Biologically Active Polypeptides in Mouse Submandibular Gland

Tsukitani

et al. 1983

mentioning

confidence: 99%

Use of lectins for differential localization of secretory materials of granular convoluted tubules and ducts in the submandibular gland.

Naito

Tsukitani

et al. 1983

“…GCT cells in the salivary glands are unique cells which markedly exist in the SMG in mature male mice, and secretory granules are the main source of salivary gland growth factors such as EGF and NGF (1,8,12). GCT cells in rodent SMGs were found in mice, rats and hamsters, and EGF also exists in the rat GCT cells, however, the biological roles of hamster GCT cells are not yet understood.…”

Section: Discussionmentioning

confidence: 99%

“…In GCT cells in murine SMGs (mouse, rat, and hamster) particular structures exist for biosynthesis and storage of salivary gland growth factors (EGF and NGF), renin, kallikrein and esteroprotease (1,7,8,12,18). The preceding papers have reported that ductal segments of hamster SMGs were crossreacted with mouse EGF (11) and slightly positive to keratin proteins from immunohistochemical method (20).…”

Section: Immunocytochemicalmentioning

confidence: 99%

Vimentin characteristically exists in granular convoluted tubules of hamster submandibular glands. Immunohistochemical studies on comparative localization of filament proteins in salivary gland ducts.

Mori

Hosaka

et al. 1985

Immunocytochemicaldetection of vimentin class of filament proteins except for keratin is reported in ductal segments of rodent submandibular glands (SMGs) and compared to stainabilities of different ductal systems; intercalated duct (ICD), granular convoluted tubule (GCT), striated duct (SD) and excretory duct (ED), and in the major salivary glands; parotid (PG), submandibular (SMG) and sublingual glands (SLG). Filament proteins; vimentin, desmin, filamin, actin and myosin were examined by immunocytochemical technique. Vimentin characteristically was confined to the GCT cells of hamster SMGs, and was distributed in luminal surface of the SD cells of guinea pigs, where it was negative in the SMGs of rats and mice, but positive in SLG duct cells. Vimentin was also positive in the ICD cells of hamster SLG.Desmin was slightly positive in the duct cells of hamsters, and intensely positive in luminal side of the SD cells of SMG and PG in guinea pigs, and of SLG in rats. Trace amounts of desmin were also noted in the GCT cells of mice and rats. Filamin was strongly positive in smooth muscles of blood vessels and trace or slightly positive in ductal cells from all the specimens.Actin was also strongly evident in smooth muscles, and slightly reactive to hamsters GCT cells, guinea pigs, and rats ductal cells and was negative in mice. Myosin was slightly positive in the GCT cells of hamsters and in the SD cells of guinea pig.

show abstract

“…The GCT cells of SMGs contain biologically active peptides like EGF and NGF (1,5,8,9,12), lectin positive materials (11,15,16), and protein bound SH and SS groups (18) (19). However, the present results indicate that the GCT cells of mice and rats are slightly positive for keratin proteins when Bouin's, Helly's, alcohol and/or acetone fixative is used, but almost or completely negative in the case of Carnoy's, formalin, Zamboni's, Orth's and PLP fixatives.…”

Section: Discussionmentioning

confidence: 99%

“…Salivary glands of mammals are composed of acinar compartments and ductal segments which are classified into intercalated duct (ICD), striated duct (SD) and excretory duct (ED). Granular convoluted tubule (GCT) cells which include biological polypeptides like EGF and NGF, also exist as special structural components in SMGs of mice and rats (1,5,8,9,12).…”

mentioning

confidence: 99%

Comparison of effectiveness of different fixatives for keratin distribution in ductal segments of salivary glands.

Sumitomo

Noda

et al. 1985