Biology and Control of Maize Chlorotic Mottle Virus

Uyemoto, J. K.

doi:10.1094/pd-67-7

Cited by 59 publications

(46 citation statements)

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“…According to Nelson et al (2011), most effective control involves an integration of cultural practices with insecticides and host resistance. Crop rotation for at least two seasons with alternative non-cereal crops has been reported to effectively control MCMV (Uyemoto, 1983). A great section of farmers (23.3%) reported using home saved seed for planting, which presents a big risk of MLN infection.…”

Section: Discussionmentioning

confidence: 99%

Status of maize lethal necrosis in eastern Uganda

Kagoda¹,

Gidoi²,

Isabirye³

2016

Afr. J. Agric. Res.

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Section: Discussionmentioning

confidence: 99%

Status of maize lethal necrosis in eastern Uganda

Kagoda¹,

Gidoi²,

Isabirye³

2016

Afr. J. Agric. Res.

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“…SCMV was reported several years ago in Kenya (Louie 1980) and South Africa (Handley et al 1998), but MCMV is new to Africa. MCMV was first identified in Peru in 1973 (Castillo and Hebert 1974) and subsequently reported in the USA, parts of Latin America, and China (Niblett and Claflin 1978;Uyemoto 1983;Xie et al 2011). MLN has also been reported in Rwanda (Adams et al 2014) and Democratic Republic of Congo (Lukanda et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…If a maize field is infected early in the cropping cycle, complete yield loss may occur (Uyemoto 1983;Wangai et al 2012). The USDA Foreign Agricultural Service estimates yield losses in Kenya as high as 10% for the 2014/15 marketing season amounting to over US$50 million (USDA 2014).…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of tropical maize inbred lines for resistance to maize lethal necrosis disease

et al. 2017

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Maize lethal necrosis (MLN) disease is a recent outbreak in eastern Africa and has emerged as a significant threat to maize production in the region. The disease is caused by the co-infection of Maize chlorotic mottle virus and any member of potyviridae family. A total of 28 maize inbred lines with varying levels of tolerance to MLN were crossed in a halfdiallel mating design, and the resulting 340 F 1 crosses and four commercial checks were evaluated under MLN artificial inoculation at Naivasha, Kenya in 2015 and 2016 using an alpha lattice design with two replications. The objectives of the study were to (i) investigate the magnitude of general combining ability variance (r GCA 2 ) and specific combining ability variance (r SCA 2 ) and their interaction with years; (ii) evaluate the efficiencies of GCA based prediction and hybrid performance by means of a cross-validation procedure; (iii) estimate trait correlations in the hybrids; and (iv) identify the MLN tolerant single cross hybrids to be used as female parents for threeway cross hybrids. Results of the combined analysis of variance revealed that both GCA and SCA effects were significant (P \ 0.05) for all traits except for ear rot. For MLN scores at early and late stages, GCA effects were 2.5-3.5 times higher than SCA effects indicating that additive gene action is more important than non-additive gene action. The GCA based prediction efficiency for MLN resistance and grain yield accounted for 67-90% of the variations in the hybrid performance suggesting that GCA-based prediction can be proposed to predict MLN resistance and grain yield prior to field evaluation. Three parents, CKDHL120918, CML550, and CKLTI0227 with significant GCA effects for GY (0.61-1.21; P \ 0.05) were the most resistant to MLN. Hybrids ''CKLTI0227 9 CML550'', ''CKDHL120918 9 CKLTI0138'', and ''CKDHL120918 9 CKLTI0136'' ranked among the best performing hybrids with grain yield of 6.0-6.6 t/ha compared with mean yield of commercial check hybrids (0.6 t/ha). The MLN tolerant inbred lines and single cross hybrids identified in this study could be used to improve MLN tolerance in both public and private sector maize breeding programs in eastern Africa.

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“…Typical symptoms of MCMV include mild mosaic, severe stunting, leaf necrosis, premature plant death, shortened male inflorescences with few spikes, and shortened, malformed, partially filled ears (Castillo and Hebert, 1974;Niblett and Clafin, 1978;Nault et al, 1981;Uyemoto et al, 1981). MCMV often induces corn lethal necrosis (CLN) resulting from synergistic interaction between this virus and maize dwarf mosaic virus (MDMV) (Niblett and Clafin, 1978;Goldberg and Brakke, 1987), wheat streak mosaic virus (WSMV) (Scheets, 1998), or sugarcane mosaic potyvirus (SCMV) (Uyemoto et al, 1980), leading to serious yield losses in corn (Uyemoto, 1983;Scheets, 1998;Morales et al, 1999;Xie et al, 2011). Adults of six species of chrysomelid beetle were reported to transmit MCMV under experimental conditions (Nault et al, 1978), and seed transmission was also reported for the virus (Jensen et al, 1991;Zhang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China

Wang

Huan

et al. 2013

J. Zhejiang Univ. Sci. B

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Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, purified MCMV particles were used as the immunogen to produce monoclonal antibodies (MAbs) and polyclonal antibodies (PAbs). Four murine MAbs (4B8, 8C11, 6F4, and 9G1) against MCMV were obtained through the hybridoma technology. The triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), dot-immunobinding assay (DIBA), and immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) using the MAb 4B8 were then developed for sensitive, specific, and rapid detection of MCMV in fields. MCMV could be detected in infected leaf crude extracts at dilutions of 1:327 680, 1:64 000, and 1:3 276 800 (w/v, g/ml) by TAS-ELISA, DIBA, and IC-RT-PCR, respectively. One hundred and sixty-one maize field samples showing virus-like symptoms and sixty-nine symptomless maize field samples from ten different provinces of China were collected and screened for the presence of MCMV using the established serological methods. A phylogenetic tree was constructed based on the full length CP genes and Chinese MCMV isolates formed one branch with Thailand isolates. The detection results demonstrated that MCMV is one of most prevalent viruses infecting maize in the Yunnan and Sichuan provinces of China.

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Biology and Control of Maize Chlorotic Mottle Virus

Cited by 59 publications

References 0 publications

Status of maize lethal necrosis in eastern Uganda

Status of maize lethal necrosis in eastern Uganda

Genetic analysis of tropical maize inbred lines for resistance to maize lethal necrosis disease

Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China

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