Biological tissues and components, and synthetic substrates for conjunctival cell transplantation

Makuloluwa, Aruni Kumari; Hamill, Kevin J.; Rauz, Saaeha; Bosworth, Lucy A.; Haneef, Atikah; Romano, Vito; Williams, Rachel; Dartt, Darlene A.; Kaye, Stephen B.

doi:10.1016/j.jtos.2021.06.003

Cited by 10 publications

(4 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although goblet cells account for only 5%–10% of cells of the conjunctival epithelium, they play an irreplaceable role in lubrication, immunomodulation, and ocular surface homeostasis 31 . Conjunctival stem cells are able to differentiate into goblet cells and conjunctival epithelial cells under physiological conditions, but this process often fails to reappear in tissue‐engineered conjunctival equivalents 30,163 . For example, Witt et al preexpanded human conjunctival epithelial cells on porcine decellularized conjunctiva for 14 days in vitro, and a stratified epithelium with an average thickness of 15.15 μm was formed, but no goblet cells were found in the epithelium 17 .…”

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

“… 31 Conjunctival stem cells are able to differentiate into goblet cells and conjunctival epithelial cells under physiological conditions, but this process often fails to reappear in tissue‐engineered conjunctival equivalents. 30 , 163 For example, Witt et al preexpanded human conjunctival epithelial cells on porcine decellularized conjunctiva for 14 days in vitro, and a stratified epithelium with an average thickness of 15.15 μm was formed, but no goblet cells were found in the epithelium. 17 Methods to increase goblet cell numbers have mainly focused on directing resident stem cells toward goblet cell differentiation.…”

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

See 1 more Smart Citation

Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?

Yan

et al. 2023

Bioengineering & Transla Med

View full text Add to dashboard Cite

Reconstruction of posterior lamellar eyelids remains challenging due to their delicate structure, highly specialized function, and cosmetic concerns. Current clinically available techniques for posterior lamellar reconstruction mainly focus on reconstructing the contour of the eyelids. However, the posterior lamella not only provides structural support for the eyelid but also offers a smooth mucosal surface to facilitate globe movement and secrete lipids to maintain ocular surface homeostasis. Bioengineered posterior lamellar substitutes developed via acellular or cellular approaches have shown promise as alternatives to current therapies and encouraging outcomes in animal studies and clinical conditions. Here, we provide a brief reference on the current application of autografts, biomaterials, and tissue‐engineered substitutes for posterior lamellar eyelid reconstruction. We also shed light on future challenges and directions for eyelid regeneration strategies and offer perspectives on transitioning replacement strategies to regeneration strategies for eyelid reconstruction in the future.

show abstract

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?

Yan

et al. 2023

Bioengineering & Transla Med

View full text Add to dashboard Cite

show abstract

“…Understanding these factors is imperative to develop new therapeutic strategies to target mucin deficiency and regain ocular surface homeostasis upon pathology. Furthermore, the cultivation of goblet cells could open new therapeutic strategies to restore mucin-deficient, damaged conjunctival tissue in severe ocular surface disorders [10,19,30]. In this study, we investigated the functionality of the goblet cells more thoroughly in ex vivo conjunctival cultures to challenge the standard practice of characterizing goblet cells using only staining procedures.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures

Acker

Bogerd

Acker

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

One key element to the health of the ocular surface encompasses the presence of gel-forming mucins in the pre-ocular tear film. Conjunctival goblet cells are specialized epithelial cells that secrete mucins necessary for tear film stability and general homeostasis. Their dysfunction can be linked to a range of ocular surface inflammation disorders and chronic injuries. To obtain new perspectives and angles to tackle mucin deficiency, the need for an accurate evaluation of their presence and corresponding mucin secretion in ex vivo conjunctival cultures has become a requisite. In vitro, goblet cells show a significant decrease in the production and secretion of gel-forming mucins, accompanied by signs of dedifferentiation or transdifferentiation. Explant cultures on laminin-treated CLP-PEG hydrogels can, however, support the production of gel-forming mucins. Together, we challenge the current paradigm to evaluate the presence of cultured goblet cells solely based on their general mucin (MUC) content through imaging analyses, showing the need for additional techniques to assess the functionality of goblet cells. In addition, we broadened the gel-forming mucin profile of in vivo goblet cells with MUC5B and MUC6, while MUC2 and MUC6 is added to the profile of cultured goblet cells.

show abstract

“…Commonly used graft tissues such as human amniotic membrane or autologous mucous membrane have certain drawbacks, including limited availability, time-consuming preparation, and inconsistent tissue properties such as thickness or growth factor content, which have led to an increased focus on alternative tissues and materials [ 3 , 4 , 5 , 6 , 7 , 8 ]. In this context, extracellular matrix-based biomaterials and decellularized tissues are becoming more important and have already been used clinically [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Storage Conditions on Decellularized Porcine Conjunctiva

et al. 2023

View full text Add to dashboard Cite

Porcine decellularized conjunctiva (PDC) represents a promising alternative source for conjunctival reconstruction. Methods of its re-epithelialization in vitro with primary human conjunctival epithelial cells (HCEC) have already been established. However, a long-term storage method is required for a simplified clinical use of PDC. This study investigates the influence of several storage variants on PDC. PDC were stored in (1) phosphate-buffered saline solution (PBS) at 4 °C, (2) in glycerol-containing epithelial cell medium (EM/gly) at −80 °C and (3) in dimethyl sulfoxide-containing epithelial cell medium (EM/DMSO) at −196 °C in liquid nitrogen for two and six months, respectively. Fresh PDC served as control. Histological structure, biomechanical parameters, the content of collagen and elastin and the potential of re-epithelialization with primary HCEC under cultivation for 14 days were compared (n = 4–10). In all groups, PDC showed a well-preserved extracellular matrix without structural disruptions and with comparable fiber density (p ≥ 0.74). Collagen and elastin content were not significantly different between the groups (p ≥ 0.18; p ≥ 0.13, respectively). With the exception of the significantly reduced tensile strength of PDC after storage at −196 °C in EM/DMSO for six months (0.46 ± 0.21 MPa, p = 0.02), no differences were seen regarding the elastic modulus, tensile strength and extensibility compared to control (0.87 ± 0.25 MPa; p ≥ 0.06). The mean values of the epithelialized PDC surface ranged from 51.9 ± 8.8% (−196 °C) to 78.3 ± 4.4% (−80 °C) and did not differ significantly (p ≥ 0.35). In conclusion, all examined storage methods were suitable for storing PDC for at least six months. All PDC were able to re-epithelialize, which rules out cytotoxic influences of the storage conditions and suggests preserved biocompatibility for in vivo application.

show abstract

Biological tissues and components, and synthetic substrates for conjunctival cell transplantation

Cited by 10 publications

References 93 publications

Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?

Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?

Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures

Influence of Storage Conditions on Decellularized Porcine Conjunctiva

Contact Info

Product

Resources

About