam into the atmosphere through a thin exit foil. Protons in the energy range between 2.4 to 5 MeV are mainly used [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. Since the influence of ion microbeam lateral spread on in-air microbeam spot size decreases with the decrease of working distance (distance between the exit foil and sample), samples are often placed on the exit foil itself [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Many authors reported experimental measurements and/or modelling of the beam spot size degradation as a function of working distance for different exit foil materials and its thicknesses. Reported working distances were from 100 µm to several mm [12][13][14][15][16][17][18][19][20][21][22]. T. Tadić et al [12] estimated 96 µm in-air microbeam resolution for focused 4 MeV proton beam and a 50 µm thick polyimide foil, while the sample was placed in air, less than 100 µm from the exit foil.…”

Section: Introductionmentioning

confidence: 99%

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In-air ion beam analysis with high spatial resolution proton microbeam

Jakšić

Chokheli

Fazinić

et al. 2016

Nuclear Instruments and Methods in Physics Research Section B:

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One of the possible ways to maintain the micrometer spatial resolution while performing ion beam analysis in the air is to increase the energy of ions. In order to explore capabilities and limitations of this approach, we have tested a range of proton beam energies (2 -6 MeV) using in-air STIM (Scanning Ion Transmission Microscopy) setup. Measurements of the spatial resolution dependence on proton energy have been compared with SRIM simulation and modelling of proton multiple scattering by different approaches. Results were used to select experimental conditions in which 1 micrometer spatial resolution could be obtained.High resolution in-air microbeam could be applied for IBIC (Ion Beam Induced Charge) tests of large detectors used in nuclear and high energy physics that otherwise can not be tested in relatively small microbeam vacuum chambers.

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“…After further optimization of the beam focusing system and improvements in the stability of the bending magnets, the beam size was reduced to approximately 5 μm. In 2008, an automated cell recognition system for targeting cell nuclei in a 2.5 mm × 2.5 mm area of the cell dish was also completed [16]. Now, after additional improvements, SPICE provides a beam size of approximately 2 μm in diameter and its irradiation procedures are fully automated with high-throughput irradiation of 3000 cells in a 5 mm × 5 mm area in a single dish within 15 min after placing the cell dish on the micro-positioning stage.…”

Section: Introductionmentioning

confidence: 99%

SPICE-NIRS Microbeam: a focused vertical system for proton irradiation of a single cell for radiobiological research

Konishi¹,

Oikawa²,

Suya³

et al. 2013

Journal of Radiation Research

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The Single Particle Irradiation system to Cell (SPICE) facility at the National Institute of Radiological Sciences (NIRS) is a focused vertical microbeam system designed to irradiate the nuclei of adhesive mammalian cells with a defined number of 3.4 MeV protons. The approximately 2-μm diameter proton beam is focused with a magnetic quadrupole triplet lens and traverses the cells contained in dishes from bottom to top. All procedures for irradiation, such as cell image capturing, cell recognition and position calculation, are automated. The most distinctive characteristic of the system is its stability and high throughput; i.e. 3000 cells in a 5 mm × 5 mm area in a single dish can be routinely irradiated by the 2-μm beam within 15 min (the maximum irradiation speed is 400 cells/min). The number of protons can be set as low as one, at a precision measured by CR-39 detectors to be 99.0%. A variety of targeting modes such as fractional population targeting mode, multi-position targeting mode for nucleus irradiation and cytoplasm targeting mode are available. As an example of multi-position targeting irradiation of mammalian cells, five fluorescent spots in a cell nucleus were demonstrated using the γ-H2AX immune-staining technique. The SPICE performance modes described in this paper are in routine use. SPICE is a joint-use research facility of NIRS and its beam times are distributed for collaborative research.

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Biological studies using mammalian cell lines and the current status of the microbeam irradiation system, SPICE

Cited by 16 publications

References 31 publications

Adaptive Response in Zebrafish Embryos Induced Using Microbeam Protons as Priming Dose and X-ray Photons as Challenging Dose

Adaptive Response in Zebrafish Embryos Induced Using Microbeam Protons as Priming Dose and X-ray Photons as Challenging Dose

In-air ion beam analysis with high spatial resolution proton microbeam

SPICE-NIRS Microbeam: a focused vertical system for proton irradiation of a single cell for radiobiological research

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