Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma

Bashmakova, Eugenia E.; Krasitskaya, Vasilisa V.; Zamay, Galina S.; Zamay, Tatiana N.; Frank, Ludmila A.

doi:10.1016/j.talanta.2019.03.030

Cited by 10 publications

(6 citation statements)

References 20 publications

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“…其次, 核酸适配体的生物相容性好、性质稳定, 对纳米材料的功能化能够降低纳米材料的毒性同时还能增强纳米材料的血液循环能力 [10] . 最重要的是, 核酸适配体的结合目标广, 可以用于多种目标物的测定, 包括金属离子 [11] 、小分子(如三磷酸腺苷(ATP) [12] )、生物大分子(如蛋白质 [13] 、核酸 [14] )、细胞 [15] 以及细菌 [16] , 极大地拓展了纳米荧光探针的检测范围. 因此, 基于核酸功能化的稀土基纳米材料构建的纳米荧光探针不仅稳定性和生物相容性好, 并且能够极大增强与靶标物的结合能力, 从而实现在复杂生物体系中对靶标物的检测.…”

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Bioassay Applications of Aptamer-Functionalized Rare Earth Nanomaterials

Jia¹,

Wang²,

Liang³

et al. 2020

Acta Chim. Sinica

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The levels of some biomolecules and ions in the body are usually related to the structural and functional changes of cells, tissues, organs, etc., which directly affect the prevention, diagnosis, and treatment of diseases. Therefore, in vivo bioassays of these substances are of great significance in medical and healthcare fields. The nano fluorescent probes consisted of rare earth nano materials have advantages of high sensitivity, simplicity, efficiency, and strong anti-interference ability, thus showing great potential in bioassays. The functionalization of aptamers on rare earth nanomaterials can further provide better specific recognition ability and biocompatibility for nano fluorescent probes, thereby enhancing their bioassays ability in complex samples. In this paper, the research progress of aptamer-functionalized rare earth nanomaterials as nano fluorescent probes in the field of bioassays is reviewed, and the main types, properties, detection mechanisms and detection substances are briefly introduced.

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Bioassay Applications of Aptamer-Functionalized Rare Earth Nanomaterials

Jia¹,

Wang²,

Liang³

et al. 2020

Acta Chim. Sinica

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“…Zamay et al used a targeted selection of DNA aptamers and applied two different aptamer clones for the isolation and detection of CTCs in peripheral blood samples from patients with different lung cancer types and benign lung tumors [ 130 ]. In addition, these aptamers were further utilized for a bioluminescent solid-phase sandwich-type microassay to detect lung tumor elements circulating in the blood [ 131 ]. Fang’s group introduced a cocktail of aptamer-modified magnetic nanoparticles (apt-MNPs) to capture heterogeneous CTC populations [ 132 ].…”

Section: Development Of Cancer-specific Aptamers For Diagnosis and Treatmentmentioning

confidence: 99%

The Potential of Aptamer-Mediated Liquid Biopsy for Early Detection of Cancer

Roy¹,

Pascher

Juratli

et al. 2021

IJMS

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The early detection of cancer favors a greater chance of curative treatment and long-term survival. Exciting new technologies have been developed that can help to catch the disease early. Liquid biopsy is a promising non-invasive tool to detect cancer, even at an early stage, as well as to continuously monitor disease progression and treatment efficacy. Various methods have been implemented to isolate and purify bio-analytes in liquid biopsy specimens. Aptamers are short oligonucleotides consisting of either DNA or RNA that are capable of binding to target molecules with high specificity. Due to their unique properties, they are considered promising recognition ligands for the early detection of cancer by liquid biopsy. A variety of circulating targets have been isolated with high affinity and specificity by facile modification and affinity regulation of the aptamers. In this review, we discuss recent progress in aptamer-mediated liquid biopsy for cancer detection, its associated challenges, and its future potential for clinical applications.

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“…Obelin chemically conjugated with specific DNA aptamers was applied as a label in sandwich-type solid-phase analysis of lung tumor elements in clinical plasma. The developed assay demonstrated good sensitivity of 91.5% and specificity of 75% ( p < 0.001) [ 78 ]. Analogous conjugate with (2′-F-Py) modified RNA aptamer to the multiple sclerosis (MS) related autoantibodies was used for detection of autoantibodies to myelin basic protein in sera samples from MS-diagnosed patients (91) and non-MS donors (81).…”

Section: Analytical Application Of Ca 2+ -Regulmentioning

confidence: 99%

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Krasitskaya

Bashmakova

Frank

2020

IJMS

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: The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.

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Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma

Cited by 10 publications

References 20 publications

Bioassay Applications of Aptamer-Functionalized Rare Earth Nanomaterials

Bioassay Applications of Aptamer-Functionalized Rare Earth Nanomaterials

The Potential of Aptamer-Mediated Liquid Biopsy for Early Detection of Cancer

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

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