Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells

Dégano, Irene R.; Vilalta, Marta; Bagó, Juli R.; Matthies, Annette M.; Hubbell, Jeffrey A.; Dimitriou, Helen; Bianco, Paolo; Rubio, Núria; Blanco, Jerónimo

doi:10.1016/j.biomaterials.2007.10.006

Cited by 81 publications

(68 citation statements)

References 25 publications

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“…Thus, if a faster regeneration of bone is needed, other means of osteoinduction should be employed. Cell seeding (Puelacher et al 1996;Vacanti et al 2001;Pioletti et al 2006;Degano et al 2008) and growth factor (Zisch et al 2003;Seliktar et al 2004) impregnation of scaffold enhance the rate of bone formation by a great extent, and are possible solutions to accelerate the bone formation inside the scaffold. However, as mentioned in the introduction, this comes with an increased complexity, which may hamper clinical translation of bone tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Translation of Biomechanical Concepts in Bone Tissue Engineering: From Animal Study to Revision Knee Arthroplasty

Ghias¹,

Terrier²,

Jolles-Haeberli³

et al. 2012

Journal of Biomechanics

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Bone defects in revision knee arthroplasty are often located in load-bearing regions. The goal of this study was to determine whether a physiologic load could be used as an in situ osteogenic signal to the scaffolds filling the bone defects. In order to answer this question, we proposed a novel translation procedure having four steps: (1) determining the mechanical stimulus using finite element method, (2) designing an animal study to measure bone formation spatially and temporally using micro-CT imaging in the scaffold subjected to the estimated mechanical stimulus, (3) identifying bone formation parameters for the loaded and non-loaded cases appearing in a recently developed mathematical model for bone formation in the scaffold and (4) estimating the stiffness and the bone formation in the bone-scaffold construct. With this procedure, we estimated that after 3 years mechanical stimulation increases the bone volume fraction and the stiffness of scaffold by 1.5-and 2.7-fold, respectively, compared to a non-loaded situation.

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Section: Discussionmentioning

confidence: 99%

Translation of Biomechanical Concepts in Bone Tissue Engineering: From Animal Study to Revision Knee Arthroplasty

Ghias¹,

Terrier²,

Jolles-Haeberli³

et al. 2012

Journal of Biomechanics

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“…As human bone marrow MSCs have the capacity to differentiate into osteoblasts, 51 this approach allows to investigate autologous bone repair and precise quantification of bone metastatic growth in vivo . 52,53 Although there are a number of molecular probes targeting bone formation, only one probe is available that targets osteoclast using a cathepsin K-activatable near-infrared fluorescence probe. In the presence of cathepsin K, side groups which are otherwise quenched are cleaved off from the probe and emit fluorescence.…”

Section: Time-lapsed Imaging Of Bone Remodeling Activitymentioning

confidence: 99%

Advances in multimodality molecular imaging of bone structure and function

2012

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The skeleton is important to the body as a source of minerals and blood cells and provides a structural framework for strength, mobility and the protection of organs. Bone diseases and disorders can have deteriorating effects on the skeleton, but the biological processes underlying anatomical changes in bone diseases occurring in vivo are not well understood, mostly due to the lack of appropriate analysis techniques. Therefore, there is ongoing research in the development of novel in vivo imaging techniques and molecular markers that might help to gain more knowledge of these pathological pathways in animal models and patients. This perspective provides an overview of the latest developments in molecular imaging applied to bone. It emphasizes that multimodality imaging, the combination of multiple imaging techniques encompassing different image modalities, enhances the interpretability of data, and is imperative for the understanding of the biological processes and the associated changes in bone structure and function relationships in vivo .

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“…Lentiviral production was performed as described previously [11,20]. The UCBMSCs were cotransduced (2 3 10 6 transducing units per milliliter, multiplicity of infection = 21, 48 hours) with the following lentiviral vectors: CMVp-RLuc-mRFP1, which contains a chimeric construct of the Renilla reniformis luciferase (RLuc) reporter gene and monomeric red fluorescent protein (mRFP1) in a PHR lentiviral vector under transcriptional control of the cytomegalovirus (CMV) promoter [21]; and CD31p-PLuc-eGFP, a fusion reporter vector composed of Photinus pyralis luciferase (PLuc) and enhanced green fluorescent protein (eGFP) coding regions under the transcriptional control of the 0.25-kb NorI/PstI fragment of the human CD31 promoter, which has higher transcriptional activity in endothelial cells than in monocytic cells [22].…”

Section: Genetic Modification Of Ucbmscsmentioning

confidence: 99%

Postinfarction Functional Recovery Driven by a Three-Dimensional Engineered Fibrin Patch Composed of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells

Roura

Soler‐Botija

Bagó

et al. 2015

Stem Cells Translational Medicine

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Considerable research has been dedicated to restoring myocardial cell slippage and limiting ventricular remodeling after myocardial infarction (MI). We examined the ability of a three-dimensional (3D) engineered fibrin patch filled with human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) to induce recovery of cardiac function after MI. The UCBMSCs were modified to coexpress luciferase and fluorescent protein reporters, mixed with fibrin, and applied as an adhesive, viable construct (fibrin-cell patch) over the infarcted myocardium in mice (MI-UCBMSC group). The patch adhered well to the heart. Noninvasive bioluminescence imaging demonstrated early proliferation and differentiation of UCBMSCs within the construct in the postinfarct mice in the MI-UCBMSC group. The implanted cells also participated in the formation of new, functional microvasculature that connected the fibrin-cell patch to both the subjacent myocardial tissue and the host circulatory system. As revealed by echocardiography, the left ventricular ejection fraction and fractional shortening at sacrifice were improved in MI-UCBMSC mice and were markedly reduced in mice treated with fibrin alone and untreated postinfarction controls. In conclusion, a 3D engineered fibrin patch composed of UCBMSCs attenuated infarct-derived cardiac dysfunction when transplanted locally over a myocardial wound. STEM CELLS TRANSLATIONAL MEDICINE 2015;4:956-966 SIGNIFICANCEIschemic heart failure (HF) is the end stage of many cardiovascular diseases, including myocardial infarction. The only definitive treatment for HF is cardiac transplant, which is hampered by limited number of heart donors and graft rejection. In recent times, cellular cardiomyoplasty has been expected to repair infarcted myocardium by implantation of different sources of stem or progenitor cells. However, low cell survival and myocardial implantation rates have motivated the emergence of novel approaches with the objective of generating graftable cell-based implants. Here, the potential of 3D engineered fibrin-umbilical cord blood-derived mesenchymal stem cells patches is shown to significantly recover lost general functions in post-infarcted mice.

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Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells

Cited by 81 publications

References 25 publications

Translation of Biomechanical Concepts in Bone Tissue Engineering: From Animal Study to Revision Knee Arthroplasty

Translation of Biomechanical Concepts in Bone Tissue Engineering: From Animal Study to Revision Knee Arthroplasty

Advances in multimodality molecular imaging of bone structure and function

Postinfarction Functional Recovery Driven by a Three-Dimensional Engineered Fibrin Patch Composed of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells

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