Vimentin characteristically exists in granular convoluted tubules of hamster submandibular glands. Immunohistochemical studies on comparative localization of filament proteins in salivary gland ducts.

Abstract

Immunocytochemicaldetection of vimentin class of filament proteins except for keratin is reported in ductal segments of rodent submandibular glands (SMGs) and compared to stainabilities of different ductal systems; intercalated duct (ICD), granular convoluted tubule (GCT), striated duct (SD) and excretory duct (ED), and in the major salivary glands; parotid (PG), submandibular (SMG) and sublingual glands (SLG). Filament proteins; vimentin, desmin, filamin, actin and myosin were examined by immunocytochemical technique. Vimentin characteristically was confined to the GCT cells of hamster SMGs, and was distributed in luminal surface of the SD cells of guinea pigs, where it was negative in the SMGs of rats and mice, but positive in SLG duct cells. Vimentin was also positive in the ICD cells of hamster SLG.Desmin was slightly positive in the duct cells of hamsters, and intensely positive in luminal side of the SD cells of SMG and PG in guinea pigs, and of SLG in rats. Trace amounts of desmin were also noted in the GCT cells of mice and rats. Filamin was strongly positive in smooth muscles of blood vessels and trace or slightly positive in ductal cells from all the specimens.Actin was also strongly evident in smooth muscles, and slightly reactive to hamsters GCT cells, guinea pigs, and rats ductal cells and was negative in mice. Myosin was slightly positive in the GCT cells of hamsters and in the SD cells of guinea pig.

An Immunohistochemical Study of Cytokeratin and Vimentin in Benign and Malignant Thyroid Lesions

“…Therefore, it was considered that papillary carcinoma displayed increased co-expression of CK and vimentin antigens in the cytoplasm, suggesting the possibility that a quantitative or qualitative alteration of both antigens might occur in papillary carcinoma. It is generally believed that keratin is specific for epithelial cells, whereas vimentin is specific for mesoderm-derived cells (14-16), although some exceptions to this concept have been reported (6,7,(17)(18)(19)(20)(21)(22). Co-expression of CK and vimentin has been reported in metastatic epithelial tumor cells present in body fluid (20) and in cultured epithelial cells (21,22), which adapt to their new growth condition by development of a vimentin cytoskeleton.…”

Interlobular excretory ducts of mammalian salivary glands: Structural and histochemical review

“…It is not known at present if these inclusions are in some way correlated with either the ecology or behavior of this fossorial species. Crystalloids are present in the taller ED cells in the sublingual gland of the African multimammate rodent, Praomys natalensis (Toyoshima and Tandler, 1991), and in the Caselitz et al, 1981aCaselitz et al, , 1981bCaselitz et al, , 1986Burns et al, 1988;Dardick et al, 1988SG Mori et al, 1985bHosaka et al, 1985;Born et al, 1987;Geiger et al, 1987;Takai et al, 1988;Gustafsson et al, 1988;Lee et al, 1990;Mori, 1991;Hamakawa et al, 1999SM Nakai et al, 1985Leitner, 1987 Golden hamster SG Takai et al, 1985a;Mori et al, 1985a;Mori, 1991 EDs of the accessory submandibular glands of the longwinged bat, Miniopterus magnater (Tandler et al, 1997a). EDs in the parotid glands of seven species of fruit bats in the genus Artibeus resemble SDs in the same gland, but occasionally include one or several dark cells in a given sectioned duct (Fig.…”