Biology, Genome Organization, and Evolution of Parvoviruses in Marine Shrimp

Dhar, Arun K.; Robles‐Sikisaka, Refugio; Saksmerprome, Vanvimon; Lakshman, Dilip K.

doi:10.1016/b978-0-12-800172-1.00003-3

Cited by 37 publications

(32 citation statements)

References 155 publications

(199 reference statements)

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“…Despite of the broad host range of densoviruses in insects, none so far have been reported in bees. The relation of densoviruses of the subfamily Densovirinae to their arthropod host ranges from mutualistic ( Xu et al, 2014 ) to severe pathology ( Dhar et al, 2014 ), which is especially problematic in large insect rearing facilities ( Szelei et al, 2011 ). In this respect, it is worth to investigate the biological relationship between the densoviruses that were discovered here and the commercial pollinator species B. terrestris .…”

Section: Resultsmentioning

confidence: 99%

Study of the Metatranscriptome of Eight Social and Solitary Wild Bee Species Reveals Novel Viruses and Bee Parasites

et al. 2018

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Bees are associated with a remarkable diversity of microorganisms, including unicellular parasites, bacteria, fungi, and viruses. The application of next-generation sequencing approaches enables the identification of this rich species composition as well as the discovery of previously unknown associations. Using high-throughput polyadenylated ribonucleic acid (RNA) sequencing, we investigated the metatranscriptome of eight wild bee species (Andrena cineraria, Andrena fulva, Andrena haemorrhoa, Bombus terrestris, Bombus cryptarum, Bombus pascuorum, Osmia bicornis, and Osmia cornuta) sampled from four different localities in Belgium. Across the RNA sequencing libraries, 88–99% of the taxonomically informative reads were of the host transcriptome. Four viruses with homology to insect pathogens were found including two RNA viruses (belonging to the families Iflaviridae and Tymoviridae that harbor already viruses of honey bees), a double stranded DNA virus (family Nudiviridae) and a single stranded DNA virus (family Parvoviridae). In addition, we found genomic sequences of 11 unclassified arthropod viruses (related to negeviruses, sobemoviruses, totiviruses, rhabdoviruses, and mononegaviruses), seven plant pathogenic viruses, and one fungal virus. Interestingly, nege-like viruses appear to be widespread, host-specific, and capable of attaining high copy numbers inside bees. Next to viruses, three novel parasite associations were discovered in wild bees, including Crithidia pragensis and a tubulinosematid and a neogregarine parasite. Yeasts of the genus Metschnikowia were identified in solitary bees. This study gives a glimpse of the microorganisms and viruses associated with social and solitary wild bees and demonstrates that their diversity exceeds by far the subset of species first discovered in honey bees.

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Section: Resultsmentioning

confidence: 99%

Study of the Metatranscriptome of Eight Social and Solitary Wild Bee Species Reveals Novel Viruses and Bee Parasites

et al. 2018

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“…We now need to investigate the role of PDK1 and ERK/ MAPK pathways during JcDV infection as their role in parvovirus infection has already been already shown (Riolobos et al, 2010;Chen et al, 2011;Bär et al, 2015). Interestingly, a previous study showed that p70S6K was cleaved by caspase-3 at an unconventional motif during DNA damageinduced apoptosis (DNA damage response; DDR; Dhar et al, 2014). It has been shown that DDR is activated during parvovirus replication and is required for infection (Adeyemi et al, 2010;Cotmore & Tattersall, 2013); p70S6K cleavage might be an outcome of JcDV-induced DDR activation.…”

Section: Discussionmentioning

confidence: 99%

Role of the phosphatidylinositol-3-kinase/Akt/target of rapamycin pathway during ambidensovirus infection of insect cells

Salasc

Mutuel

Debaisieux

et al. 2016

Journal of General Virology

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The phosphatidylinositol-3-kinase (PI3K)/Akt/target of rapamycin (TOR) signalling pathway controls cell growth and survival, and is targeted by a number of viruses at different phases of their infection cycle to control translation. Whether and how insect viruses interact with this pathway remain poorly addressed. Here, we investigated the role of PI3K/Akt/TOR signalling during lethal infection of insect cells with an insect parvovirus. Using Junonia coenia densovirus (JcDV; lepidopteran ambidensovirus 1) and susceptible insect cells as experimental models, we first described JcDV cytopathology, and showed that viral infection affects cell size, cell proliferation and survival. We deciphered the role of PI3K/Akt/TOR signalling in the course of infection and found that non-structural (NS) protein expression correlates with the inhibition of TOR and the shutdown of cellular synthesis, concomitant with the burst of viral protein expression. Together, these results suggest that NS proteins control the cellular translational machinery to favour the translation of viral mRNAs at the expense of cellular mRNAs. As a consequence of TOR inhibition, cell autophagy is activated. These results highlight new functions for NS proteins in the course of multiplication of an insect parvovirus.

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“…The subfamily of Densovirinae within the family Parvoviridae is a group of small (18–26 nm diameter), non-enveloped, icosahedral viruses containing a linear single-stranded DNA genome ranging between 4 and 6 kb with characteristic terminal hairpins [ 1 – 3 ]. Members of this subfamily typically produce “cellular dense nucleosis” pathogenesis in their hosts, hence, they are commonly termed densoviruses (DVs) [ 4 – 8 ].…”

Section: Introductionmentioning

confidence: 99%

Structure and transcription of the Helicoverpa armigera densovirus (HaDV2) genome and its expression strategy in LD652 cells

Graham

Wilson

et al. 2017

Virol J

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BackgroundDensoviruses (DVs) are highly pathogenic to their hosts. However, we previously reported a mutualistic DV (HaDV2). Very little was known about the characteristics of this virus, so herein we undertook a series of experiments to explore the molecular biology of HaDV2 further.ResultsPhylogenetic analysis showed that HaDV2 was similar to members of the genus Iteradensovirus. However, compared to current members of the genus Iteradensovirus, the sequence identity of HaDV2 is less than 44% at the nucleotide-level, and lower than 36, 28 and 19% at the amino-acid-level of VP, NS1 and NS2 proteins, respectively. Moreover, NS1 and NS2 proteins from HaDV2 were smaller than those from other iteradensoviruses due to their shorter N-terminal sequences. Two transcripts of about 2.2 kb coding for the NS proteins and the VP proteins were identified by Northern Blot and RACE analysis. Using specific anti-NS1 and anti-NS2 antibodies, Western Blot analysis revealed a 78 kDa and a 48 kDa protein, respectively. Finally, the localization of both NS1 and NS2 proteins within the cell nucleus was determined by using Green Fluorescent Protein (GFP) labelling.ConclusionThe genome organization, terminal hairpin structure, transcription and expression strategies as well as the mutualistic relationship with its host, suggested that HaDV2 was a novel member of the genus Iteradensovirus within the subfamily Densovirinae.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-017-0691-y) contains supplementary material, which is available to authorized users.

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Biology, Genome Organization, and Evolution of Parvoviruses in Marine Shrimp

Cited by 37 publications

References 155 publications

Study of the Metatranscriptome of Eight Social and Solitary Wild Bee Species Reveals Novel Viruses and Bee Parasites

Study of the Metatranscriptome of Eight Social and Solitary Wild Bee Species Reveals Novel Viruses and Bee Parasites

Role of the phosphatidylinositol-3-kinase/Akt/target of rapamycin pathway during ambidensovirus infection of insect cells

Structure and transcription of the Helicoverpa armigera densovirus (HaDV2) genome and its expression strategy in LD652 cells

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