Biologically stable threose nucleic acid-based probes for real-time microRNA detection and imaging in living cells

Wang, Fei; Liu, Ling Sum; Li, Pan; Leung, Hoi Man; Tam, Dick Yan; Lo, Pik Kwan

doi:10.1016/j.omtn.2021.12.040

Cited by 10 publications

(6 citation statements)

References 51 publications

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“…TNA can participate in antiparallel duplex formation and cross-pairing with DNA and RNA in a sequence-specific manner following the Watson–Crick base pairing rule. 40 The characteristics like stability in the physiological environment, high specificity and affinity toward DNA/RNA sequences, nuclease resistance, and effective cellular uptake may make TNA an important candidate for the development of robust XNA-based biosensors in the near future. 40 The appeal of the arabinose-modified oligonucleotides, i.e., ANA and F-ANA, is based on their resistance to nuclease enzymes and their capacity to bind to target RNA.…”

Section: The Xnas As Troubleshootermentioning

confidence: 99%

“… 40 The characteristics like stability in the physiological environment, high specificity and affinity toward DNA/RNA sequences, nuclease resistance, and effective cellular uptake may make TNA an important candidate for the development of robust XNA-based biosensors in the near future. 40 The appeal of the arabinose-modified oligonucleotides, i.e., ANA and F-ANA, is based on their resistance to nuclease enzymes and their capacity to bind to target RNA. Their binding affinities for RNA are drastically different as ANA binds to RNA with comparatively low affinity, whereas F-ANA forms thermally stable duplexes with RNA.…”

Section: The Xnas As Troubleshootermentioning

confidence: 99%

“…Another interesting XNA is TNA, which is completely resistant to digestion by nuclease enzyme. TNA can participate in antiparallel duplex formation and cross-pairing with DNA and RNA in a sequence-specific manner following the Watson–Crick base pairing rule . The characteristics like stability in the physiological environment, high specificity and affinity toward DNA/RNA sequences, nuclease resistance, and effective cellular uptake may make TNA an important candidate for the development of robust XNA-based biosensors in the near future .…”

Section: The Xnas As Troubleshootermentioning

confidence: 99%

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XNAs: A Troubleshooter for Nucleic Acid Sensing

2022

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The strategies for nucleic acid sensing based on nucleic acid hybridization between the target sequence and the capture probe sequence are considered to be largely successful as far as detection of a specific target of known sequence is concerned. However, when compared with other complementary methods, like direct sequencing, a number of results are still found to be either “false positives” or “false negatives”. This suggests that modifications in these strategies are necessary to make them more accurate. In this minireview, we propose that one way toward improvement could be replacement of the DNA capture probes with the xeno nucleic acid or XNA capture probes. This is because the XNAs, especially the locked nucleic acid, the peptide nucleic acid, and the morpholino, have shown better single nucleobase mismatch discrimination capacity than the DNA capture probes, indicating their capacity for more precise detection of nucleic acid sequences, which is beneficial for detection of gene stretches having point mutations. Keeping the current trend in mind, this minireview will include the recent developments in nanoscale, fluorescent label-free applications, and present the cases where the XNA probes show clear advantages over the DNA probes.

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Section: The Xnas As Troubleshootermentioning

confidence: 99%

Section: The Xnas As Troubleshootermentioning

confidence: 99%

Section: The Xnas As Troubleshootermentioning

confidence: 99%

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XNAs: A Troubleshooter for Nucleic Acid Sensing

2022

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“…Aptamers based on the genetic polymer of threose nucleic acid (TNA), for example, remain functional in the presence of nucleases that rapidly degrade their natural DNA and RNA counterparts . This property, along with a high safety profile, − provides compelling evidence for TNA as a candidate genetic material for diagnostic and therapeutic applications that demand high biological stability.…”

Section: Introductionmentioning

confidence: 99%

Highly Parallelized Screening of Functionally Enhanced XNA Aptamers in Uniform Hydrogel Particles

et al. 2023

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Xeno-nucleic acid (XNA) aptamers based on evolvable nonnatural genetic polymers hold enormous potential as future diagnostic and therapeutic agents. However, time-consuming and costly procedures requiring the purification of individual XNA sequences produced by largescale polymerase-mediated primer extension reactions pose a major bottleneck to the discovery of highly active XNA motifs for biomedical applications. Here, we describe a straightforward approach for rapidly surveying the binding properties of XNA aptamers identified by in vitro selection. Our strategy involves preparing XNA aptamer particles in which many copies of the same aptamer sequence are distributed throughout the gel matrix of a polyacrylamide-encapsulated magnetic particle. Aptamer particles are then screened by flow cytometry to assess target binding affinity and deduce structure−activity relationships. This generalizable and highly parallel assay dramatically accelerates the pace of secondary screening by allowing a single researcher to evaluate 48−96 sequences per day.

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“…Functional nucleic acids(FNAs) are the kind of nucleic acids that have specific functions beyond traditional genetic roles [4,5] . In the past three decades, scientists have made great efforts on FNAs, and have a deep understanding of their molecular structures and biological functions [6] . FNAs, such as aptamers, nucleic acid enzymes(NAzymes) and molecular beacons(MBs) have been widely applied in screening, sensing, and imaging fields [7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Functional Xeno Nucleic Acids for Biomedical Application

Huan

et al. 2022

Chem. Res. Chin. Univ.

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Functional nucleic acids(FNAs) refer to a type of oligonucleotides with functions over the traditional genetic roles of nucleic acids, which have been widely applied in screening, sensing and imaging fields. However, the potential application of FNAs in biomedical field is still restricted by the unsatisfactory stability, biocompatibility, biodistribution and immunity of natural nucleic acids(DNA/RNA). Xeno nucleic acids(XNAs) are a kind of nucleic acid analogues with chemically modified sugar groups that possess improved biological properties, including improved biological stability, increased binding affinity, reduced immune responses, and enhanced cell penetration or tissue specificity. In the last two decades, scientists have made great progress in the research of functional xeno nucleic acids, which makes it an emerging attractive biomedical application material. In this review, we summarized the design of functional xeno nucleic acids and their applications in the biomedical field.

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Biologically stable threose nucleic acid-based probes for real-time microRNA detection and imaging in living cells

Cited by 10 publications

References 51 publications

XNAs: A Troubleshooter for Nucleic Acid Sensing

XNAs: A Troubleshooter for Nucleic Acid Sensing

Highly Parallelized Screening of Functionally Enhanced XNA Aptamers in Uniform Hydrogel Particles

Functional Xeno Nucleic Acids for Biomedical Application

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