Biological studies with comprehensive 2D-GC-HRMS screening: Exploring the human sweat volatilome

Ripszam, Matyas; Bruderer, Tobias; Biagini, Denise; Ghimenti, Silvia; Lomonaco, T; Francesco, Fabio Di

doi:10.1016/j.talanta.2023.124333

Cited by 9 publications

(1 citation statement)

References 19 publications

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“…The peak areas of each chemical were calculated by integrating the corresponding retention time windows in the extracted ion images (EIIs) generated on the basis of characteristic ions of the target chemicals. Details of this workflow are described in detail in our previous work [12]. Since there was a 3:1 split ratio during trap desorption, the maximum recovery could not exceed 25%.…”

Section: Compound Recoverymentioning

confidence: 99%

Isolation of single molecules from complex GCxGC chromatograms

Ripszam,

Bruderer,

Vivaldi

et al. 2023

Preprint

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We developed a novel approach capable of isolating and recollecting nearly any molecule from complex GCxGC chromatograms. This was achieved by modifying a GCxGC-Q-TOF system with a Deans switch, a passive splitter, and careful adjustments of flows and capillary dimensions. The setup was evaluated with more than a hundred standard chemicals covering a wide range of volatility (boiling points: 56 – 343 ⁰C) and polarity (log P: -0.2 – 9.4). We found that recovery from tube to tube can become highly efficient if a custom-made adapter is attached directly on the FID port (average recovery rate of 76 ± 7%). Furthermore, we could achieve an isolation down to a minimum distance of 50 ms between baseline separated eluting peaks. In addition, the setup was designed for easy adaptation by repurposing existing instrument control software to define the isolation windows for the compounds of interest (first and second column retention time windows). We expect this novel development to allow several new applications, e.g., the isolation or selective enrichment of molecules in food and flavour analysis; the investigation of suspect chemicals (incl. unknowns) for effect directed analysis (e.g., bioassays), and the isolation of chemicals for further chemical analysis.

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Section: Compound Recoverymentioning

confidence: 99%

Isolation of single molecules from complex GCxGC chromatograms

Ripszam,

Bruderer,

Vivaldi

et al. 2023

Preprint

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Trendbericht Lebensmittelchemie 2023

Loos¹

2023

Nachr Chem

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Hören, sehen, riechen, schmecken, fühlen – mit unseren Sinnen nehmen wir Informationen über unsere Umwelt auf. So können wir die Qualität von Lebensmitteln einschätzen, aber auch – teils ohne es zu merken – mit unseren Mitmenschen kommunizieren. Gerade die Forschung zur chemischen Kommunikation beim Menschen erlebt nach Jahrzehnten einen erneuten Aufschwung in der Aroma‐ und Geruchsforschung, einem Teilbereich der Lebensmittelchemie.

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