Biologically Programmed Nanoparticle Assembly

Mann, Stephen; Shenton, Wayne; Li, M.; Connolly, Stephen; Fitzmaurice, Donald

doi:10.1002/(sici)1521-4095(200001)12:2<147::aid-adma147>3.0.co;2-u

Cited by 337 publications

(251 citation statements)

References 5 publications

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“…Gold nanoparticles (GNPs) with a size ranging from several to hundreds of nanometers show a bright pinkish color due to plasmon resonance; they have been used in stained glasses for several hundred years. A simple means of anchoring different ligand molecules onto particle surfaces allows their utilization as colloidal sensors [16][17][18]. In particular, color changes induced by the aggregation of nanometer-sized gold particles provide a basis for a simple yet highly selective method for detecting specific biological events between anchored ligand molecules and receptor molecules in the milieu.…”

Section: Introductionmentioning

confidence: 99%

Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Nagasaki

2010

Science and Technology of Advanced Materials

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A poly(ethylene glycol)-b-poly [2-(N,N-dimethylamino)ethyl methacrylate] block copolymer possessing a reactive acetal group at the end of the poly(ethylene glycol) (PEG) chain, that is, acetal-PEG-b-PAMA, was synthesized by a proprietary polymerization technique. Gold nanoparticles (GNPs) were prepared using the thus-synthesized acetal-PEG-b-PAMA block copolymer. The PEG-b-PAMA not only acted as a reducing agent of aurate ions but also attached to the nanoparticle surface. The GNPs obtained had controlled sizes and narrow size distributions. They also showed high dispersion stability owing to the presence of PEG tethering chains on the surface. The same strategy should also be applicable to the fabrication of semiconductor quantum dots and inorganic porous nanoparticles. The preparation of nanoparticles in situ, i.e. in the presence of acetal-PEG-b-PAMA, gave the most densely packed polymer layer on the nanoparticle surface; this was not observed when coating preformed nanoparticles. PEG/polyamine block copolymer was more functional on the metal surface than PEG/polyamine graft copolymer, as confirmed by angle-dependent x-ray photoelectron spectroscopy. We successfully solubilized the C 60 fullerene into aqueous media using acetal-PEG-b-PAMA. A C 60 /acetal-PEG-b-PAMA complex with a size below 5 nm was obtained by dialysis. The preparation and characterization of these materials are described in this review.

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Section: Introductionmentioning

confidence: 99%

Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Nagasaki

2010

Science and Technology of Advanced Materials

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“…17 The biotinPEG(2000)-PE-coated NPs are functionalized with biotin at the end of each PEG chain. These NPs were used for site-specific binding to avidin-patterned substrates.…”

Section: Resultsmentioning

confidence: 99%

Bio-functionalization of Monodisperse Magnetic Nanoparticles and Their Use as Biomolecular Labels in a Magnetic Tunnel Junction Based Sensor

et al. 2005

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Monodisperse magnetic nanoparticles (NPs) could enable the ultra-sensitive magnetic detection of biological analytes. However, rendering these particles biocompatible has remained a challenge. We report the biofunctionalization and detection of 12-nm manganese ferrite NPs. We have achieved the site-specific binding of biotin-functionalized NPs onto avidin-patterned silicon oxide substrates and DNA-functionalized NPs onto complementary DNA-patterned silicon oxide substrates. Utilizing scanning SQUID microscopy, we show that these substrate-bound NPs retain their magnetic properties. Finally, we demonstrate a novel method of detecting either protein binding or DNA hybridization at room temperature using the NPs and a magnetic tunnel-junction-based biosensor situated in orthogonal magnetic fields.

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“…This recognition attribute, found in for example receptors and antibodies, can also be used to trigger nanoparticle assembly. [28][29] Proteins are typically not very robust and may irreversibly lose their native structure and hence functionality because of changes in their physicochemical environment, such as increasing temperature, drying, high or low pH, or in the presence of a solid substrate.…”

Section: Nanotechnology and Bionanotechnologymentioning

confidence: 99%

Polypeptide functionalized gold nanoparticles for bioanalytical applications

Selegård¹

2014

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Detection strategies that allow for simple, rapid, cost efficient and sensitive monitoring of proteins and their interactions with biomolecules are of great importance in drug development and diagnostics. This thesis describes the development of bioanalytical applications based on the tunable self-assembly of gold nanoparticles functionalized with a de novo designed polypeptide. Strategies for protein affinity sensing and for detection of several fundamentally important biological processes have been investigated, including Zn 2+ -mediated coordination between polypeptides and low molecular weight chelants and protease and phosphatase activity.A Zn 2+ responsive synthetic polypeptide designed to fold into a helix-loop-helix motif and dimerize into a four-helix bundle has been used to control the stability and self-assembly of gold nanoparticles. This polypeptide has a high negative net charge at neutral pH as a consequence of its many glutamic acid residues, efficiently preventing folding and dimerization due to charge repulsion. Zn 2+ coordination provides a means to trigger folding and dimerization at neutral pH. The polypeptide can be readily attached to gold nanoparticles via a cysteine residue in the loop region, retaining its folding properties and responsiveness to Zn 2+ . The polypeptide functionalized gold nanoparticles display excellent colloidal stability but aggregate reversibly after addition of millimolar concentrations of Zn 2+ . Aggregates are dense with a defined interparticle distance corresponding to the size of the four-helix bundle, resulting in a distinct red shift of the localized surface plasmon resonance band.Three completely different strategies for colorimetric biosensing have been developed, all being based on the same responsive hybrid nanomaterial. In the first strategy a synthetic receptor was co-immobilized on the gold nanoparticles together with the Zn 2+ responsive polypeptide. Protein analyte binding to the receptor could be detected as this interaction sterically prevented aggregation induced by Zn 2+ . In the second strategy the reduction in colloidal stability caused by specific proteolytic cleavage of the immobilized polypeptide was exploited to monitor the enzymatic activity. The third strategy utilized the sensitivity of the system to small variations in Zn 2+ concentration. The presence of low molecular weight chelants was found to influence the mode of aggregation, both by sequestering Zn 2+ and through the formation of ternary complexes involving the polypeptides, which prevented dimerization and thus aggregation. This approach was further developed into a generic concept for phosphatase detection exploiting the different affinity of enzyme substrates and reaction products for Zn 2+ . VThe flexibility of the different detection schemes enables detection of a large number of analytes by exploiting the tunable stability of the nanoparticles and the possibilities to effectively decouple the recognition event and the nanoparticle stability modulation. VI POPULÄRVET...

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Biologically Programmed Nanoparticle Assembly

Cited by 337 publications

References 5 publications

Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Bio-functionalization of Monodisperse Magnetic Nanoparticles and Their Use as Biomolecular Labels in a Magnetic Tunnel Junction Based Sensor

Polypeptide functionalized gold nanoparticles for bioanalytical applications

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