Biologically synthesized crude calcium lactate as a substrate for propylene glycol production

Binczarski, Michał; Berłowska, Joanna; Stanishevsky, Andrei; Witońska, Izabela

doi:10.1039/c6ra20722g

Cited by 16 publications

(19 citation statements)

References 36 publications

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“…According to James [ 32 ] amino acids adsorb in Pd centers. Our studies on sugar beet pulp hydrolysates showed the presence of Ala, Pro in the concentrations over 110 mmol/L; Leu, Tyr over 80 mmol/L and Asp, Glu, Gly, His, Arg, Val, Cys, Phe over 20 mmol/L [ 33 ]. Blocking the centers on the Pd surface could hinder the reduction of FA to THFA, which might explain the higher selectivity to FA observed for distillates obtained from beet leaves.…”

Section: Resultsmentioning

confidence: 99%

Concept for Recycling Waste Biomass from the Sugar Industry for Chemical and Biotechnological Purposes

et al. 2017

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The objective of this study was to develop a method for the thermally-assisted acidic hydrolysis of waste biomass from the sugar industry (sugar beet pulp and leaves) for chemical and biotechnological purposes. The distillates, containing furfural, can be catalytically reduced directly into furfurayl alcohol or tetrahydrofurfuryl alcohol. The sugars present in the hydrolysates can be converted by lactic bacteria into lactic acid, which, by catalytic reduction, leads to propylene glycol. The sugars may also be utilized by microorganisms in the process of cell proliferation, and the biomass obtained used as a protein supplement in animal feed. Our study also considered the effects of the mode and length of preservation (fresh, ensilage, and drying) on the yields of furfural and monosaccharides. The yield of furfural in the distillates was measured using gas chromatography with flame ionization detector (GC-FID). The content of monosaccharides in the hydrolysates was measured spectrophotometrically using enzymatic kits. Biomass preserved under all tested conditions produced high yields of furfural, comparable to those for fresh material. Long-term storage of ensiled waste biomass did not result in loss of furfural productivity. However, there were significant reductions in the amounts of monosaccharides in the hydrolysates.

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Section: Resultsmentioning

confidence: 99%

Concept for Recycling Waste Biomass from the Sugar Industry for Chemical and Biotechnological Purposes

et al. 2017

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“…However, it is increasingly converted into bioproducts such as yeast biomass (6) or biogas (2). This study, conducted at Lodz University of Technology, shows for the first time that SBP is also a promising feedstock for lactic acid biosynthesis (7,8).…”

Section: Introductionmentioning

confidence: 99%

“…However, some disadvantages may occur during scaling-up. The separate hydrolysis of sugar beet pulp was successfully implemented in a 3 m 3 stirred reactor at the Sugar factory in Dobrzelin (7,42,43), but with smaller enzyme doses such parameters as the presence of a dispersed gas phase and turbulent flow may be more significant for the efficiency of the process.…”

Section: Separate Hydrolysis and Fermentation (Shf)mentioning

confidence: 99%

Enzymatic Conversion of Sugar Beet Pulp: A Comparison of Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation for Lactic Acid Production

Berłowska

Cieciura-Włoch

Kalinowska

et al. 2018

Food Technol. Biotechnol.

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SummaryThis study compares the efficiency of lactic acid production by separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF) of sugar beet pulp (SBP), a by-product of industrial sugar production. In experiments, SBP was hydrolyzed using 5 commercial enzymes. A series of shake flask scale fermentations and was conducted using 5 selected strains of lactic acid bacteria (LAB). The differences in the activities of the enzymes for degrading the principal SBP components were reflected in the different yields of total reducing sugars. The highest yields from hydrolysis and the lowest quantities of insoluble residues were obtained using a mixture (1:1) of Viscozyme and Ultraflo Max. In the SHF process, only a portion of the soluble sugars released by the enzymes from the SBP was assimilated by the LAB strains. In SSF, low enzyme loads revealed reductions in the efficiency of sugar accumulation. The risk of carbon catabolic repression was reduced. Our results suggest www.ftb.com.hrPlease note that this is an unedited version of the manuscript that has been accepted for publication. This version will undergo copyediting and typesetting before its final form for publication. We are providing this version as a service to our readers. The published version will differ from this one as a result of linguistic and technical corrections and layout editing. that SSF has advantages over SHF, including lower processing costs and higher productivity.Lactic acid yield in SSF mode (around 30 g/L) was 80-90 % higher than that for SHF.

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“…The biomass was saccharified in a 3 m 3 reactor for 16 h at 50 • C, using a mixture (1:1) of two multi-enzyme preparations made by Novozymes: Viscozyme ® (Bagsvaerd, Denmark) and Ultraflo ® Max (Bagsvaerd, Denmark) (0.03 L/kg of sugar beet pulp dry weight). Saccharification was stopped by heating (80 • C for 10 min) [44].…”

Section: Feedstock: Sugar Beet Pulp Hydrolyzatementioning

confidence: 99%

Integrated Bioethanol Fermentation/Anaerobic Digestion for Valorization of Sugar Beet Pulp

et al. 2017

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Large amounts of waste biomass are generated in sugar factories from the processing of sugar beets. After diffusion with hot water to draw the sugar from the beet pieces, a wet material remains called pulp. In this study, waste sugar beet pulp biomass was enzymatically depolymerized, and the obtained hydrolyzates were subjected to fermentation processes. Bioethanol, biomethane, and biohydrogen were produced directly from the substrate or in combined mode. Stillage, a distillery by-product, was used as a feedstock for anaerobic digestion. During biosynthesis of ethanol, most of the carbohydrates released from the sugar beet pulp were utilized by a co-culture of Saccharomyces cerevisiae Ethanol Red, and Scheffersomyces stipitis LOCK0047 giving 12.6 g/L of ethanol. Stillage containing unfermented sugars (mainly arabinose, galactose and raffinose) was found to be a good substrate for methane production (444 dm 3 CH 4 /kg volatile solids (VS)). Better results were achieved with this medium than with enzymatic saccharified biomass. Thermal pre-treatment and adjusting the pH of the inoculum resulted in higher hydrogen production. The largest (p < 0.05) hydrogen yield (252 dm 3 H 2 /kg VS) was achieved with sugar beet stillage (SBS). In contrast, without pre-treatment the same medium yielded 35 dm 3 H 2 /kg VS. However, dark fermentation of biohydrogen was more efficient when sugar beet pulp hydrolyzate was used.

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Biologically synthesized crude calcium lactate as a substrate for propylene glycol production

Abstract: Lactic acid (LA) can be obtained easily through fermentation of saccharides, and is an extremely useful building block for the synthesis of valuable chemicals, including propylene glycol (PG).

Cited by 16 publications

References 36 publications

Concept for Recycling Waste Biomass from the Sugar Industry for Chemical and Biotechnological Purposes

Concept for Recycling Waste Biomass from the Sugar Industry for Chemical and Biotechnological Purposes

Enzymatic Conversion of Sugar Beet Pulp: A Comparison of Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation for Lactic Acid Production

Integrated Bioethanol Fermentation/Anaerobic Digestion for Valorization of Sugar Beet Pulp

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