Biological Valorization of Poly(ethylene terephthalate) Monomers for Upcycling Waste PET

Kim, Hee Taek; Kim, Jae Gyoon; Gil, Hyun; Kang, Myung Jong; Lee, Hyun Sook; Khang, Tae Uk; Yun, Eun Ju; Lee, Dong Hoon; Song, Bong Keun; Park, Si Jae; Joo, Jeong Chan; Kim, Kyoung Heon

doi:10.1021/acssuschemeng.9b03908

Cited by 184 publications

(127 citation statements)

References 59 publications

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“…Hence, various bio-based production methods using renewable feedstocks have recently been developed [1,2]. To address the growing demand for the production of value-added products, such as platform chemicals and biopolymers from renewable resources, several engineered microorganisms have been developed [3][4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Effect of DR1558, a Deinococcus radiodurans response regulator, on the production of GABA in the recombinant Escherichia coli under low pH conditions

et al. 2020

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Background: Gamma aminobutyric acid (GABA) is an important platform chemical, which has been used as a food additive and drug. Additionally, GABA is a precursor of 2-pyrrolidone, which is used in nylon synthesis. GABA is usually synthesized from glutamate in a reaction catalyzed by glutamate decarboxylase (GAD). Currently, there are several reports on GABA production from monosodium glutamate (MSG) or glucose using engineered microbes. However, the optimal pH for GAD activity is 4, which is the limiting factor for the efficient microbial fermentative production of GABA as fermentations are performed at pH 7. Recently, DR1558, a response regulator in the two-component signal transduction system was identified in Deinococcus radiodurans. DR1558 is reported to confer cellular robustness to cells by binding the promoter regions of genes via DNA-binding domains or by binding to the effector molecules, which enable the microorganisms to survive in various environmental stress conditions, such as oxidative stress, high osmotic shock, and low pH. Results: In this study, the effect of DR1558 in enhancing GABA production was examined using two different strategies: whole-cell bioconversion of GABA from MSG and direct fermentative production of GABA from glucose under acidic culture conditions. In the whole-cell bioconversion, GABA produced by E. coli expressing GadBC and DR1558 (6.52 g/L GABA from 13 g/L MSG•H 2 O) in shake flask culture at pH 4.5 was 2.2-fold higher than that by E. coli expressing only GadBC (2.97 g/L of GABA from 13 g/L MSG•H 2 O). In direct fermentative production of GABA from glucose, E. coli ∆gabT expressing isocitrate dehydrogenase (IcdA), glutamate dehydrogenase (GdhA), GadBC, and DR1558 produced 1.7-fold higher GABA (2.8 g/L of GABA from 30 g/L glucose) than E. coli ∆gabT expressing IcdA, GdhA, and GadBC (1.6 g/L of GABA from 30 g/L glucose) in shake flask culture at an initial pH 7.0. The transcriptional analysis of E. coli revealed that DR1558 conferred acid resistance to E. coli during GABA production. The fed-batch fermentation of E. coli expressing IcdA, GdhA, GadBC, and DR1558 performed at pH 5.0 resulted in the final GABA titer of 6.16 g/L by consuming 116.82 g/L of glucose in 38 h. Conclusion: This is the first report to demonstrate GABA production by acidic fermentation and to provide an engineering strategy for conferring acid resistance to the recombinant E. coli for GABA production.

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Section: Introductionmentioning

confidence: 99%

Effect of DR1558, a Deinococcus radiodurans response regulator, on the production of GABA in the recombinant Escherichia coli under low pH conditions

et al. 2020

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“…In addition, the product of glycolic acid obtained from EG–fermenting process of microbe. DCD 1,2 DCD, 1,2-di hydroxy-3,5-cyclo hexa diene-1,4-di carboxylate [ 148 ].…”

Section: The Recent Development Of Pet Chemical Recyclingmentioning

confidence: 99%

Strategic Possibility Routes of Recycled PET

2021

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The polyethylene terephthalate (PET) application has many challenges and potential due to its sustainability. The conventional PET degradation was developed for several technologies to get higher yield products of ethylene glycol, bis(2-hydroxyethyl terephthalate) and terephthalic acid. The chemical recycling of PET is reviewed, such as pyrolysis, hydrolysis, methanolysis, glycolysis, ionic-liquid, phase-transfer catalysis and combination of glycolysis–hydrolysis, glycolysis–methanolysis and methanolysis–hydrolysis. Furthermore, the reaction kinetics and reaction conditions were investigated both theoretically and experimentally. The recycling of PET is to solve environmental problems and find another source of raw material for petrochemical products and energy.

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“…SYK-6. Another study on upcycling PET degradation monomers describes directing TPA toward the production of high-value aromatics PCA, gallic acid (GA), pyrogallol (PG), catechol (CA), muconic acid (MA), vanillic acid (VA), and EG toward glycolic acid (GLA) (Kim et al, 2019). They engineered E. coli strains to harbor relevant metabolic pathways to funnel TPA into the desired product using single or combined reactions of hydroxylation, decarboxylation, oxidative ring cleavage, and methylation.…”

Section: Engineering Whole-cell Biocatalysts To Upcycle Plasticmentioning

confidence: 99%

Engineering Microbes to Bio-Upcycle Polyethylene Terephthalate

Dissanayake

Jayakody

2021

Front. Bioeng. Biotechnol.

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Polyethylene terephthalate (PET) is globally the largest produced aromatic polyester with an annual production exceeding 50 million metric tons. PET can be mechanically and chemically recycled; however, the extra costs in chemical recycling are not justified when converting PET back to the original polymer, which leads to less than 30% of PET produced annually to be recycled. Hence, waste PET massively contributes to plastic pollution and damaging the terrestrial and aquatic ecosystems. The global energy and environmental concerns with PET highlight a clear need for technologies in PET “upcycling,” the creation of higher-value products from reclaimed PET. Several microbes that degrade PET and corresponding PET hydrolase enzymes have been successfully identified. The characterization and engineering of these enzymes to selectively depolymerize PET into original monomers such as terephthalic acid and ethylene glycol have been successful. Synthetic microbiology and metabolic engineering approaches enable the development of efficient microbial cell factories to convert PET-derived monomers into value-added products. In this mini-review, we present the recent progress of engineering microbes to produce higher-value chemical building blocks from waste PET using a wholly biological and a hybrid chemocatalytic–biological strategy. We also highlight the potent metabolic pathways to bio-upcycle PET into high-value biotransformed molecules. The new synthetic microbes will help establish the circular materials economy, alleviate the adverse energy and environmental impacts of PET, and provide market incentives for PET reclamation.

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Biological Valorization of Poly(ethylene terephthalate) Monomers for Upcycling Waste PET

Cited by 184 publications

References 59 publications

Effect of DR1558, a Deinococcus radiodurans response regulator, on the production of GABA in the recombinant Escherichia coli under low pH conditions

Effect of DR1558, a Deinococcus radiodurans response regulator, on the production of GABA in the recombinant Escherichia coli under low pH conditions

Strategic Possibility Routes of Recycled PET

Engineering Microbes to Bio-Upcycle Polyethylene Terephthalate

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